Abstract
We investigated the process of memory consolidation following classical conditioning of earthworms. Earthworms were conditioned in paired trials by a weak vibration as a conditioned stimulus (CS), and by light as an unconditioned stimulus (US). The occurrence of a shrinking response upon exposure to the CS increased steadily with the number of paired training trials. When the training procedure was changed by increasing the intertrial interval (ITI), it was found that only those worms trained with a 68 s ITI exhibited long-term memory retention for at least 24 h. The influence of mRNA synthesis inhibition by actinomycin-D or of protein synthesis by anisomycin on memory consolidation was also examined. Induction of the long-term memory was blocked when either of these two compounds was injected into the body cavity of the worm within 25 min of conditioning with the 68 s ITI. These results demonstrate that the long-term memory is dependent upon protein synthesis in response to the upregulation of new transcription messengers.
| Original language | English |
|---|---|
| Pages (from-to) | 151-157 |
| Number of pages | 7 |
| Journal | Neurobiology of Learning and Memory |
| Volume | 83 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2005 Mar |
Fingerprint
Keywords
- Actinomycin-D
- Anisomycin
- Associative learning
- Memory consolidation
- Retention of memory
- Shirinkage response
- Ventaral nerve cord
ASJC Scopus subject areas
- Behavioral Neuroscience
- Cognitive Neuroscience
- Experimental and Cognitive Psychology
Cite this
Influence of mRNA and protein synthesis inhibitors on the long-term memory acquisition of classically conditioned earthworms. / Watanabe, Hikaru; Takaya, Tomohiro; Shimoi, Toshinobu; Ogawa, Hiroto; Kitamura, Yoshiichiro; Oka, Kotaro.
In: Neurobiology of Learning and Memory, Vol. 83, No. 2, 03.2005, p. 151-157.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Influence of mRNA and protein synthesis inhibitors on the long-term memory acquisition of classically conditioned earthworms
AU - Watanabe, Hikaru
AU - Takaya, Tomohiro
AU - Shimoi, Toshinobu
AU - Ogawa, Hiroto
AU - Kitamura, Yoshiichiro
AU - Oka, Kotaro
PY - 2005/3
Y1 - 2005/3
N2 - We investigated the process of memory consolidation following classical conditioning of earthworms. Earthworms were conditioned in paired trials by a weak vibration as a conditioned stimulus (CS), and by light as an unconditioned stimulus (US). The occurrence of a shrinking response upon exposure to the CS increased steadily with the number of paired training trials. When the training procedure was changed by increasing the intertrial interval (ITI), it was found that only those worms trained with a 68 s ITI exhibited long-term memory retention for at least 24 h. The influence of mRNA synthesis inhibition by actinomycin-D or of protein synthesis by anisomycin on memory consolidation was also examined. Induction of the long-term memory was blocked when either of these two compounds was injected into the body cavity of the worm within 25 min of conditioning with the 68 s ITI. These results demonstrate that the long-term memory is dependent upon protein synthesis in response to the upregulation of new transcription messengers.
AB - We investigated the process of memory consolidation following classical conditioning of earthworms. Earthworms were conditioned in paired trials by a weak vibration as a conditioned stimulus (CS), and by light as an unconditioned stimulus (US). The occurrence of a shrinking response upon exposure to the CS increased steadily with the number of paired training trials. When the training procedure was changed by increasing the intertrial interval (ITI), it was found that only those worms trained with a 68 s ITI exhibited long-term memory retention for at least 24 h. The influence of mRNA synthesis inhibition by actinomycin-D or of protein synthesis by anisomycin on memory consolidation was also examined. Induction of the long-term memory was blocked when either of these two compounds was injected into the body cavity of the worm within 25 min of conditioning with the 68 s ITI. These results demonstrate that the long-term memory is dependent upon protein synthesis in response to the upregulation of new transcription messengers.
KW - Actinomycin-D
KW - Anisomycin
KW - Associative learning
KW - Memory consolidation
KW - Retention of memory
KW - Shirinkage response
KW - Ventaral nerve cord
UR - http://www.scopus.com/inward/record.url?scp=13844297836&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=13844297836&partnerID=8YFLogxK
U2 - 10.1016/j.nlm.2004.11.003
DO - 10.1016/j.nlm.2004.11.003
M3 - Article
VL - 83
SP - 151
EP - 157
JO - Neurobiology of Learning and Memory
JF - Neurobiology of Learning and Memory
SN - 1074-7427
IS - 2
ER -