Induction of different reactive oxygen species in the skin during various laser therapies and their inhibition by fullerene

Takahiro Fujimoto, Shinobu Ito, Masayuki Ito, Hideko Kanazawa, Shigeru Yamaguchi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background and Objectives The production of reactive oxygen species (ROS) is one of the mechanisms of laser irradiation in the skin, and there are beneficial and detrimental aspects to this reaction. Detrimental side effects after laser treatments, such as redness and pigmentation, can be reduced by using anti-oxidants. Materials and Methods Electron spin resonance (ESR) analysis using a free radical trapping agent revealed that different free radicals, including hydroxyl (·OH) and superoxide anion (O 2 -) radicals, were generated in the skin of hairless mice by irradiation with intense pulsed light (IPL), plasma, and radio frequency lasers. Results Generation of O 2 - and •OH radicals was significantly inhibited in a dose-dependent fashion by fullerene and fullerene did not have any pro-oxidant effects as no radical adduct signal was detected. Although ROS can increase expression of COX-2 mRNA, an inflammatory marker, laser-induced COX-2 expression was significantly suppressed by the antioxidant activity of fullerene. In addition, imaging analysis of human skin has shown that erythema-associated redness caused by laser-induced inflammation is inhibited by fullerene gel. Conclusion These data suggest that laser-induced inflammation is suppressed by the ROS-scavenging activity of fullerene and that application of fullerene is effective against oxidative skin damage caused by laser irradiation. Thus, fullerene has potential as an after-care therapy following laser irradiation of the skin.

Original languageEnglish
Pages (from-to)685-694
Number of pages10
JournalLasers in Surgery and Medicine
Volume44
Issue number8
DOIs
Publication statusPublished - 2012 Oct

Fingerprint

Fullerenes
Laser Therapy
Reactive Oxygen Species
Lasers
Skin
Free Radicals
Inflammation
Hairless Mouse
Electron Spin Resonance Spectroscopy
Pigmentation
Erythema
Radio
Oxidants
Superoxides
Hydroxyl Radical
Antioxidants
Gels
Light
Messenger RNA

Keywords

  • ascorbyl radical
  • fullerene
  • hydroxyl radical
  • laser irradiation
  • superoxide

ASJC Scopus subject areas

  • Surgery
  • Dermatology

Cite this

Induction of different reactive oxygen species in the skin during various laser therapies and their inhibition by fullerene. / Fujimoto, Takahiro; Ito, Shinobu; Ito, Masayuki; Kanazawa, Hideko; Yamaguchi, Shigeru.

In: Lasers in Surgery and Medicine, Vol. 44, No. 8, 10.2012, p. 685-694.

Research output: Contribution to journalArticle

Fujimoto, Takahiro ; Ito, Shinobu ; Ito, Masayuki ; Kanazawa, Hideko ; Yamaguchi, Shigeru. / Induction of different reactive oxygen species in the skin during various laser therapies and their inhibition by fullerene. In: Lasers in Surgery and Medicine. 2012 ; Vol. 44, No. 8. pp. 685-694.
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