TY - GEN
T1 - Influence of light irradiation on the development of mouse eggs in culture
AU - Itoh, Harum
AU - Makimura, Noriko
AU - Arai, Tsunenori
AU - Tsubamoto, Hiroshi
AU - Furuya, Kennichi
AU - Kikuchi, Makoto
AU - Nagata, Ichiro
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1993
Y1 - 1993
N2 - We investigated the influence of light environment on the development of mouse egg in vitro. The 1-cell mouse eggs were irradiated by various laser lights of which wavelengths were 632.8nm, 543nm, and 442nm in vitro. Actual light environment by means of the microscope light source in IVF-ET (in vitro) fertilization-embryo transfer) was measured to set the light dose in this experiment. Mouse eggs were cultured to observe cellular development stage. In case of 632.8nm radiation of 500KJ/m2 in dose (20 times larger than actual light environment), 97% of eggs stopped their division. In the case of 543nm and 442nm radiations no influences were observed in egg division.These results suggested that light environment might affect on the development of mouse egg in vitro.
AB - We investigated the influence of light environment on the development of mouse egg in vitro. The 1-cell mouse eggs were irradiated by various laser lights of which wavelengths were 632.8nm, 543nm, and 442nm in vitro. Actual light environment by means of the microscope light source in IVF-ET (in vitro) fertilization-embryo transfer) was measured to set the light dose in this experiment. Mouse eggs were cultured to observe cellular development stage. In case of 632.8nm radiation of 500KJ/m2 in dose (20 times larger than actual light environment), 97% of eggs stopped their division. In the case of 543nm and 442nm radiations no influences were observed in egg division.These results suggested that light environment might affect on the development of mouse egg in vitro.
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M3 - Conference contribution
AN - SCOPUS:0027887074
SN - 0780313771
T3 - Proceedings of the Annual Conference on Engineering in Medicine and Biology
SP - 1615
EP - 1616
BT - Proceedings of the Annual Conference on Engineering in Medicine and Biology
A2 - Szeto, Andrew Y.J.
A2 - Rangayyan, Rangaraj M.
PB - Publ by IEEE
T2 - Proceedings of the 15th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Part 3 (of 3)
Y2 - 28 October 1993 through 31 October 1993
ER -