Induction of thioredoxin, a redox-active protein, by ovarian steroid hormones during growth and differentiation of endometrial stromal cells in vitro

Tetsuo Maruyama, Yoshifumi Sachi, Keizo Furuke, Yuki Kitaoka, Hideharu Kanzaki, Yasunori Yoshimura, Junji Yodoi

Research output: Contribution to journalArticle

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Abstract

Human thioredoxin (hTrx) is a cellular redox-active protein that catalyzes dithiol/disulfide exchange reactions, thus controlling multiple biological functions, including cell growth-promoting activity. Here we show that the expression of hTrx protein and messenger RNA was up-regulated by incubation with 17β-estradiol (E2) in primary culture of stromal cells isolated from human endometrium. Maximal enhancement of hTrx protein and messenger RNA was observed after 6-12 h of incubation with 10-100 nM E2, and the enhancing effect was suppressed by tamoxifen, an estrogen antagonist. Release of hTrx into the culture medium was markedly augmented after 5-day exposure of E2 plus progesterone (P) accompanied by in vitro differentiation of endometrial stromal cells (decidualization). Immunocytochemical studies showed that hTrx was localized in the nucleus, nucleolus, and cytosol in the stromal cells. Strongly enhanced immunoreactivity for hTrx was observed in the E2-treated cells, whereas there was no apparent difference in the pattern of subcellular localization among the untreated and E2- and/or P- treated cells. Although 1-50 μg/ml recombinant hTrx alone did not promote endometrial stromal cell growth, epidermal growth factor-dependent mitogenesis was additively enhanced by hTrx. Our results indicate that hTrx modulates endometrial cell growth, acting as a comitogenic factor for epidermal growth factor, which is known to be a mediator of estrogen action. It is also suggested that hTrx is deeply involved in the hormonal control of the endometrium by E2 and P, playing a regulatory role in endometrial cell growth and differentiation.

Original languageEnglish
Pages (from-to)365-372
Number of pages8
JournalEndocrinology
Volume140
Issue number1
DOIs
Publication statusPublished - 1999

Fingerprint

Thioredoxins
Stromal Cells
Growth Hormone
Oxidation-Reduction
Steroids
Proteins
Endometrium
Epidermal Growth Factor
Growth
Estrogen Antagonists
In Vitro Techniques
Messenger RNA
Tamoxifen
Disulfides
Cytosol
Progesterone
Culture Media
Cell Differentiation
Estradiol
Estrogens

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Induction of thioredoxin, a redox-active protein, by ovarian steroid hormones during growth and differentiation of endometrial stromal cells in vitro. / Maruyama, Tetsuo; Sachi, Yoshifumi; Furuke, Keizo; Kitaoka, Yuki; Kanzaki, Hideharu; Yoshimura, Yasunori; Yodoi, Junji.

In: Endocrinology, Vol. 140, No. 1, 1999, p. 365-372.

Research output: Contribution to journalArticle

Maruyama, Tetsuo ; Sachi, Yoshifumi ; Furuke, Keizo ; Kitaoka, Yuki ; Kanzaki, Hideharu ; Yoshimura, Yasunori ; Yodoi, Junji. / Induction of thioredoxin, a redox-active protein, by ovarian steroid hormones during growth and differentiation of endometrial stromal cells in vitro. In: Endocrinology. 1999 ; Vol. 140, No. 1. pp. 365-372.
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