Influence of light irradiation on the development of mouse eggs in culture

Harum Itoh, Noriko Makimura, Tsunenori Arai, Hiroshi Tsubamoto, Kennichi Furuya, Makoto Kikuchi, Ichiro Nagata

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We investigated the influence of light environment on the development of mouse egg in vitro. The 1-cell mouse eggs were irradiated by various laser lights of which wavelengths were 632.8nm, 543nm, and 442nm in vitro. Actual light environment by means of the microscope light source in IVF-ET (in vitro) fertilization-embryo transfer) was measured to set the light dose in this experiment. Mouse eggs were cultured to observe cellular development stage. In case of 632.8nm radiation of 500KJ/m2 in dose (20 times larger than actual light environment), 97% of eggs stopped their division. In the case of 543nm and 442nm radiations no influences were observed in egg division.These results suggested that light environment might affect on the development of mouse egg in vitro.

Original languageEnglish
Title of host publicationProceedings of the Annual Conference on Engineering in Medicine and Biology
EditorsAndrew Y.J. Szeto, Rangaraj M. Rangayyan
PublisherPubl by IEEE
Pages1615-1616
Number of pages2
Volume15
Editionpt 3
ISBN (Print)0780313771
Publication statusPublished - 1993
Externally publishedYes
EventProceedings of the 15th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Part 3 (of 3) - San Diego, CA, USA
Duration: 1993 Oct 281993 Oct 31

Other

OtherProceedings of the 15th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Part 3 (of 3)
CitySan Diego, CA, USA
Period93/10/2893/10/31

Fingerprint

Irradiation
Dosimetry
Radiation
Light sources
Microscopes
Wavelength
Lasers
Experiments

ASJC Scopus subject areas

  • Bioengineering

Cite this

Itoh, H., Makimura, N., Arai, T., Tsubamoto, H., Furuya, K., Kikuchi, M., & Nagata, I. (1993). Influence of light irradiation on the development of mouse eggs in culture. In A. Y. J. Szeto, & R. M. Rangayyan (Eds.), Proceedings of the Annual Conference on Engineering in Medicine and Biology (pt 3 ed., Vol. 15, pp. 1615-1616). Publ by IEEE.

Influence of light irradiation on the development of mouse eggs in culture. / Itoh, Harum; Makimura, Noriko; Arai, Tsunenori; Tsubamoto, Hiroshi; Furuya, Kennichi; Kikuchi, Makoto; Nagata, Ichiro.

Proceedings of the Annual Conference on Engineering in Medicine and Biology. ed. / Andrew Y.J. Szeto; Rangaraj M. Rangayyan. Vol. 15 pt 3. ed. Publ by IEEE, 1993. p. 1615-1616.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Itoh, H, Makimura, N, Arai, T, Tsubamoto, H, Furuya, K, Kikuchi, M & Nagata, I 1993, Influence of light irradiation on the development of mouse eggs in culture. in AYJ Szeto & RM Rangayyan (eds), Proceedings of the Annual Conference on Engineering in Medicine and Biology. pt 3 edn, vol. 15, Publ by IEEE, pp. 1615-1616, Proceedings of the 15th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Part 3 (of 3), San Diego, CA, USA, 93/10/28.
Itoh H, Makimura N, Arai T, Tsubamoto H, Furuya K, Kikuchi M et al. Influence of light irradiation on the development of mouse eggs in culture. In Szeto AYJ, Rangayyan RM, editors, Proceedings of the Annual Conference on Engineering in Medicine and Biology. pt 3 ed. Vol. 15. Publ by IEEE. 1993. p. 1615-1616
Itoh, Harum ; Makimura, Noriko ; Arai, Tsunenori ; Tsubamoto, Hiroshi ; Furuya, Kennichi ; Kikuchi, Makoto ; Nagata, Ichiro. / Influence of light irradiation on the development of mouse eggs in culture. Proceedings of the Annual Conference on Engineering in Medicine and Biology. editor / Andrew Y.J. Szeto ; Rangaraj M. Rangayyan. Vol. 15 pt 3. ed. Publ by IEEE, 1993. pp. 1615-1616
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