Influence of calmodulin antagonists on Ca2+ transport in the lens

A. Iimuro, M. Takehana, S. Iwata

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Attempts were made to elucidate the relation between lens opacification resulting from inhibition of Ca2+ transport and high calcium accumulation in the lens. Mouse lenses were incubated with naphthalenesulfonamide compounds (W-5, W-7, W-12 and W-13) which act as calmodulin antagonists. When lens Ca2+-ATPase activity was inhibited by those calmodulin antagonists, an accumulation of calcium in the lens was observed. This phenomenon depended on the chemical structure of calmodulin antagonist. In lenses treated with W-7, more than 97% of excess calcium was bound. Insoluble protein increased from 8.5 to 17% of the total protein and the degree of lens opacification increased to 85.1%. These results suggested that dysfunction of the Ca-pump system lead to calcium accumulation, thus in turn inducing protein denaturation which in turn is followed by lens opacification.

Original languageEnglish
Pages (from-to)95-100
Number of pages6
JournalOphthalmic Research
Volume19
Issue number2
Publication statusPublished - 1987

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Calmodulin
Lenses
Calcium
Protein Denaturation
Crystallins
Calcium-Transporting ATPases
Proteins

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Iimuro, A., Takehana, M., & Iwata, S. (1987). Influence of calmodulin antagonists on Ca2+ transport in the lens. Ophthalmic Research, 19(2), 95-100.

Influence of calmodulin antagonists on Ca2+ transport in the lens. / Iimuro, A.; Takehana, M.; Iwata, S.

In: Ophthalmic Research, Vol. 19, No. 2, 1987, p. 95-100.

Research output: Contribution to journalArticle

Iimuro, A, Takehana, M & Iwata, S 1987, 'Influence of calmodulin antagonists on Ca2+ transport in the lens', Ophthalmic Research, vol. 19, no. 2, pp. 95-100.
Iimuro, A. ; Takehana, M. ; Iwata, S. / Influence of calmodulin antagonists on Ca2+ transport in the lens. In: Ophthalmic Research. 1987 ; Vol. 19, No. 2. pp. 95-100.
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