Induction and stimulation of 92-kDa gelatinase / type IV collagenase production in osteosarcoma and fibrosarcoma cell lines by tumor necrosis factor α

Yasunori Okada, Hiroyuki Tsuchiya, Hirokazu Shimizu, Katsuro Tomita, Isao Nakanishi, Hiroshi Sato, Motoharu Seiki, Kyoko Yamashita, Taro Hayakawa

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106 Citations (Scopus)


Production of a 92-kDa gelatinase / type IV collagenase and tissue inhibitor of metalloproteinases (TIMP) was investigated with human sarcoma cell lines. Among the cytokines and growth factors examined, only human recombinant tumor necrosis factor α (TNFα) induced and stimulated the proteinase with concomitant increase in TIMP expression, but matrix metalloproteinase 2 (72-kDa gelatinase / type IV collagenase) expression was unchanged. These data suggest that gene expression of the two metalloproteinases is regulated in a different fashion and TNFα may be important to allow cancer cells to be more invasive and metastatic.

Original languageEnglish
Pages (from-to)610-617
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 1990 Sep 14


ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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