Induction of Th17 cells by segmented filamentous bacteria in the murine intestine

Adam M. Farkas, Casandra Panea, Yoshiyuki Goto, Gaku Nakato, Marta Galan-Diez, Seiko Narushima, Kenya Honda, Ivaylo I. Ivanov

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Segmented filamentous bacteria (SFB) are Gram-positive, anaerobic, spore-forming commensals that reside in the gut of many animal species. Described more than forty years ago, SFB have recently gained interest due to their unique ability to modulate the host immune system through induction of IgA and Th17 cells. Here, we describe a collection of methods to detect and quantify SFB and SFB adhesion in intestinal mucosa, as well as SFB-specific CD4 T cells in the lamina propria. In addition, we describe methods for purification of SFB from fecal material of SFB-monoassociated gnotobiotic mice. Using these methods we examine the kinetics of SFB colonization and Th17 cell induction. We also show that SFB colonize unevenly the intestinal mucosa and that SFB adherence occurs predominantly in the terminal ileum and correlates with an increased proportion of SFB-specific Th17 cells.

Original languageEnglish
Pages (from-to)104-111
Number of pages8
JournalJournal of Immunological Methods
Volume421
DOIs
Publication statusPublished - 2015

Fingerprint

Th17 Cells
Intestines
Bacteria
Intestinal Mucosa
Germ-Free Life
Gram-Positive Bacteria
Spores
Ileum
Immunoglobulin A
Immune System
Mucous Membrane
T-Lymphocytes

Keywords

  • CD4 T cells
  • Commensal microbiota
  • Intestinal epithelial cells
  • Mucosa-associated bacteria
  • Segmented filamentous bacteria
  • SFB
  • Th17 cells

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Farkas, A. M., Panea, C., Goto, Y., Nakato, G., Galan-Diez, M., Narushima, S., ... Ivanov, I. I. (2015). Induction of Th17 cells by segmented filamentous bacteria in the murine intestine. Journal of Immunological Methods, 421, 104-111. https://doi.org/10.1016/j.jim.2015.03.020

Induction of Th17 cells by segmented filamentous bacteria in the murine intestine. / Farkas, Adam M.; Panea, Casandra; Goto, Yoshiyuki; Nakato, Gaku; Galan-Diez, Marta; Narushima, Seiko; Honda, Kenya; Ivanov, Ivaylo I.

In: Journal of Immunological Methods, Vol. 421, 2015, p. 104-111.

Research output: Contribution to journalArticle

Farkas, AM, Panea, C, Goto, Y, Nakato, G, Galan-Diez, M, Narushima, S, Honda, K & Ivanov, II 2015, 'Induction of Th17 cells by segmented filamentous bacteria in the murine intestine', Journal of Immunological Methods, vol. 421, pp. 104-111. https://doi.org/10.1016/j.jim.2015.03.020
Farkas, Adam M. ; Panea, Casandra ; Goto, Yoshiyuki ; Nakato, Gaku ; Galan-Diez, Marta ; Narushima, Seiko ; Honda, Kenya ; Ivanov, Ivaylo I. / Induction of Th17 cells by segmented filamentous bacteria in the murine intestine. In: Journal of Immunological Methods. 2015 ; Vol. 421. pp. 104-111.
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