Abstract
We investigated the influence of light environment on the development of mouse egg in vitro. The 1-cell mouse eggs were irradiated by various laser lights of which wavelengths were 632.8nm, 543nm, and 442nm in vitro. Actual light environment by means of the microscope light source in IVF-ET (in vitro) fertilization-embryo transfer) was measured to set the light dose in this experiment. Mouse eggs were cultured to observe cellular development stage. In case of 632.8nm radiation of 500KJ/m2 in dose (20 times larger than actual light environment), 97% of eggs stopped their division. In the case of 543nm and 442nm radiations no influences were observed in egg division.These results suggested that light environment might affect on the development of mouse egg in vitro.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the Annual Conference on Engineering in Medicine and Biology |
| Editors | Andrew Y.J. Szeto, Rangaraj M. Rangayyan |
| Publisher | Publ by IEEE |
| Pages | 1615-1616 |
| Number of pages | 2 |
| Volume | 15 |
| Edition | pt 3 |
| ISBN (Print) | 0780313771 |
| Publication status | Published - 1993 |
| Externally published | Yes |
| Event | Proceedings of the 15th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Part 3 (of 3) - San Diego, CA, USA Duration: 1993 Oct 28 → 1993 Oct 31 |
Other
| Other | Proceedings of the 15th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Part 3 (of 3) |
|---|---|
| City | San Diego, CA, USA |
| Period | 93/10/28 → 93/10/31 |
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ASJC Scopus subject areas
- Bioengineering
Cite this
Influence of light irradiation on the development of mouse eggs in culture. / Itoh, Harum; Makimura, Noriko; Arai, Tsunenori; Tsubamoto, Hiroshi; Furuya, Kennichi; Kikuchi, Makoto; Nagata, Ichiro.
Proceedings of the Annual Conference on Engineering in Medicine and Biology. ed. / Andrew Y.J. Szeto; Rangaraj M. Rangayyan. Vol. 15 pt 3. ed. Publ by IEEE, 1993. p. 1615-1616.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Influence of light irradiation on the development of mouse eggs in culture
AU - Itoh, Harum
AU - Makimura, Noriko
AU - Arai, Tsunenori
AU - Tsubamoto, Hiroshi
AU - Furuya, Kennichi
AU - Kikuchi, Makoto
AU - Nagata, Ichiro
PY - 1993
Y1 - 1993
N2 - We investigated the influence of light environment on the development of mouse egg in vitro. The 1-cell mouse eggs were irradiated by various laser lights of which wavelengths were 632.8nm, 543nm, and 442nm in vitro. Actual light environment by means of the microscope light source in IVF-ET (in vitro) fertilization-embryo transfer) was measured to set the light dose in this experiment. Mouse eggs were cultured to observe cellular development stage. In case of 632.8nm radiation of 500KJ/m2 in dose (20 times larger than actual light environment), 97% of eggs stopped their division. In the case of 543nm and 442nm radiations no influences were observed in egg division.These results suggested that light environment might affect on the development of mouse egg in vitro.
AB - We investigated the influence of light environment on the development of mouse egg in vitro. The 1-cell mouse eggs were irradiated by various laser lights of which wavelengths were 632.8nm, 543nm, and 442nm in vitro. Actual light environment by means of the microscope light source in IVF-ET (in vitro) fertilization-embryo transfer) was measured to set the light dose in this experiment. Mouse eggs were cultured to observe cellular development stage. In case of 632.8nm radiation of 500KJ/m2 in dose (20 times larger than actual light environment), 97% of eggs stopped their division. In the case of 543nm and 442nm radiations no influences were observed in egg division.These results suggested that light environment might affect on the development of mouse egg in vitro.
UR - http://www.scopus.com/inward/record.url?scp=0027887074&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027887074&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:0027887074
SN - 0780313771
VL - 15
SP - 1615
EP - 1616
BT - Proceedings of the Annual Conference on Engineering in Medicine and Biology
A2 - Szeto, Andrew Y.J.
A2 - Rangayyan, Rangaraj M.
PB - Publ by IEEE
ER -