Influence of the structure of support microspheres on leucocyte activation by RGDS-carrying microspheres

Yuji Kasuya, Keiji Fujimoto, Haruma Kawaguchi, Masaki Miyamoto

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A cell adhesive peptide, Arg-Gly-Asp-Ser (RGDS), enhances leucocyte response to stimuli when insolubilized or conjugated with proteins. In order to investigate the contribution of the microsphere structure to leucocyte response, RGDS was immobilized onto several polymeric microspheres. The respiratory burst of polymorphonuclear leucocytes (PMNs) contacting microspheres was enhanced by RGDS in cases where it was immobilized on polystyrene and, more effectively, a positively charged one. This was not observed in polyacrylamide-based or negative-charged microsphere systems. These results demonstrate that the property of supports strongly affects the regulation of the PMN response via an adhesive interaction.

Original languageEnglish
Pages (from-to)570-576
Number of pages7
JournalBiomaterials
Volume15
Issue number8
DOIs
Publication statusPublished - 1994 Jun

Keywords

  • Microspheres
  • cell adhesion
  • phagocytosis
  • polymorphonuclear leucocytes

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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