Inefficient transcription of the myelin basic protein gene possibly causes hypomyelination in myelin-deficient mutant mice

Hideyuki Okano, M. Miura, A. Moriguchi, K. Ikenaka, Y. Tsukada, K. Mikoshiba

Research output: Contribution to journalArticle

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Abstract

A hereditary dysmyelination mutation, named myelin deficient (mld), is considered to be allelic to shiverer, a deletion mutation of the myelin basic protein (MBP) gene. The present study showed that MBP expression is greatly reduced in mld, but that it is still detectable. Northern blot analysis revealed that the pronounced decrease in the MBP level in mld resulted from a reduced mRNA level and was not caused by deletion of a large portion of the MBP gene as in shiverer. Southern blod studies with BamHI-digested chromosomal DNA suggested some part of the MBP gene, at least the 5'-portion, was duplicated in mld. These results indicated that the mld and shiverer mutations were different from each other, even though genetic allelism between the two was reconfirmed. We also examined the developmental pattern of the gene expression of MBP and that of another protein, myelin proteolipid protein (PLP), specifically expressed in the oligodendrocyte, in mld by RNA dot blot study. The mRNA level of MBP in mld was greatly reduced during the active myelination stages, gradually increasing and remaining constant in the later stages. The PLP-mRNA content in mld was almost normal (60-80% that of control) at any stage of development. All these findings imply that the primary defect in mld is due to reduced transcriptional activity of the MBP gene.

Original languageEnglish
Pages (from-to)470-476
Number of pages7
JournalJournal of Neurochemistry
Volume48
Issue number2
DOIs
Publication statusPublished - 1987
Externally publishedYes

Fingerprint

Myelin Basic Protein
Transcription
Myelin Sheath
Genes
Messenger RNA
Myelin Proteolipid Protein
Proteolipids
Developmental Genes
Mutation
Sequence Deletion
Oligodendroglia
Gene expression
Northern Blotting
Proteins
RNA
Defects
Gene Expression
DNA

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Inefficient transcription of the myelin basic protein gene possibly causes hypomyelination in myelin-deficient mutant mice. / Okano, Hideyuki; Miura, M.; Moriguchi, A.; Ikenaka, K.; Tsukada, Y.; Mikoshiba, K.

In: Journal of Neurochemistry, Vol. 48, No. 2, 1987, p. 470-476.

Research output: Contribution to journalArticle

Okano, Hideyuki ; Miura, M. ; Moriguchi, A. ; Ikenaka, K. ; Tsukada, Y. ; Mikoshiba, K. / Inefficient transcription of the myelin basic protein gene possibly causes hypomyelination in myelin-deficient mutant mice. In: Journal of Neurochemistry. 1987 ; Vol. 48, No. 2. pp. 470-476.
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