Abstract
Purpose: Platelets containing a number of growth factors (platelet-derived growth factor [PDGF], transforming growth factor-β [TGF-β], etc) can be obtained in high concentrations through centrifugal separation and are used in clinical applications as platelet-rich plasma (PRP). However, only a few studies have been conducted on the growth factors present in PRP. In this study, we focused on the concentrations of growth factors in PRP and clarified the influence of using antiplatelet substances in the process of platelet concentration to improve the concentration rate of growth factors in PRP. Materials and Methods: We made platelet pellets from whole blood obtained from humans with or without some antiplatelet substances (prostaglandin E 1, aspirin, apyrase). Platelet pellets were resuspended in phosphate-buffered saline as platelet resuspensions. We measured PDGF and TGF-β1 concentrations in the samples. In measurements, we had the samples treated to release growth factors from platelets to measure accurate concentrations. Results: PDGF and TGF-β1 were concentrated to a mean of over 400% in the samples with antiplatelet substances as compared with the samples without antiplatelet substances. Conclusions: The antiplatelet substances were effective for efficiently concentrating growth factors in platelets.
| Original language | English |
|---|---|
| Pages (from-to) | 714-718 |
| Number of pages | 5 |
| Journal | Journal of Oral and Maxillofacial Surgery |
| Volume | 62 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2004 Jun |
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ASJC Scopus subject areas
- Dentistry(all)
- Surgery
Cite this
Influence of antiplatelet substances on platelet-rich plasma. / Yazawa, Masaki; Ogata, Hisao; Nakajima, Tatsuo; Watanabe, Naohide.
In: Journal of Oral and Maxillofacial Surgery, Vol. 62, No. 6, 06.2004, p. 714-718.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Influence of antiplatelet substances on platelet-rich plasma
AU - Yazawa, Masaki
AU - Ogata, Hisao
AU - Nakajima, Tatsuo
AU - Watanabe, Naohide
PY - 2004/6
Y1 - 2004/6
N2 - Purpose: Platelets containing a number of growth factors (platelet-derived growth factor [PDGF], transforming growth factor-β [TGF-β], etc) can be obtained in high concentrations through centrifugal separation and are used in clinical applications as platelet-rich plasma (PRP). However, only a few studies have been conducted on the growth factors present in PRP. In this study, we focused on the concentrations of growth factors in PRP and clarified the influence of using antiplatelet substances in the process of platelet concentration to improve the concentration rate of growth factors in PRP. Materials and Methods: We made platelet pellets from whole blood obtained from humans with or without some antiplatelet substances (prostaglandin E 1, aspirin, apyrase). Platelet pellets were resuspended in phosphate-buffered saline as platelet resuspensions. We measured PDGF and TGF-β1 concentrations in the samples. In measurements, we had the samples treated to release growth factors from platelets to measure accurate concentrations. Results: PDGF and TGF-β1 were concentrated to a mean of over 400% in the samples with antiplatelet substances as compared with the samples without antiplatelet substances. Conclusions: The antiplatelet substances were effective for efficiently concentrating growth factors in platelets.
AB - Purpose: Platelets containing a number of growth factors (platelet-derived growth factor [PDGF], transforming growth factor-β [TGF-β], etc) can be obtained in high concentrations through centrifugal separation and are used in clinical applications as platelet-rich plasma (PRP). However, only a few studies have been conducted on the growth factors present in PRP. In this study, we focused on the concentrations of growth factors in PRP and clarified the influence of using antiplatelet substances in the process of platelet concentration to improve the concentration rate of growth factors in PRP. Materials and Methods: We made platelet pellets from whole blood obtained from humans with or without some antiplatelet substances (prostaglandin E 1, aspirin, apyrase). Platelet pellets were resuspended in phosphate-buffered saline as platelet resuspensions. We measured PDGF and TGF-β1 concentrations in the samples. In measurements, we had the samples treated to release growth factors from platelets to measure accurate concentrations. Results: PDGF and TGF-β1 were concentrated to a mean of over 400% in the samples with antiplatelet substances as compared with the samples without antiplatelet substances. Conclusions: The antiplatelet substances were effective for efficiently concentrating growth factors in platelets.
UR - http://www.scopus.com/inward/record.url?scp=2942530741&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2942530741&partnerID=8YFLogxK
U2 - 10.1016/j.joms.2004.01.008
DO - 10.1016/j.joms.2004.01.008
M3 - Article
C2 - 15170284
AN - SCOPUS:2942530741
VL - 62
SP - 714
EP - 718
JO - Journal of Oral and Maxillofacial Surgery
JF - Journal of Oral and Maxillofacial Surgery
SN - 0278-2391
IS - 6
ER -