Induction of DNA methylation by artificial piRNA production in male germ cells

Daisuke Itou, Yusuke Shiromoto, Yukiho Shin-ya, Chika Ishii, Toru Nishimura, Narumi Ogonuki, Atsuo Ogura, Hidetoshi Hasuwa, Yoshitaka Fujihara, Satomi Kuramochi-Miyagawa, Toru Nakano

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Global DNA demethylation and subsequent de novo DNA methylation take place in mammalian male embryonic germ cells [1-3]. P-elementinduced wimpy testis (PIWI)-interacting RNAs (piRNAs), which are germline-specific small RNAs, have been postulated to be critically important for de novo DNA methylation of retrotransposon genes, and many proteins, including PIWI family proteins, play pivotal roles in this process [4-6]. In the embryonic mouse testis, two mouse PIWI proteins, mouse PIWI-like (MILI) and mouse PIWI2 (MIWI2), are involved in the biogenesis of piRNAs through the so-called ping-pong amplification cycle [7-10], and long single-stranded RNAs transcribed from the gene regions of piRNA clusters have been proposed to be the initial material [11-16]. However, it remains unclear whether transcription from the piRNA clusters is required for the biogenesis of piRNAs. To answer this question, we developed a novel artificial piRNA production system by simple expression of sense and antisense EGFP mRNAs in embryonic male germ cells in the piRNA biogenesis phase. EGFP expression was silenced by piRNA-dependent DNA methylation, indicating that concomitant expression of sense and antisense RNA transcripts is necessary and sufficient for piRNA production and subsequent piRNA-dependent gene silencing. In addition, we demonstrated that this artificial piRNA induction paradigm could be applied to an endogenous gene essential for spermatogenesis, DNM T3L [3, 17, 18]. This study not only provides novel insights into the molecular mechanisms of piRNA production, but also presents an innovative strategy for inducing epigenetic modification in germ cells.

Original languageEnglish
Pages (from-to)901-906
Number of pages6
JournalCurrent Biology
Volume25
Issue number7
DOIs
Publication statusPublished - 2015
Externally publishedYes

Fingerprint

DNA methylation
DNA Methylation
Germ Cells
Small Interfering RNA
germ cells
Cells
RNA
mice
Genes
testes
antisense RNA
proteins
retrotransposons
gene silencing
messenger RNA
spermatogenesis
epigenetics
Testis
production technology
genes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Itou, D., Shiromoto, Y., Shin-ya, Y., Ishii, C., Nishimura, T., Ogonuki, N., ... Nakano, T. (2015). Induction of DNA methylation by artificial piRNA production in male germ cells. Current Biology, 25(7), 901-906. https://doi.org/10.1016/j.cub.2015.01.060

Induction of DNA methylation by artificial piRNA production in male germ cells. / Itou, Daisuke; Shiromoto, Yusuke; Shin-ya, Yukiho; Ishii, Chika; Nishimura, Toru; Ogonuki, Narumi; Ogura, Atsuo; Hasuwa, Hidetoshi; Fujihara, Yoshitaka; Kuramochi-Miyagawa, Satomi; Nakano, Toru.

In: Current Biology, Vol. 25, No. 7, 2015, p. 901-906.

Research output: Contribution to journalArticle

Itou, D, Shiromoto, Y, Shin-ya, Y, Ishii, C, Nishimura, T, Ogonuki, N, Ogura, A, Hasuwa, H, Fujihara, Y, Kuramochi-Miyagawa, S & Nakano, T 2015, 'Induction of DNA methylation by artificial piRNA production in male germ cells', Current Biology, vol. 25, no. 7, pp. 901-906. https://doi.org/10.1016/j.cub.2015.01.060
Itou D, Shiromoto Y, Shin-ya Y, Ishii C, Nishimura T, Ogonuki N et al. Induction of DNA methylation by artificial piRNA production in male germ cells. Current Biology. 2015;25(7):901-906. https://doi.org/10.1016/j.cub.2015.01.060
Itou, Daisuke ; Shiromoto, Yusuke ; Shin-ya, Yukiho ; Ishii, Chika ; Nishimura, Toru ; Ogonuki, Narumi ; Ogura, Atsuo ; Hasuwa, Hidetoshi ; Fujihara, Yoshitaka ; Kuramochi-Miyagawa, Satomi ; Nakano, Toru. / Induction of DNA methylation by artificial piRNA production in male germ cells. In: Current Biology. 2015 ; Vol. 25, No. 7. pp. 901-906.
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