Influence of chemical modification of Nα-cocoyl-L-arginine ethyl ester on its hepatitis B surface antigen-inactivating effect

Yoshikazu Sugimoto, S. Toyoshima

Research output: Contribution to journalArticle

Abstract

We have reported previously that N(α)-cocoyl-L-arginine ethyl ester (CAE) strongly inactivates hepatitis B surface antigen. Replacement of the L-arginine moiety of CAE by L-lysine did not decrease the HBsAg-inactivating effect of CAE, whereas replacement by some neutral amino acids and L-ornithine decreased it. Esterification of the carboxyl group of N(α)-acyl-L-arginine enhanced its inactivating effect. When the ethyl ester of CAE was converted to an amide group, the effect was appreciably decreased. Modification of the carboxyl group was essential for the inactivation. The effectiveness of N(α)-acyl-L-arginine ethyl ester depends upon the length of the acyl group, with the optimum length for the inactivation of HBsAg being C12 to C14. In addition to CAE, N(α)-lauroyl-L-lysine ethyl ester and N(α)-cocoyl-L-arginine amide were found to be strong inactivators of HBsAg. Significant inactivating effects on HBsAg were not observed in many anionic detergents containing an amino acid. These results suggest that for strongly inactivating HBsAg, a compound should contain a special amino acid, such as L-arginine, and a long acyl group and exhibit a cationic property.

Original languageEnglish
Pages (from-to)525-528
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume18
Issue number4
Publication statusPublished - 1980

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Hepatitis B Surface Antigens
Arginine
Amides
Lysine
Esters
arginine ethyl ester
Neutral Amino Acids
Amino Acids
Ornithine
Esterification
Detergents

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

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abstract = "We have reported previously that N(α)-cocoyl-L-arginine ethyl ester (CAE) strongly inactivates hepatitis B surface antigen. Replacement of the L-arginine moiety of CAE by L-lysine did not decrease the HBsAg-inactivating effect of CAE, whereas replacement by some neutral amino acids and L-ornithine decreased it. Esterification of the carboxyl group of N(α)-acyl-L-arginine enhanced its inactivating effect. When the ethyl ester of CAE was converted to an amide group, the effect was appreciably decreased. Modification of the carboxyl group was essential for the inactivation. The effectiveness of N(α)-acyl-L-arginine ethyl ester depends upon the length of the acyl group, with the optimum length for the inactivation of HBsAg being C12 to C14. In addition to CAE, N(α)-lauroyl-L-lysine ethyl ester and N(α)-cocoyl-L-arginine amide were found to be strong inactivators of HBsAg. Significant inactivating effects on HBsAg were not observed in many anionic detergents containing an amino acid. These results suggest that for strongly inactivating HBsAg, a compound should contain a special amino acid, such as L-arginine, and a long acyl group and exhibit a cationic property.",
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AU - Sugimoto, Yoshikazu

AU - Toyoshima, S.

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N2 - We have reported previously that N(α)-cocoyl-L-arginine ethyl ester (CAE) strongly inactivates hepatitis B surface antigen. Replacement of the L-arginine moiety of CAE by L-lysine did not decrease the HBsAg-inactivating effect of CAE, whereas replacement by some neutral amino acids and L-ornithine decreased it. Esterification of the carboxyl group of N(α)-acyl-L-arginine enhanced its inactivating effect. When the ethyl ester of CAE was converted to an amide group, the effect was appreciably decreased. Modification of the carboxyl group was essential for the inactivation. The effectiveness of N(α)-acyl-L-arginine ethyl ester depends upon the length of the acyl group, with the optimum length for the inactivation of HBsAg being C12 to C14. In addition to CAE, N(α)-lauroyl-L-lysine ethyl ester and N(α)-cocoyl-L-arginine amide were found to be strong inactivators of HBsAg. Significant inactivating effects on HBsAg were not observed in many anionic detergents containing an amino acid. These results suggest that for strongly inactivating HBsAg, a compound should contain a special amino acid, such as L-arginine, and a long acyl group and exhibit a cationic property.

AB - We have reported previously that N(α)-cocoyl-L-arginine ethyl ester (CAE) strongly inactivates hepatitis B surface antigen. Replacement of the L-arginine moiety of CAE by L-lysine did not decrease the HBsAg-inactivating effect of CAE, whereas replacement by some neutral amino acids and L-ornithine decreased it. Esterification of the carboxyl group of N(α)-acyl-L-arginine enhanced its inactivating effect. When the ethyl ester of CAE was converted to an amide group, the effect was appreciably decreased. Modification of the carboxyl group was essential for the inactivation. The effectiveness of N(α)-acyl-L-arginine ethyl ester depends upon the length of the acyl group, with the optimum length for the inactivation of HBsAg being C12 to C14. In addition to CAE, N(α)-lauroyl-L-lysine ethyl ester and N(α)-cocoyl-L-arginine amide were found to be strong inactivators of HBsAg. Significant inactivating effects on HBsAg were not observed in many anionic detergents containing an amino acid. These results suggest that for strongly inactivating HBsAg, a compound should contain a special amino acid, such as L-arginine, and a long acyl group and exhibit a cationic property.

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