Influence of light intensity and repetition rate of nanosecond laser pulses on photodynamic therapy with PAD-S31 in mouse renal carcinoma cell line in vitro: Study for oxygen consumption and photobleaching

S. Kawauchi, Tsunenori Arai, K. Seguchi, Hiroshi Asanuma, S. Sato, M. Kikuchi, T. Takemura, I. Sakata, S. Nakajima

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

In order to determine the optimum light irradiation condition to treat deep lesions, we studied influence of light intensity and repetition rate of nanosecond light pulses on photodynamic therapy(PDT) with PAD-S31 (13,17-bis[1-carboxypropionyl]carbamoylethyl-3-ethenyl-8- ethoxyiminoethylidene-7-hydroxy-2,7,12,18-tetramethyl porphyrin sodium) to mouse renal carcinoma cell line(Renca) in vitro. The oxygen consumption and photobleaching were measured to explain this influence. We used the short light pulses(λ: 670 nm, FWHM: 5 ns) at the peak intensity of 0.6, 1.8 and 3.6 MW/cm2, repetition rate of 30 and 5 Hz, and used the total fluence of 40 J/cm2. We obtained over 80% cell growth inhibition rate at 0.6 MW/cm2 and 5 Hz. This irradiation condition was the lowest peak intensity and lowest repetition rate in our study. From the measurement for oxygen consumption in the well(culture medium) by a microelectrode and oxygen diffusion calculation by the finite element model, we predicted that the low repetition rate may supply sufficient oxygen for PDT by diffusion process. The photobleaching detection by fluorescence measurement showed that bleaching occurred more intensively at lower peak intensity i.e., the PDT process advanced in this intensity. With high peak intensity irradiation such that we used, it is supposed that the waste energy which was not absorbed by photosensitizer to suppress effective light dose for the PDT, since the number of photon per pulse was extremely larger than the number of the photosensitizer molecule in the cell. The PDT effect at our optimum irradiation condition (Over 80% cell growth inhibition rate) was higher than that by continuous wave irradiation at the same average power density and energy dose(40%). Therefore, we conclude that the irradiation condition of low peak intensity(0.6MW/cm2) and low repetition rate (5Hz) using the nanosecond pulsed irradiation was suitable for the PDT in cultured cell in vitro with PAD-S31.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsT.J. Dougherty
Pages138-143
Number of pages6
Volume4248
DOIs
Publication statusPublished - 2001
Externally publishedYes
EventOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X - San Jose, CA, United States
Duration: 2001 Jan 202001 Jan 21

Other

OtherOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X
CountryUnited States
CitySan Jose, CA
Period01/1/2001/1/21

Fingerprint

oxygen consumption
Photobleaching
Photodynamic therapy
cultured cells
luminous intensity
mice
Laser pulses
therapy
repetition
cancer
Cells
Irradiation
irradiation
Oxygen
pulses
lasers
Photosensitizers
Cell growth
Oxygen supply
dosage

Keywords

  • Light intensity
  • PAD-S31
  • Photodynamic therapy
  • Repetition rate

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Kawauchi, S., Arai, T., Seguchi, K., Asanuma, H., Sato, S., Kikuchi, M., ... Nakajima, S. (2001). Influence of light intensity and repetition rate of nanosecond laser pulses on photodynamic therapy with PAD-S31 in mouse renal carcinoma cell line in vitro: Study for oxygen consumption and photobleaching. In T. J. Dougherty (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4248, pp. 138-143) https://doi.org/10.1117/12.424445

Influence of light intensity and repetition rate of nanosecond laser pulses on photodynamic therapy with PAD-S31 in mouse renal carcinoma cell line in vitro : Study for oxygen consumption and photobleaching. / Kawauchi, S.; Arai, Tsunenori; Seguchi, K.; Asanuma, Hiroshi; Sato, S.; Kikuchi, M.; Takemura, T.; Sakata, I.; Nakajima, S.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / T.J. Dougherty. Vol. 4248 2001. p. 138-143.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kawauchi, S, Arai, T, Seguchi, K, Asanuma, H, Sato, S, Kikuchi, M, Takemura, T, Sakata, I & Nakajima, S 2001, Influence of light intensity and repetition rate of nanosecond laser pulses on photodynamic therapy with PAD-S31 in mouse renal carcinoma cell line in vitro: Study for oxygen consumption and photobleaching. in TJ Dougherty (ed.), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4248, pp. 138-143, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X, San Jose, CA, United States, 01/1/20. https://doi.org/10.1117/12.424445
Kawauchi S, Arai T, Seguchi K, Asanuma H, Sato S, Kikuchi M et al. Influence of light intensity and repetition rate of nanosecond laser pulses on photodynamic therapy with PAD-S31 in mouse renal carcinoma cell line in vitro: Study for oxygen consumption and photobleaching. In Dougherty TJ, editor, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4248. 2001. p. 138-143 https://doi.org/10.1117/12.424445
Kawauchi, S. ; Arai, Tsunenori ; Seguchi, K. ; Asanuma, Hiroshi ; Sato, S. ; Kikuchi, M. ; Takemura, T. ; Sakata, I. ; Nakajima, S. / Influence of light intensity and repetition rate of nanosecond laser pulses on photodynamic therapy with PAD-S31 in mouse renal carcinoma cell line in vitro : Study for oxygen consumption and photobleaching. Proceedings of SPIE - The International Society for Optical Engineering. editor / T.J. Dougherty. Vol. 4248 2001. pp. 138-143
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abstract = "In order to determine the optimum light irradiation condition to treat deep lesions, we studied influence of light intensity and repetition rate of nanosecond light pulses on photodynamic therapy(PDT) with PAD-S31 (13,17-bis[1-carboxypropionyl]carbamoylethyl-3-ethenyl-8- ethoxyiminoethylidene-7-hydroxy-2,7,12,18-tetramethyl porphyrin sodium) to mouse renal carcinoma cell line(Renca) in vitro. The oxygen consumption and photobleaching were measured to explain this influence. We used the short light pulses(λ: 670 nm, FWHM: 5 ns) at the peak intensity of 0.6, 1.8 and 3.6 MW/cm2, repetition rate of 30 and 5 Hz, and used the total fluence of 40 J/cm2. We obtained over 80{\%} cell growth inhibition rate at 0.6 MW/cm2 and 5 Hz. This irradiation condition was the lowest peak intensity and lowest repetition rate in our study. From the measurement for oxygen consumption in the well(culture medium) by a microelectrode and oxygen diffusion calculation by the finite element model, we predicted that the low repetition rate may supply sufficient oxygen for PDT by diffusion process. The photobleaching detection by fluorescence measurement showed that bleaching occurred more intensively at lower peak intensity i.e., the PDT process advanced in this intensity. With high peak intensity irradiation such that we used, it is supposed that the waste energy which was not absorbed by photosensitizer to suppress effective light dose for the PDT, since the number of photon per pulse was extremely larger than the number of the photosensitizer molecule in the cell. The PDT effect at our optimum irradiation condition (Over 80{\%} cell growth inhibition rate) was higher than that by continuous wave irradiation at the same average power density and energy dose(40{\%}). Therefore, we conclude that the irradiation condition of low peak intensity(0.6MW/cm2) and low repetition rate (5Hz) using the nanosecond pulsed irradiation was suitable for the PDT in cultured cell in vitro with PAD-S31.",
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N2 - In order to determine the optimum light irradiation condition to treat deep lesions, we studied influence of light intensity and repetition rate of nanosecond light pulses on photodynamic therapy(PDT) with PAD-S31 (13,17-bis[1-carboxypropionyl]carbamoylethyl-3-ethenyl-8- ethoxyiminoethylidene-7-hydroxy-2,7,12,18-tetramethyl porphyrin sodium) to mouse renal carcinoma cell line(Renca) in vitro. The oxygen consumption and photobleaching were measured to explain this influence. We used the short light pulses(λ: 670 nm, FWHM: 5 ns) at the peak intensity of 0.6, 1.8 and 3.6 MW/cm2, repetition rate of 30 and 5 Hz, and used the total fluence of 40 J/cm2. We obtained over 80% cell growth inhibition rate at 0.6 MW/cm2 and 5 Hz. This irradiation condition was the lowest peak intensity and lowest repetition rate in our study. From the measurement for oxygen consumption in the well(culture medium) by a microelectrode and oxygen diffusion calculation by the finite element model, we predicted that the low repetition rate may supply sufficient oxygen for PDT by diffusion process. The photobleaching detection by fluorescence measurement showed that bleaching occurred more intensively at lower peak intensity i.e., the PDT process advanced in this intensity. With high peak intensity irradiation such that we used, it is supposed that the waste energy which was not absorbed by photosensitizer to suppress effective light dose for the PDT, since the number of photon per pulse was extremely larger than the number of the photosensitizer molecule in the cell. The PDT effect at our optimum irradiation condition (Over 80% cell growth inhibition rate) was higher than that by continuous wave irradiation at the same average power density and energy dose(40%). Therefore, we conclude that the irradiation condition of low peak intensity(0.6MW/cm2) and low repetition rate (5Hz) using the nanosecond pulsed irradiation was suitable for the PDT in cultured cell in vitro with PAD-S31.

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