Induction of protective and therapeutic antitumor immunity by a DNA vaccine with a glioma antigen, SOX6

Ryo Ueda, Eiko Kinoshita, Rena Ito, Takeshi Kawase, Yutaka Kawakami, Masahiro Toda

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We previously reported identifying SOX6 as a glioma antigen by serological screening using a testis cDNA library. Its preferential expression and frequent IgG responses in glioma patients indicate that SOX6 may be a useful target for immunotherapy. To examine whether cytotoxic T-lymphocyte (CTL) responses specific for SOX6 to destroy glioma can be generated in vivo, we treated glioma-bearing mice by vaccination with a plasmid DNA encoding murine full-length SOX6 protein. Following SOX6-DNA vaccination, CTLs specific for SOX6-expressing glioma cells were induced, while normal autologous-cells that had restrictedly expressed SOX6 during embryogenesis were not destroyed. Furthermore, DNA vaccination with SOX6 exerted protective and therapeutic antitumor responses in the glioma-bearing mice. This antitumor activity was abrogated by the depletion of CD4 positive T cells and/or CD8 positive T cells. These results suggest that the SOX6 protein has multiple CTL and helper epitopes to induce antitumor activity and the effectiveness of SOX6-DNA vaccine for the prevention and treatment of glioma.

Original languageEnglish
Pages (from-to)2274-2279
Number of pages6
JournalInternational Journal of Cancer
Volume122
Issue number10
DOIs
Publication statusPublished - 2008 May 15

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Keywords

  • Antitumor immunity
  • CTL response
  • DNA vaccine
  • Glioma antigen
  • SOX6

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

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