Inefficient phagosome maturation in infant macrophages

Fumiji Saito, Hirotaka Kuwata, Eiji Oiki, Masato Koike, Yasuo Uchiyama, Kenya Honda, Kiyoshi Takeda

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The quantitative and qualitative differences between the immune systems of infants and adults have been extensively investigated in the context of adaptive immunity. Here, we demonstrate that the infantile innate immune system is immature and weak against bacterial infections. Upon infection by Escherichia coli, macrophages from infantile mice showed a lower performance in killing the bacteria. In infant macrophages, bacteria were taken up relatively normally and delivered into lysosomal compartments, but not efficiently digested. The inefficient bacterial killing in infant macrophages was correlated with impaired acidification of the lysosomal compartments and reduced lysosomal recruitment of Rab7, an essential component of the acidification process. The acidification defect was not intrinsic to the cells, and was rescued by pretreatment with interferon-γ. Thus, we propose that the limited capacity of phagosome maturation is one of the major causes of the high sensitivity to infectious microorganisms during infancy and that the specific cytokine milieu shapes the nature of infantile innate immunity.

Original languageEnglish
Pages (from-to)113-118
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume375
Issue number1
DOIs
Publication statusPublished - 2008 Oct 10
Externally publishedYes

Fingerprint

Phagosomes
Acidification
Macrophages
Immune system
Immune System
Bacteria
Escherichia coli Infections
Adaptive Immunity
Bacterial Infections
Innate Immunity
Microorganisms
Escherichia coli
Interferons
Cytokines
Defects

Keywords

  • Bacterial infection
  • Infant
  • Interferon-γ
  • Macrophage
  • Phagosome
  • Rab7

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Inefficient phagosome maturation in infant macrophages. / Saito, Fumiji; Kuwata, Hirotaka; Oiki, Eiji; Koike, Masato; Uchiyama, Yasuo; Honda, Kenya; Takeda, Kiyoshi.

In: Biochemical and Biophysical Research Communications, Vol. 375, No. 1, 10.10.2008, p. 113-118.

Research output: Contribution to journalArticle

Saito, F, Kuwata, H, Oiki, E, Koike, M, Uchiyama, Y, Honda, K & Takeda, K 2008, 'Inefficient phagosome maturation in infant macrophages', Biochemical and Biophysical Research Communications, vol. 375, no. 1, pp. 113-118. https://doi.org/10.1016/j.bbrc.2008.07.141
Saito, Fumiji ; Kuwata, Hirotaka ; Oiki, Eiji ; Koike, Masato ; Uchiyama, Yasuo ; Honda, Kenya ; Takeda, Kiyoshi. / Inefficient phagosome maturation in infant macrophages. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 375, No. 1. pp. 113-118.
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