Induction of adipogenic differentiation in three-dimensional culture model on a novel microfabricated scaffold

Yoshitaka Miyagawa, Hajime Okita, Nobutaka Kiyokawa

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

The development of a three-dimensional (3D) culture system is very important for regenerative medicine and drug discovery applications of stem cell technology because the 3D culture condition could mimic the stem cell environment in vivo and support accurate differentiation. This chapter describes a novel 3D culture technique for efficient induction of adipogenic differentiation. This 3D culture system provides an easy way to allow cells to form a 3D spheroid structure without any matrix derived from animal and chemical substances. We firstly describe the details of the 3D culture technique using human mesenchymal stem/progenitor cells (MPCs) and its optimization. Then we elaborate on the protocol of efficient induction for adipogenic differentiation and adipocyte-specific gene expression, including peroxisome proliferator-activated receptor-γ, with our culture system in human MPCs.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages275-286
Number of pages12
Volume952
DOIs
Publication statusPublished - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume952
ISSN (Print)10643745

Fingerprint

Mesenchymal Stromal Cells
Culture Techniques
Stem Cells
Peroxisome Proliferator-Activated Receptors
Regenerative Medicine
Drug Discovery
Adipocytes
Technology
Gene Expression

Keywords

  • Adipogenesis
  • Human mesenchymal stem/progenitor cells
  • Microfabricated scaffold
  • Peroxisome proliferator-activated receptor-γ
  • Three-dimensional culture

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Miyagawa, Y., Okita, H., & Kiyokawa, N. (2013). Induction of adipogenic differentiation in three-dimensional culture model on a novel microfabricated scaffold. In Methods in Molecular Biology (Vol. 952, pp. 275-286). (Methods in Molecular Biology; Vol. 952). https://doi.org/10.1007/978-1-62703-155-4_20

Induction of adipogenic differentiation in three-dimensional culture model on a novel microfabricated scaffold. / Miyagawa, Yoshitaka; Okita, Hajime; Kiyokawa, Nobutaka.

Methods in Molecular Biology. Vol. 952 2013. p. 275-286 (Methods in Molecular Biology; Vol. 952).

Research output: Chapter in Book/Report/Conference proceedingChapter

Miyagawa, Y, Okita, H & Kiyokawa, N 2013, Induction of adipogenic differentiation in three-dimensional culture model on a novel microfabricated scaffold. in Methods in Molecular Biology. vol. 952, Methods in Molecular Biology, vol. 952, pp. 275-286. https://doi.org/10.1007/978-1-62703-155-4_20
Miyagawa Y, Okita H, Kiyokawa N. Induction of adipogenic differentiation in three-dimensional culture model on a novel microfabricated scaffold. In Methods in Molecular Biology. Vol. 952. 2013. p. 275-286. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-155-4_20
Miyagawa, Yoshitaka ; Okita, Hajime ; Kiyokawa, Nobutaka. / Induction of adipogenic differentiation in three-dimensional culture model on a novel microfabricated scaffold. Methods in Molecular Biology. Vol. 952 2013. pp. 275-286 (Methods in Molecular Biology).
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