Induction of sesquiterpenoid production by methyl jasmonate in aquilaria sinensis cell suspension culture

Michiho Ito, Ken Ichiro Okimoto, Toru Yagura, Gisho Honda, Fumiyuki Kiuchi, Yasuo Shimada

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

A suspension cell culture was established from Aquilaria sinensis, a timber species producing fragrant woody material called "Agarwood." Methyl jasmonate was added to this culture in order to induce production of fragrant compounds. Cells were harvested after seven days of incubation and subjected to solid phase micro-extraction to produce an extract that was analyzed by GC/MS. Three peaks appeared on GC and were identified as α-guaiene, δ-guaiene (= 1(10),11-guaiadiene), and α-humulene based on their mass fragmentation patterns and 13C-NMR data. The production of the guaiene derivatives and α-humulene showed different kinetics, which may mean they are the products of different induced biosynthetic enzymes.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalJournal of Essential Oil Research
Volume17
Issue number2
Publication statusPublished - 2005 Mar
Externally publishedYes

Fingerprint

Aquilaria sinensis
Thymelaeaceae
humulene
methyl jasmonate
Cell culture
sesquiterpenoids
cell suspension culture
Suspensions
Cell Culture Techniques
Solid Phase Extraction
Timber
chemical derivatives
Nuclear magnetic resonance
Derivatives
kinetics
Kinetics
extracts
Enzymes
enzymes
cells

Keywords

  • Agarwood
  • Aquilaria sinensis
  • Cell culture
  • Methyl jasmonate
  • Sesquiterpenoid production
  • Solid phase micro-extraction
  • Thymelaeaceae

ASJC Scopus subject areas

  • Food Science
  • Chemistry (miscellaneous)

Cite this

Induction of sesquiterpenoid production by methyl jasmonate in aquilaria sinensis cell suspension culture. / Ito, Michiho; Okimoto, Ken Ichiro; Yagura, Toru; Honda, Gisho; Kiuchi, Fumiyuki; Shimada, Yasuo.

In: Journal of Essential Oil Research, Vol. 17, No. 2, 03.2005, p. 175-180.

Research output: Contribution to journalArticle

Ito, Michiho ; Okimoto, Ken Ichiro ; Yagura, Toru ; Honda, Gisho ; Kiuchi, Fumiyuki ; Shimada, Yasuo. / Induction of sesquiterpenoid production by methyl jasmonate in aquilaria sinensis cell suspension culture. In: Journal of Essential Oil Research. 2005 ; Vol. 17, No. 2. pp. 175-180.
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