Induction of sesquiterpenoid production by methyl jasmonate in aquilaria sinensis cell suspension culture

Michiho Ito, Ken ichiro Okimoto, Toru Yagura, Gisho Honda, Fumiyuki Kiuchi, Yasuo Shimada

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40 Citations (Scopus)


A suspension cell culture was established from Aquilaria sinensis, a timber species producing fragrant woody material called “Agarwood.” Methyl jasmonate was added to this culture in order to induce production of fragrant compounds. Cells were harvested after seven days of incubation and subjected to solid phase micro-extraction to produce an extract that was analyzed by GC/MS. Three peaks appeared on GC and were identifi ed as α-guaiene, γ-guaiene (= 1(10),11-guaiadiene), and α-humulene based on their mass fragmentation patterns and13C-NMR data. The production of the guaiene derivatives and α-humulene showed different kinetics, which may mean they are the products of different induced biosynthetic enzymes.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalJournal of Essential Oil Research
Issue number2
Publication statusPublished - 2005 Jan 1
Externally publishedYes



  • Agarwood
  • Aquilaria sinensis
  • Cell culture
  • Methyl jasmonate
  • Ses-quiterpenoid production
  • Solid phase micro-extraction
  • Thymelaeaceae

ASJC Scopus subject areas

  • Chemistry(all)

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