Induction of mexCD-oprJ operon for a multidrug efflux pump by disinfectants in wild-type Pseudomonas aeruginosa PAO1

Yuji Morita, Takeshi Murata, Takehiko Mima, Sumiko Shiota, Teruo Kuroda, Tohru Mizushima, Naomasa Gotoh, Takeshi Nishino, Tomofusa Tsuchiya

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)

Abstract

Induction of the MexCD-OprJ multidrug efflux pump was investigated in wild-type Pseudomonas aeruginosa PAO1. MexCD-OprJ was induced by clinically important disinfectants such as benzalkonium chloride and chlorhexidine gluconate, and by some cytotoxic agents such as tetraphenylphosphonium chloride, ethidium bromide and rhodamine 6G. MexCD-OprJ was not induced by norfloxacin, tetracycline, chloramphenicol, streptomycin, erythromycin or carbenicillin, although they are substrates for the pump. Cells of PAO1 showed increased resistance to norfloxacin when grown in the presence of the inducers of the mexCD-oprJ operon mentioned above. These results indicate that MexCD-OprJ plays an important role in intrinsic multidrug resistance in wild-type P. aeruginosa in hospitals where disinfectants are used frequently.

Original languageEnglish
Pages (from-to)991-994
Number of pages4
JournalJournal of Antimicrobial Chemotherapy
Volume51
Issue number4
DOIs
Publication statusPublished - 2003 Apr 1

Keywords

  • Inducers
  • MexCD-OprJ
  • Multidrug efflux pump
  • P. aeruginosa

ASJC Scopus subject areas

  • Pharmacology
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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