Abstract
The induction of mRNA for choline acetyltransferase (CHAT), which catalyzes acetylcholine (ACh) synthesis was investigated in human mononuclear leukocytes (MNL) stimulated by phytohemagglutinin (PHA), a T-cell activator, using the reverse transcription-polymerase chain reaction. Stimulation of MNL by PHA induced the expression of ChAT mRNA, and potentiated ACh synthesis. ChAT mRNA induction required more time than the induction of interleukin-2 mRNA. Expression of the gene encoding the vesicular ACh transporter, which mediates ACh transport in cholinergic neurons, was not observed in PHA- stimulated MNL, suggesting that the mechanisms controlling ACh release from T-lymphocytes differ from those in cholinergic neurons. These findings demonstrate that activation of T-lymphocytes up-regulates ACh synthesis in the blood, and suggest that ACh plays an important role as a neuroimmunomodulator besides its role as a neurotransmitter.
| Original language | English |
|---|---|
| Pages (from-to) | 101-107 |
| Number of pages | 7 |
| Journal | Journal of Neuroimmunology |
| Volume | 82 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1998 Feb |
Fingerprint
Keywords
- Acetylcholine
- Choline acetyltransferase
- Interleukin-2
- Mononuclear leukocytes
- Radioimmunoassay
- T-lymphocyte
ASJC Scopus subject areas
- Immunology
- Clinical Neurology
- Immunology and Allergy
- Neurology
Cite this
Induction of choline acetyltransferase mRNA in human mononuclear leukocytes stimulated by phytohemagglutinin, a T-cell activator. / Fujii, Takeshi; Yamada, Shin; Watanabe, Yoshihiro; Misawa, Hidemi; Tajima, Sonoko; Fujimoto, Kazuko; Kasahara, Tadashi; Kawashima, Koichiro.
In: Journal of Neuroimmunology, Vol. 82, No. 1, 02.1998, p. 101-107.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of choline acetyltransferase mRNA in human mononuclear leukocytes stimulated by phytohemagglutinin, a T-cell activator
AU - Fujii, Takeshi
AU - Yamada, Shin
AU - Watanabe, Yoshihiro
AU - Misawa, Hidemi
AU - Tajima, Sonoko
AU - Fujimoto, Kazuko
AU - Kasahara, Tadashi
AU - Kawashima, Koichiro
PY - 1998/2
Y1 - 1998/2
N2 - The induction of mRNA for choline acetyltransferase (CHAT), which catalyzes acetylcholine (ACh) synthesis was investigated in human mononuclear leukocytes (MNL) stimulated by phytohemagglutinin (PHA), a T-cell activator, using the reverse transcription-polymerase chain reaction. Stimulation of MNL by PHA induced the expression of ChAT mRNA, and potentiated ACh synthesis. ChAT mRNA induction required more time than the induction of interleukin-2 mRNA. Expression of the gene encoding the vesicular ACh transporter, which mediates ACh transport in cholinergic neurons, was not observed in PHA- stimulated MNL, suggesting that the mechanisms controlling ACh release from T-lymphocytes differ from those in cholinergic neurons. These findings demonstrate that activation of T-lymphocytes up-regulates ACh synthesis in the blood, and suggest that ACh plays an important role as a neuroimmunomodulator besides its role as a neurotransmitter.
AB - The induction of mRNA for choline acetyltransferase (CHAT), which catalyzes acetylcholine (ACh) synthesis was investigated in human mononuclear leukocytes (MNL) stimulated by phytohemagglutinin (PHA), a T-cell activator, using the reverse transcription-polymerase chain reaction. Stimulation of MNL by PHA induced the expression of ChAT mRNA, and potentiated ACh synthesis. ChAT mRNA induction required more time than the induction of interleukin-2 mRNA. Expression of the gene encoding the vesicular ACh transporter, which mediates ACh transport in cholinergic neurons, was not observed in PHA- stimulated MNL, suggesting that the mechanisms controlling ACh release from T-lymphocytes differ from those in cholinergic neurons. These findings demonstrate that activation of T-lymphocytes up-regulates ACh synthesis in the blood, and suggest that ACh plays an important role as a neuroimmunomodulator besides its role as a neurotransmitter.
KW - Acetylcholine
KW - Choline acetyltransferase
KW - Interleukin-2
KW - Mononuclear leukocytes
KW - Radioimmunoassay
KW - T-lymphocyte
UR - http://www.scopus.com/inward/record.url?scp=0032006012&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032006012&partnerID=8YFLogxK
U2 - 10.1016/S0165-5728(97)00195-1
DO - 10.1016/S0165-5728(97)00195-1
M3 - Article
VL - 82
SP - 101
EP - 107
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - 1
ER -