TY - JOUR
T1 - Induction of choline acetyltransferase mRNA in human mononuclear leukocytes stimulated by phytohemagglutinin, a T-cell activator
AU - Fujii, Takeshi
AU - Yamada, Shin
AU - Watanabe, Yoshihiro
AU - Misawa, Hidemi
AU - Tajima, Sonoko
AU - Fujimoto, Kazuko
AU - Kasahara, Tadashi
AU - Kawashima, Koichiro
N1 - Funding Information:
This research was partly supported by a Grant-in-aid (No. 08772104) for Scientific Research from the Ministry of Education, Science and Culture, Japan.
PY - 1998/2
Y1 - 1998/2
N2 - The induction of mRNA for choline acetyltransferase (CHAT), which catalyzes acetylcholine (ACh) synthesis was investigated in human mononuclear leukocytes (MNL) stimulated by phytohemagglutinin (PHA), a T-cell activator, using the reverse transcription-polymerase chain reaction. Stimulation of MNL by PHA induced the expression of ChAT mRNA, and potentiated ACh synthesis. ChAT mRNA induction required more time than the induction of interleukin-2 mRNA. Expression of the gene encoding the vesicular ACh transporter, which mediates ACh transport in cholinergic neurons, was not observed in PHA- stimulated MNL, suggesting that the mechanisms controlling ACh release from T-lymphocytes differ from those in cholinergic neurons. These findings demonstrate that activation of T-lymphocytes up-regulates ACh synthesis in the blood, and suggest that ACh plays an important role as a neuroimmunomodulator besides its role as a neurotransmitter.
AB - The induction of mRNA for choline acetyltransferase (CHAT), which catalyzes acetylcholine (ACh) synthesis was investigated in human mononuclear leukocytes (MNL) stimulated by phytohemagglutinin (PHA), a T-cell activator, using the reverse transcription-polymerase chain reaction. Stimulation of MNL by PHA induced the expression of ChAT mRNA, and potentiated ACh synthesis. ChAT mRNA induction required more time than the induction of interleukin-2 mRNA. Expression of the gene encoding the vesicular ACh transporter, which mediates ACh transport in cholinergic neurons, was not observed in PHA- stimulated MNL, suggesting that the mechanisms controlling ACh release from T-lymphocytes differ from those in cholinergic neurons. These findings demonstrate that activation of T-lymphocytes up-regulates ACh synthesis in the blood, and suggest that ACh plays an important role as a neuroimmunomodulator besides its role as a neurotransmitter.
KW - Acetylcholine
KW - Choline acetyltransferase
KW - Interleukin-2
KW - Mononuclear leukocytes
KW - Radioimmunoassay
KW - T-lymphocyte
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U2 - 10.1016/S0165-5728(97)00195-1
DO - 10.1016/S0165-5728(97)00195-1
M3 - Article
C2 - 9526852
AN - SCOPUS:0032006012
VL - 82
SP - 101
EP - 107
JO - Advances in Neuroimmunology
JF - Advances in Neuroimmunology
SN - 0165-5728
IS - 1
ER -