Induction of choline acetyltransferase mRNA in human mononuclear leukocytes stimulated by phytohemagglutinin, a T-cell activator

Takeshi Fujii, Shin Yamada, Yoshihiro Watanabe, Hidemi Misawa, Sonoko Tajima, Kazuko Fujimoto, Tadashi Kasahara, Koichiro Kawashima

Research output: Contribution to journalArticlepeer-review

81 Citations (Scopus)

Abstract

The induction of mRNA for choline acetyltransferase (CHAT), which catalyzes acetylcholine (ACh) synthesis was investigated in human mononuclear leukocytes (MNL) stimulated by phytohemagglutinin (PHA), a T-cell activator, using the reverse transcription-polymerase chain reaction. Stimulation of MNL by PHA induced the expression of ChAT mRNA, and potentiated ACh synthesis. ChAT mRNA induction required more time than the induction of interleukin-2 mRNA. Expression of the gene encoding the vesicular ACh transporter, which mediates ACh transport in cholinergic neurons, was not observed in PHA- stimulated MNL, suggesting that the mechanisms controlling ACh release from T-lymphocytes differ from those in cholinergic neurons. These findings demonstrate that activation of T-lymphocytes up-regulates ACh synthesis in the blood, and suggest that ACh plays an important role as a neuroimmunomodulator besides its role as a neurotransmitter.

Original languageEnglish
Pages (from-to)101-107
Number of pages7
JournalJournal of Neuroimmunology
Volume82
Issue number1
DOIs
Publication statusPublished - 1998 Feb

Keywords

  • Acetylcholine
  • Choline acetyltransferase
  • Interleukin-2
  • Mononuclear leukocytes
  • Radioimmunoassay
  • T-lymphocyte

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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