Induction of neurite outgrowth of PC12 cells by an inhibitor of vacuolar H+-ATPase, bafilomycin A1

Hiroomi Tamura, Shoji Ohkuma

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Bafilomycin A1, a selective inhibitor of vacuolar H+-ATPase, induced neurite outgrowth of PC12 cells dose- and time-dependently: more than 50% of the cells extended neurite-like spikes after 24 h treatment with 100 nM bafilomycin A1. Its dose-response ran roughly parallel to that of a bafilomycin A1-induced lysosomal pH increase. It was inhibited by LiCl, an inhibitor of the phosphorylation of microtubule-associated proteins and, like nerve growth factor (NGF)-induced neurite outgrowth, it was also inhibited by cycloheximide and actinomycin D. But, unlike the NGF-effect, it was not associated with rapid induction of c-fos.

Original languageEnglish
Pages (from-to)51-55
Number of pages5
JournalFEBS Letters
Volume294
Issue number1-2
DOIs
Publication statusPublished - 1991 Dec 2
Externally publishedYes

Fingerprint

Vacuolar Proton-Translocating ATPases
PC12 Cells
Nerve Growth Factor
Phosphorylation
Microtubule-Associated Proteins
Dactinomycin
Neurites
Cycloheximide
bafilomycin A1
Neuronal Outgrowth

Keywords

  • Bafilomycin A
  • c-fos
  • Nerve growth factor
  • Neurite outgrowth
  • PC12 cell
  • Vacuolar H-ATPase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Induction of neurite outgrowth of PC12 cells by an inhibitor of vacuolar H+-ATPase, bafilomycin A1. / Tamura, Hiroomi; Ohkuma, Shoji.

In: FEBS Letters, Vol. 294, No. 1-2, 02.12.1991, p. 51-55.

Research output: Contribution to journalArticle

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