Inhalation of hydrogen gas suppresses hepatic injury caused by ischemia/reperfusion through reducing oxidative stress

Kei ichi Fukuda, Sadamitsu Asoh, Masahiro Ishikawa, Yasuhiro Yamamoto, Ikuroh Ohsawa, Shigeo Ohta

Research output: Contribution to journalArticle

294 Citations (Scopus)

Abstract

We have recently showed that molecular hydrogen has great potential for selectively reducing cytotoxic reactive oxygen species, such as hydroxyl radicals, and that inhalation of hydrogen gas decreases cerebral infarction volume by reducing oxidative stress [I. Ohsawa, M. Ishikawa, K. Takahashi, M. Watanabe, K. Nishimaki, K. Yamagata, K.-I. Katsura, Y. Katayama, S. Asoh, S. Ohta, Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals, Nat. Med., 13 (2007) 688-694]. Here we show that the inhalation of hydrogen gas is applicable for hepatic injury caused by ischemia/reperfusion, using mice. The portal triad to the left lobe and the left middle lobe of the liver were completely occluded for 90 min, followed by reperfusion for 180 min. Inhalation of hydrogen gas (1-4%) during the last 190 min suppressed hepatic cell death, and reduced levels of serum alanine aminotransferase and hepatic malondialdehyde. In contrast, helium gas showed no protective effect, suggesting that the protective effect by hydrogen gas is specific. Thus, we propose that inhalation of hydrogen gas is a widely applicable method to reduce oxidative stress.

Original languageEnglish
Pages (from-to)670-674
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume361
Issue number3
DOIs
Publication statusPublished - 2007 Sep 28
Externally publishedYes

Keywords

  • ALT
  • Anti-oxidant
  • Hydrogen gas
  • Hydroxyl radical
  • Ischemia
  • Liver
  • MDA
  • Oxidative stress
  • Reperfusion

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Inhalation of hydrogen gas suppresses hepatic injury caused by ischemia/reperfusion through reducing oxidative stress'. Together they form a unique fingerprint.

  • Cite this