TY - JOUR
T1 - Induction of CD8+ T cell-mediated protective immunity against Trypanosoma cruzi
AU - Miyahira, Yasushi
AU - Kobayashi, Seiki
AU - Takeuchi, Tsutomu
AU - Kamiyama, Tsuneo
AU - Nara, Takeshi
AU - Nakajima-Shimada, Junko
AU - Aoki, Takashi
PY - 1999
Y1 - 1999
N2 - Trypanosoma cruzi was transformed with the Plasmodium yoelii gene encoding the circumsporozoite (CS) protein, which contains the well-characterized CD8+ T cell epitope, SYVPSAEQI. In vivo and in vitro assays indicated that cells infected with the transformed T. cruzi could process and present this malaria parasite-derived class I MHC-restricted epitope. Immunization of mice with recombinant influenza and vaccinia viruses expressing the SYVPSAEQI epitope induced a large number of specific CD8+ T cells that strongly suppressed parasitemia and conferred complete protection against the acute T. cruzi lethal infection. CD8+ T cells mediated this immunity as indicated by the unrelenting parasitemia and high mortality observed in immunized mice treated with anti-CD8 antibody. This study demonstrated, for the first time, that vaccination of mice with vectors designed to induce CD8+ T cells is effective against T. cruzi infection.
AB - Trypanosoma cruzi was transformed with the Plasmodium yoelii gene encoding the circumsporozoite (CS) protein, which contains the well-characterized CD8+ T cell epitope, SYVPSAEQI. In vivo and in vitro assays indicated that cells infected with the transformed T. cruzi could process and present this malaria parasite-derived class I MHC-restricted epitope. Immunization of mice with recombinant influenza and vaccinia viruses expressing the SYVPSAEQI epitope induced a large number of specific CD8+ T cells that strongly suppressed parasitemia and conferred complete protection against the acute T. cruzi lethal infection. CD8+ T cells mediated this immunity as indicated by the unrelenting parasitemia and high mortality observed in immunized mice treated with anti-CD8 antibody. This study demonstrated, for the first time, that vaccination of mice with vectors designed to induce CD8+ T cells is effective against T. cruzi infection.
KW - CD8 T cells
KW - Chagas' disease
KW - Protective immunity
KW - Recombinant influenza virus
KW - Recombinant vaccinia virus
KW - Trypanosoma cruzi
UR - http://www.scopus.com/inward/record.url?scp=0033009955&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033009955&partnerID=8YFLogxK
U2 - 10.1093/intimm/11.2.133
DO - 10.1093/intimm/11.2.133
M3 - Article
C2 - 10069411
AN - SCOPUS:0033009955
VL - 11
SP - 133
EP - 141
JO - International Immunology
JF - International Immunology
SN - 0953-8178
IS - 2
ER -
