Influence of cluster formation of acidic phospholipids on decrease in the affinity for ATP of DnaA protein

Tohru Mizushima, Yuichi Ishikawa, Eishi Obana, Masakazu Hase, Toshio Kubota, Tsutomu Katayama, Toyoki Kunitake, Eiji Watanabe, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

DnaA protein is the initiator of chromosomal DNA replication in Escherichia coli. We examined the influence of artificial mixed membrane composed of synthetic acidic (phosphate) lipid and basic (ammonium) lipid on the affinity of DnaA protein for ATP. Two sets of acidic and basic lipids with distinguishable numbers of hydrophobic alkyl chains were devised. Synthetic membranes made of the sole acidic lipid but not the basic bilayers inhibited the ATP binding to DnaA protein and stimulated the release of ATP from the ATP-DnaA complex. The basic bilayer-forming compounds served as the matrix for the guest acidic lipids. Acidic lipids dispersed in the basic matrix membrane had little effect on ATP binding and on ATP release. Conversely, acidic lipids forming cluster structures in the mixed artificial membranes inhibited the ATP binding and stimulated the release of ATP. These observations suggest that in mixed lipid bilayers, a cluster structure of acidic lipids seems to be an important parameter to decrease the affinity of DnaA protein for ATP.

Original languageEnglish
Pages (from-to)3633-3638
Number of pages6
JournalJournal of Biological Chemistry
Volume271
Issue number7
DOIs
Publication statusPublished - 1996 Feb 16
Externally publishedYes

Fingerprint

Phospholipids
Adenosine Triphosphate
Lipids
Proteins
Artificial Membranes
Membranes
Lipid bilayers
Lipid Bilayers
DNA Replication
Ammonium Compounds
Escherichia coli
Phosphates
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Mizushima, T., Ishikawa, Y., Obana, E., Hase, M., Kubota, T., Katayama, T., ... Sekimizu, K. (1996). Influence of cluster formation of acidic phospholipids on decrease in the affinity for ATP of DnaA protein. Journal of Biological Chemistry, 271(7), 3633-3638. https://doi.org/10.1074/jbc.271.7.3633

Influence of cluster formation of acidic phospholipids on decrease in the affinity for ATP of DnaA protein. / Mizushima, Tohru; Ishikawa, Yuichi; Obana, Eishi; Hase, Masakazu; Kubota, Toshio; Katayama, Tsutomu; Kunitake, Toyoki; Watanabe, Eiji; Sekimizu, Kazuhisa.

In: Journal of Biological Chemistry, Vol. 271, No. 7, 16.02.1996, p. 3633-3638.

Research output: Contribution to journalArticle

Mizushima, T, Ishikawa, Y, Obana, E, Hase, M, Kubota, T, Katayama, T, Kunitake, T, Watanabe, E & Sekimizu, K 1996, 'Influence of cluster formation of acidic phospholipids on decrease in the affinity for ATP of DnaA protein', Journal of Biological Chemistry, vol. 271, no. 7, pp. 3633-3638. https://doi.org/10.1074/jbc.271.7.3633
Mizushima, Tohru ; Ishikawa, Yuichi ; Obana, Eishi ; Hase, Masakazu ; Kubota, Toshio ; Katayama, Tsutomu ; Kunitake, Toyoki ; Watanabe, Eiji ; Sekimizu, Kazuhisa. / Influence of cluster formation of acidic phospholipids on decrease in the affinity for ATP of DnaA protein. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 7. pp. 3633-3638.
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