Induction of choline acetyltransferase mRNA in human mononuclear leukocytes stimulated by phytohemagglutinin, a T-cell activator

Takeshi Fujii, Shin Yamada, Yoshihiro Watanabe, Hidemi Misawa, Sonoko Tajima, Kazuko Fujimoto, Tadashi Kasahara, Koichiro Kawashima

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

The induction of mRNA for choline acetyltransferase (CHAT), which catalyzes acetylcholine (ACh) synthesis was investigated in human mononuclear leukocytes (MNL) stimulated by phytohemagglutinin (PHA), a T-cell activator, using the reverse transcription-polymerase chain reaction. Stimulation of MNL by PHA induced the expression of ChAT mRNA, and potentiated ACh synthesis. ChAT mRNA induction required more time than the induction of interleukin-2 mRNA. Expression of the gene encoding the vesicular ACh transporter, which mediates ACh transport in cholinergic neurons, was not observed in PHA- stimulated MNL, suggesting that the mechanisms controlling ACh release from T-lymphocytes differ from those in cholinergic neurons. These findings demonstrate that activation of T-lymphocytes up-regulates ACh synthesis in the blood, and suggest that ACh plays an important role as a neuroimmunomodulator besides its role as a neurotransmitter.

Original languageEnglish
Pages (from-to)101-107
Number of pages7
JournalJournal of Neuroimmunology
Volume82
Issue number1
DOIs
Publication statusPublished - 1998 Feb 1

Keywords

  • Acetylcholine
  • Choline acetyltransferase
  • Interleukin-2
  • Mononuclear leukocytes
  • Radioimmunoassay
  • T-lymphocyte

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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