Influence of the structure of support microspheres on leucocyte activation by RGDS-carrying microspheres

Yuji Kasuya; Keiji Fujimoto; Haruma Kawaguchi; Masaki Miyamoto

doi:10.1016/0142-9612(94)90206-2

Influence of the structure of support microspheres on leucocyte activation by RGDS-carrying microspheres

Yuji Kasuya, Keiji Fujimoto, Haruma Kawaguchi, Masaki Miyamoto

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

A cell adhesive peptide, Arg-Gly-Asp-Ser (RGDS), enhances leucocyte response to stimuli when insolubilized or conjugated with proteins. In order to investigate the contribution of the microsphere structure to leucocyte response, RGDS was immobilized onto several polymeric microspheres. The respiratory burst of polymorphonuclear leucocytes (PMNs) contacting microspheres was enhanced by RGDS in cases where it was immobilized on polystyrene and, more effectively, a positively charged one. This was not observed in polyacrylamide-based or negative-charged microsphere systems. These results demonstrate that the property of supports strongly affects the regulation of the PMN response via an adhesive interaction.

Original language	English
Pages (from-to)	570-576
Number of pages	7
Journal	Biomaterials
Volume	15
Issue number	8
DOIs	https://doi.org/10.1016/0142-9612(94)90206-2
Publication status	Published - 1994 Jun

Keywords

Microspheres
cell adhesion
phagocytosis
polymorphonuclear leucocytes

ASJC Scopus subject areas

Bioengineering
Ceramics and Composites
Biophysics
Biomaterials
Mechanics of Materials

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10.1016/0142-9612(94)90206-2

Cite this

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title = "Influence of the structure of support microspheres on leucocyte activation by RGDS-carrying microspheres",

abstract = "A cell adhesive peptide, Arg-Gly-Asp-Ser (RGDS), enhances leucocyte response to stimuli when insolubilized or conjugated with proteins. In order to investigate the contribution of the microsphere structure to leucocyte response, RGDS was immobilized onto several polymeric microspheres. The respiratory burst of polymorphonuclear leucocytes (PMNs) contacting microspheres was enhanced by RGDS in cases where it was immobilized on polystyrene and, more effectively, a positively charged one. This was not observed in polyacrylamide-based or negative-charged microsphere systems. These results demonstrate that the property of supports strongly affects the regulation of the PMN response via an adhesive interaction.",

keywords = "Microspheres, cell adhesion, phagocytosis, polymorphonuclear leucocytes",

author = "Yuji Kasuya and Keiji Fujimoto and Haruma Kawaguchi and Masaki Miyamoto",

note = "Funding Information: This work was supported by a Grant-in-Aid fic Research on Priority Areas from the Education, Science and Culture of Japan. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

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AU - Kasuya, Yuji

AU - Fujimoto, Keiji

AU - Kawaguchi, Haruma

AU - Miyamoto, Masaki

N1 - Funding Information: This work was supported by a Grant-in-Aid fic Research on Priority Areas from the Education, Science and Culture of Japan. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

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N2 - A cell adhesive peptide, Arg-Gly-Asp-Ser (RGDS), enhances leucocyte response to stimuli when insolubilized or conjugated with proteins. In order to investigate the contribution of the microsphere structure to leucocyte response, RGDS was immobilized onto several polymeric microspheres. The respiratory burst of polymorphonuclear leucocytes (PMNs) contacting microspheres was enhanced by RGDS in cases where it was immobilized on polystyrene and, more effectively, a positively charged one. This was not observed in polyacrylamide-based or negative-charged microsphere systems. These results demonstrate that the property of supports strongly affects the regulation of the PMN response via an adhesive interaction.

AB - A cell adhesive peptide, Arg-Gly-Asp-Ser (RGDS), enhances leucocyte response to stimuli when insolubilized or conjugated with proteins. In order to investigate the contribution of the microsphere structure to leucocyte response, RGDS was immobilized onto several polymeric microspheres. The respiratory burst of polymorphonuclear leucocytes (PMNs) contacting microspheres was enhanced by RGDS in cases where it was immobilized on polystyrene and, more effectively, a positively charged one. This was not observed in polyacrylamide-based or negative-charged microsphere systems. These results demonstrate that the property of supports strongly affects the regulation of the PMN response via an adhesive interaction.

KW - Microspheres

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KW - phagocytosis

KW - polymorphonuclear leucocytes

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Influence of the structure of support microspheres on leucocyte activation by RGDS-carrying microspheres

Abstract

Keywords

ASJC Scopus subject areas

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