TY - JOUR
T1 - Inheritance of a Nuclear PIWI from Pluripotent Stem Cells by Somatic Descendants Ensures Differentiation by Silencing Transposons in Planarian
AU - Shibata, Norito
AU - Kashima, Makoto
AU - Ishiko, Taisuke
AU - Nishimura, Osamu
AU - Rouhana, Labib
AU - Misaki, Kazuyo
AU - Yonemura, Shigenobu
AU - Saito, Kuniaki
AU - Siomi, Haruhiko
AU - Siomi, Mikiko C.
AU - Agata, Kiyokazu
N1 - Funding Information:
We are grateful to Elizabeth Nakajima, Hayoung Lee, Nobuyoshi Kumagai, Yuki Sato, and Takeshi Inoue for critical reading of the manuscript and to Prof. Yoshio Koyanagi of the Institute for Virus Research, Kyoto University, and Prof. Koichi Sano of Osaka Medical College for commenting on the transmission electron microscopy analysis. We also thank all of our laboratory members for their help and encouragement. This work was supported by a Grant-in-Aid for Scientific Research on Innovative Areas to K.A. ( 22124001 ), a Grant-in-Aid for Creative Scientific Research to K.A. (17GS0318), Global COE Program A06 of Kyoto University , the Naito Foundation , a Sasakawa Scientific Research Grant, the Future Development Funding Program of Kyoto University Research Coordination Alliance , a Grant-in-Aid for Scientific Research on Innovative Areas “RNA regulation” ( 20112006 ) to N.S., a JSPS Research Fellowship to M.K., and a Minority Postdoctoral Research Fellowship from the NSF (No. 0804021) to L.R.
Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/5/9
Y1 - 2016/5/9
N2 - Differentiation of pluripotent stem cells (PSCs) requires transposon silencing throughout the process. PIWIs, best known as key factors in germline transposon silencing, are also known to act in somatic differentiation of planarian PSCs (neoblasts). However, how PIWIs control the latter process remains elusive. Here, using Dugesia japonica, we show that a nuclear PIWI, DjPiwiB, was bound to PIWI-interacting RNAs (generally key mediators of PIWI-dependent transposon silencing), and was detected in not only neoblasts but also their descendant somatic cells, which do not express piwi. In contrast, cytoplasmic DjPiwiA and DjPiwiC were detected only in neoblasts, in accord with their transcription there. DjPiwiB was indispensable for regeneration, but dispensable for transposon silencing in neoblasts. However, transposons were derepressed at the onset of differentiation in DjPiwiB-knockdown planarians. Thus, DjPiwiB appears to be inherited by descendant somatic cells of neoblasts to ensure transposon silencing in those cells, which are unable to produce PIWI proteins.
AB - Differentiation of pluripotent stem cells (PSCs) requires transposon silencing throughout the process. PIWIs, best known as key factors in germline transposon silencing, are also known to act in somatic differentiation of planarian PSCs (neoblasts). However, how PIWIs control the latter process remains elusive. Here, using Dugesia japonica, we show that a nuclear PIWI, DjPiwiB, was bound to PIWI-interacting RNAs (generally key mediators of PIWI-dependent transposon silencing), and was detected in not only neoblasts but also their descendant somatic cells, which do not express piwi. In contrast, cytoplasmic DjPiwiA and DjPiwiC were detected only in neoblasts, in accord with their transcription there. DjPiwiB was indispensable for regeneration, but dispensable for transposon silencing in neoblasts. However, transposons were derepressed at the onset of differentiation in DjPiwiB-knockdown planarians. Thus, DjPiwiB appears to be inherited by descendant somatic cells of neoblasts to ensure transposon silencing in those cells, which are unable to produce PIWI proteins.
UR - http://www.scopus.com/inward/record.url?scp=84975066647&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84975066647&partnerID=8YFLogxK
U2 - 10.1016/j.devcel.2016.04.009
DO - 10.1016/j.devcel.2016.04.009
M3 - Article
C2 - 27165555
AN - SCOPUS:84975066647
SN - 1534-5807
VL - 37
SP - 226
EP - 237
JO - Developmental Cell
JF - Developmental Cell
IS - 3
ER -