Influence of a skull cranial window on the measurement of haemoglobin concentration in cortical tissue by multi-spectral imaging analysis

Koichiro Sakaguchi, Shunsuke Furukawa, Satoshi Matsuo, Naotaka Sakashita, Takushige Katsura, Kyoko Yamazaki, Hideo Kawaguchi, Atsushi Maki, Eiji Okada

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    The simplified cranial window, which is transparent thinned skull, has been used for the optical imaging of cortical tissue of small animals to measure the concentration change in haemoglobin as an intrinsic signal of brain activation. The multi-spectral images of the cortical tissue of guinea pigs through the skull cranial window were compared with those of the exposed cortex to evaluate the influence of the scattering and absorption properties of the skull on the measurement of the concentration change in haemoglobin. Although skull thickness affects the sensitivity of the optical signal due to a decrease in mean optical path length in the cortical tissue, the influence of the skull cranial window on the wavelength dependence of optical path length can be ignored when the skull thickness is less than approximately 100 mm. Accurate concentration changes in oxygenated and deoxygenated haemoglobins can be calculated from the optical signal measured through a skull cranial window and the wavelength dependence of optical path length for the exposed cortex.

    Original languageEnglish
    Pages (from-to)74-80
    Number of pages7
    JournalOptical Review
    Volume16
    Issue number2
    DOIs
    Publication statusPublished - 2009 Mar 1

    Keywords

    • Biomedical optics
    • Brain activity
    • Cranial window
    • Multi-spectral imaging
    • Optical intrinsic signal
    • Optical path length
    • Thinned skull

    ASJC Scopus subject areas

    • Atomic and Molecular Physics, and Optics

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