Induction of CD8+ T cell-mediated protective immunity against Trypanosoma cruzi

Yasushi Miyahira, Seiki Kobayashi, Tsutomu Takeuchi, Tsuneo Kamiyama, Takeshi Nara, Junko Nakajima-Shimada, Takashi Aoki

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Trypanosoma cruzi was transformed with the Plasmodium yoelii gene encoding the circumsporozoite (CS) protein, which contains the well-characterized CD8+ T cell epitope, SYVPSAEQI. In vivo and in vitro assays indicated that cells infected with the transformed T. cruzi could process and present this malaria parasite-derived class I MHC-restricted epitope. Immunization of mice with recombinant influenza and vaccinia viruses expressing the SYVPSAEQI epitope induced a large number of specific CD8+ T cells that strongly suppressed parasitemia and conferred complete protection against the acute T. cruzi lethal infection. CD8+ T cells mediated this immunity as indicated by the unrelenting parasitemia and high mortality observed in immunized mice treated with anti-CD8 antibody. This study demonstrated, for the first time, that vaccination of mice with vectors designed to induce CD8+ T cells is effective against T. cruzi infection.

Original languageEnglish
Pages (from-to)133-141
Number of pages9
JournalInternational immunology
Volume11
Issue number2
DOIs
Publication statusPublished - 1999 Feb 17

Keywords

  • CD8 T cells
  • Chagas' disease
  • Protective immunity
  • Recombinant influenza virus
  • Recombinant vaccinia virus
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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  • Cite this

    Miyahira, Y., Kobayashi, S., Takeuchi, T., Kamiyama, T., Nara, T., Nakajima-Shimada, J., & Aoki, T. (1999). Induction of CD8+ T cell-mediated protective immunity against Trypanosoma cruzi. International immunology, 11(2), 133-141. https://doi.org/10.1093/intimm/11.2.133