Influence of light irradiation on the development of mouse eggs in culture

Harum Itoh; Noriko Makimura; Tsunenori Arai; Hiroshi Tsubamoto; Kennichi Furuya; Makoto Kikuchi; Ichiro Nagata

Influence of light irradiation on the development of mouse eggs in culture

Harum Itoh, Noriko Makimura, Tsunenori Arai, Hiroshi Tsubamoto, Kennichi Furuya, Makoto Kikuchi, Ichiro Nagata

Department of Applied Physics and Physico-Informatics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We investigated the influence of light environment on the development of mouse egg in vitro. The 1-cell mouse eggs were irradiated by various laser lights of which wavelengths were 632.8nm, 543nm, and 442nm in vitro. Actual light environment by means of the microscope light source in IVF-ET (in vitro) fertilization-embryo transfer) was measured to set the light dose in this experiment. Mouse eggs were cultured to observe cellular development stage. In case of 632.8nm radiation of 500KJ/m² in dose (20 times larger than actual light environment), 97% of eggs stopped their division. In the case of 543nm and 442nm radiations no influences were observed in egg division.These results suggested that light environment might affect on the development of mouse egg in vitro.

Original language	English
Title of host publication	Proceedings of the Annual Conference on Engineering in Medicine and Biology
Editors	Andrew Y.J. Szeto, Rangaraj M. Rangayyan
Publisher	Publ by IEEE
Pages	1615-1616
Number of pages	2
Edition	pt 3
ISBN (Print)	0780313771
Publication status	Published - 1993
Event	Proceedings of the 15th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Part 3 (of 3) - San Diego, CA, USA Duration: 1993 Oct 28 → 1993 Oct 31

Publication series

Name	Proceedings of the Annual Conference on Engineering in Medicine and Biology
Number	pt 3
Volume	15
ISSN (Print)	0589-1019

Other

Other	Proceedings of the 15th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Part 3 (of 3)
City	San Diego, CA, USA
Period	93/10/28 → 93/10/31

ASJC Scopus subject areas

Signal Processing
Biomedical Engineering
Computer Vision and Pattern Recognition
Health Informatics

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Cite this

Itoh, H., Makimura, N., Arai, T., Tsubamoto, H., Furuya, K., Kikuchi, M., & Nagata, I. (1993). Influence of light irradiation on the development of mouse eggs in culture. In A. Y. J. Szeto, & R. M. Rangayyan (Eds.), Proceedings of the Annual Conference on Engineering in Medicine and Biology (pt 3 ed., pp. 1615-1616). (Proceedings of the Annual Conference on Engineering in Medicine and Biology; Vol. 15, No. pt 3). Publ by IEEE.

Influence of light irradiation on the development of mouse eggs in culture. / Itoh, Harum; Makimura, Noriko; Arai, Tsunenori; Tsubamoto, Hiroshi; Furuya, Kennichi; Kikuchi, Makoto; Nagata, Ichiro.

Proceedings of the Annual Conference on Engineering in Medicine and Biology. ed. / Andrew Y.J. Szeto; Rangaraj M. Rangayyan. pt 3. ed. Publ by IEEE, 1993. p. 1615-1616 (Proceedings of the Annual Conference on Engineering in Medicine and Biology; Vol. 15, No. pt 3).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Itoh, H, Makimura, N, Arai, T, Tsubamoto, H, Furuya, K, Kikuchi, M & Nagata, I 1993, Influence of light irradiation on the development of mouse eggs in culture. in AYJ Szeto & RM Rangayyan (eds), Proceedings of the Annual Conference on Engineering in Medicine and Biology. pt 3 edn, Proceedings of the Annual Conference on Engineering in Medicine and Biology, no. pt 3, vol. 15, Publ by IEEE, pp. 1615-1616, Proceedings of the 15th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Part 3 (of 3), San Diego, CA, USA, 93/10/28.

Itoh H, Makimura N, Arai T, Tsubamoto H, Furuya K, Kikuchi M et al. Influence of light irradiation on the development of mouse eggs in culture. In Szeto AYJ, Rangayyan RM, editors, Proceedings of the Annual Conference on Engineering in Medicine and Biology. pt 3 ed. Publ by IEEE. 1993. p. 1615-1616. (Proceedings of the Annual Conference on Engineering in Medicine and Biology; pt 3).

Itoh, Harum ; Makimura, Noriko ; Arai, Tsunenori ; Tsubamoto, Hiroshi ; Furuya, Kennichi ; Kikuchi, Makoto ; Nagata, Ichiro. / Influence of light irradiation on the development of mouse eggs in culture. Proceedings of the Annual Conference on Engineering in Medicine and Biology. editor / Andrew Y.J. Szeto ; Rangaraj M. Rangayyan. pt 3. ed. Publ by IEEE, 1993. pp. 1615-1616 (Proceedings of the Annual Conference on Engineering in Medicine and Biology; pt 3).

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abstract = "We investigated the influence of light environment on the development of mouse egg in vitro. The 1-cell mouse eggs were irradiated by various laser lights of which wavelengths were 632.8nm, 543nm, and 442nm in vitro. Actual light environment by means of the microscope light source in IVF-ET (in vitro) fertilization-embryo transfer) was measured to set the light dose in this experiment. Mouse eggs were cultured to observe cellular development stage. In case of 632.8nm radiation of 500KJ/m2 in dose (20 times larger than actual light environment), 97% of eggs stopped their division. In the case of 543nm and 442nm radiations no influences were observed in egg division.These results suggested that light environment might affect on the development of mouse egg in vitro.",

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