Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1

Yuki Enoki, Hiroshi Watanabe, Riho Arake, Ryusei Sugimoto, Tadashi Imafuku, Yuna Tominaga, Yu Ishima, Shunsuke Kotani, Makoto Nakajima, Motoko Tanaka, Kazutaka Matsushita, Masafumi Fukagawa, Masaki Otagiri, Toru Maruyama

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Skeletal muscle atrophy, referred to as sarcopenia, is often observed in chronic kidney disease (CKD) patients, especially in patients who are undergoing hemodialysis. The purpose of this study was to determine whether uremic toxins are involved in CKD-related skeletal muscle atrophy. Among six protein-bound uremic toxins, indole containing compounds, indoxyl sulfate (IS) significantly inhibited proliferation and myotube formation in C2C12 myoblast cells. IS increased the factors related to skeletal muscle breakdown, such as reactive oxygen species (ROS) and inflammatory cytokines (TNF-α, IL-6 and TGF-β1) in C2C12 cells. IS also enhanced the production of muscle atrophy-related genes, myostatin and atrogin-1. These effects induced by IS were suppressed in the presence of an antioxidant or inhibitors of the organic anion transporter and aryl hydrocarbon receptor. The administered IS was distributed to skeletal muscle and induced superoxide production in half-nephrectomized (1/2 Nx) mice. The chronic administration of IS significantly reduced the body weights accompanied by skeletal muscle weight loss. Similar to the in vitro data, IS induced the expression of myostatin and atrogin-1 in addition to increasing the production of inflammatory cytokines by enhancing oxidative stress in skeletal muscle. These data suggest that IS has the potential to accelerate skeletal muscle atrophy by inducing oxidative stress-mediated myostatin and atrogin-1 expression.

Original languageEnglish
Article number32084
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016 Aug 23
Externally publishedYes

Fingerprint

Indican
Myostatin
Muscular Atrophy
Skeletal Muscle
Oxidative Stress
Chronic Renal Insufficiency
Organic Anion Transporters
Cytokines
Sarcopenia
Aryl Hydrocarbon Receptors
Myoblasts
Skeletal Muscle Fibers
Superoxides
Renal Dialysis
Weight Loss
Interleukin-6
Reactive Oxygen Species
Antioxidants
Body Weight

ASJC Scopus subject areas

  • General

Cite this

Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1. / Enoki, Yuki; Watanabe, Hiroshi; Arake, Riho; Sugimoto, Ryusei; Imafuku, Tadashi; Tominaga, Yuna; Ishima, Yu; Kotani, Shunsuke; Nakajima, Makoto; Tanaka, Motoko; Matsushita, Kazutaka; Fukagawa, Masafumi; Otagiri, Masaki; Maruyama, Toru.

In: Scientific Reports, Vol. 6, 32084, 23.08.2016.

Research output: Contribution to journalArticle

Enoki, Y, Watanabe, H, Arake, R, Sugimoto, R, Imafuku, T, Tominaga, Y, Ishima, Y, Kotani, S, Nakajima, M, Tanaka, M, Matsushita, K, Fukagawa, M, Otagiri, M & Maruyama, T 2016, 'Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1', Scientific Reports, vol. 6, 32084. https://doi.org/10.1038/srep32084
Enoki, Yuki ; Watanabe, Hiroshi ; Arake, Riho ; Sugimoto, Ryusei ; Imafuku, Tadashi ; Tominaga, Yuna ; Ishima, Yu ; Kotani, Shunsuke ; Nakajima, Makoto ; Tanaka, Motoko ; Matsushita, Kazutaka ; Fukagawa, Masafumi ; Otagiri, Masaki ; Maruyama, Toru. / Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1. In: Scientific Reports. 2016 ; Vol. 6.
@article{d620f469244445729717d983b868f9d7,
title = "Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1",
abstract = "Skeletal muscle atrophy, referred to as sarcopenia, is often observed in chronic kidney disease (CKD) patients, especially in patients who are undergoing hemodialysis. The purpose of this study was to determine whether uremic toxins are involved in CKD-related skeletal muscle atrophy. Among six protein-bound uremic toxins, indole containing compounds, indoxyl sulfate (IS) significantly inhibited proliferation and myotube formation in C2C12 myoblast cells. IS increased the factors related to skeletal muscle breakdown, such as reactive oxygen species (ROS) and inflammatory cytokines (TNF-α, IL-6 and TGF-β1) in C2C12 cells. IS also enhanced the production of muscle atrophy-related genes, myostatin and atrogin-1. These effects induced by IS were suppressed in the presence of an antioxidant or inhibitors of the organic anion transporter and aryl hydrocarbon receptor. The administered IS was distributed to skeletal muscle and induced superoxide production in half-nephrectomized (1/2 Nx) mice. The chronic administration of IS significantly reduced the body weights accompanied by skeletal muscle weight loss. Similar to the in vitro data, IS induced the expression of myostatin and atrogin-1 in addition to increasing the production of inflammatory cytokines by enhancing oxidative stress in skeletal muscle. These data suggest that IS has the potential to accelerate skeletal muscle atrophy by inducing oxidative stress-mediated myostatin and atrogin-1 expression.",
author = "Yuki Enoki and Hiroshi Watanabe and Riho Arake and Ryusei Sugimoto and Tadashi Imafuku and Yuna Tominaga and Yu Ishima and Shunsuke Kotani and Makoto Nakajima and Motoko Tanaka and Kazutaka Matsushita and Masafumi Fukagawa and Masaki Otagiri and Toru Maruyama",
year = "2016",
month = "8",
day = "23",
doi = "10.1038/srep32084",
language = "English",
volume = "6",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Indoxyl sulfate potentiates skeletal muscle atrophy by inducing the oxidative stress-mediated expression of myostatin and atrogin-1

AU - Enoki, Yuki

AU - Watanabe, Hiroshi

AU - Arake, Riho

AU - Sugimoto, Ryusei

AU - Imafuku, Tadashi

AU - Tominaga, Yuna

AU - Ishima, Yu

AU - Kotani, Shunsuke

AU - Nakajima, Makoto

AU - Tanaka, Motoko

AU - Matsushita, Kazutaka

AU - Fukagawa, Masafumi

AU - Otagiri, Masaki

AU - Maruyama, Toru

PY - 2016/8/23

Y1 - 2016/8/23

N2 - Skeletal muscle atrophy, referred to as sarcopenia, is often observed in chronic kidney disease (CKD) patients, especially in patients who are undergoing hemodialysis. The purpose of this study was to determine whether uremic toxins are involved in CKD-related skeletal muscle atrophy. Among six protein-bound uremic toxins, indole containing compounds, indoxyl sulfate (IS) significantly inhibited proliferation and myotube formation in C2C12 myoblast cells. IS increased the factors related to skeletal muscle breakdown, such as reactive oxygen species (ROS) and inflammatory cytokines (TNF-α, IL-6 and TGF-β1) in C2C12 cells. IS also enhanced the production of muscle atrophy-related genes, myostatin and atrogin-1. These effects induced by IS were suppressed in the presence of an antioxidant or inhibitors of the organic anion transporter and aryl hydrocarbon receptor. The administered IS was distributed to skeletal muscle and induced superoxide production in half-nephrectomized (1/2 Nx) mice. The chronic administration of IS significantly reduced the body weights accompanied by skeletal muscle weight loss. Similar to the in vitro data, IS induced the expression of myostatin and atrogin-1 in addition to increasing the production of inflammatory cytokines by enhancing oxidative stress in skeletal muscle. These data suggest that IS has the potential to accelerate skeletal muscle atrophy by inducing oxidative stress-mediated myostatin and atrogin-1 expression.

AB - Skeletal muscle atrophy, referred to as sarcopenia, is often observed in chronic kidney disease (CKD) patients, especially in patients who are undergoing hemodialysis. The purpose of this study was to determine whether uremic toxins are involved in CKD-related skeletal muscle atrophy. Among six protein-bound uremic toxins, indole containing compounds, indoxyl sulfate (IS) significantly inhibited proliferation and myotube formation in C2C12 myoblast cells. IS increased the factors related to skeletal muscle breakdown, such as reactive oxygen species (ROS) and inflammatory cytokines (TNF-α, IL-6 and TGF-β1) in C2C12 cells. IS also enhanced the production of muscle atrophy-related genes, myostatin and atrogin-1. These effects induced by IS were suppressed in the presence of an antioxidant or inhibitors of the organic anion transporter and aryl hydrocarbon receptor. The administered IS was distributed to skeletal muscle and induced superoxide production in half-nephrectomized (1/2 Nx) mice. The chronic administration of IS significantly reduced the body weights accompanied by skeletal muscle weight loss. Similar to the in vitro data, IS induced the expression of myostatin and atrogin-1 in addition to increasing the production of inflammatory cytokines by enhancing oxidative stress in skeletal muscle. These data suggest that IS has the potential to accelerate skeletal muscle atrophy by inducing oxidative stress-mediated myostatin and atrogin-1 expression.

UR - http://www.scopus.com/inward/record.url?scp=84984682149&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84984682149&partnerID=8YFLogxK

U2 - 10.1038/srep32084

DO - 10.1038/srep32084

M3 - Article

VL - 6

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 32084

ER -