Inhibition by endothelial cells of platelet aggregating activity of thrombin - role of thrombomodulin

Mitsuru Murata, Y. Ikeda, Y. Araki, H. Murakami, K. Sato, M. Yamamoto, K. Watanabe, Y. Ando, T. Igawa, I. Maruyama

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cultured human umbilical vein endothelial cells inhibited the platelet aggregating activity of thrombin in the absence of plasma. Using a new method in which thrombin-induced platelet aggregation was measured in the presence of endothelial cells, we showed that aspirin-treated endothelial cells inhibited platelet aggregating activity of thrombin in an incubation time- and cell number-dependent manner. This inhibitory effect of endothelial cells was partially blocked by the pretreatment of endothelial cells with monoclonal anti-thrombomodulin IgG(anti-TMIgG). It is suggested that (1) endothelial cells play a role in the clearance of thrombin by binding and inactivating this enzyme and that (2) thrombomodulin on endothelial cells, apart from its role for protein C activation, may be involved in this endothelial function.

Original languageEnglish
Pages (from-to)647-656
Number of pages10
JournalThrombosis Research
Volume50
Issue number5
DOIs
Publication statusPublished - 1988 Jun 1

Fingerprint

Thrombomodulin
Thrombin
Blood Platelets
Endothelial Cells
Human Umbilical Vein Endothelial Cells
Protein C
Platelet Aggregation
Aspirin
Cell Count
Enzymes

Keywords

  • endothelial cells
  • platelet aggregation
  • thrombin
  • thrombomodulin

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

Inhibition by endothelial cells of platelet aggregating activity of thrombin - role of thrombomodulin. / Murata, Mitsuru; Ikeda, Y.; Araki, Y.; Murakami, H.; Sato, K.; Yamamoto, M.; Watanabe, K.; Ando, Y.; Igawa, T.; Maruyama, I.

In: Thrombosis Research, Vol. 50, No. 5, 01.06.1988, p. 647-656.

Research output: Contribution to journalArticle

Murata, M, Ikeda, Y, Araki, Y, Murakami, H, Sato, K, Yamamoto, M, Watanabe, K, Ando, Y, Igawa, T & Maruyama, I 1988, 'Inhibition by endothelial cells of platelet aggregating activity of thrombin - role of thrombomodulin', Thrombosis Research, vol. 50, no. 5, pp. 647-656. https://doi.org/10.1016/0049-3848(88)90323-4
Murata, Mitsuru ; Ikeda, Y. ; Araki, Y. ; Murakami, H. ; Sato, K. ; Yamamoto, M. ; Watanabe, K. ; Ando, Y. ; Igawa, T. ; Maruyama, I. / Inhibition by endothelial cells of platelet aggregating activity of thrombin - role of thrombomodulin. In: Thrombosis Research. 1988 ; Vol. 50, No. 5. pp. 647-656.
@article{6901ae242d8b4dc3b0c43d5d8cd28ffd,
title = "Inhibition by endothelial cells of platelet aggregating activity of thrombin - role of thrombomodulin",
abstract = "Cultured human umbilical vein endothelial cells inhibited the platelet aggregating activity of thrombin in the absence of plasma. Using a new method in which thrombin-induced platelet aggregation was measured in the presence of endothelial cells, we showed that aspirin-treated endothelial cells inhibited platelet aggregating activity of thrombin in an incubation time- and cell number-dependent manner. This inhibitory effect of endothelial cells was partially blocked by the pretreatment of endothelial cells with monoclonal anti-thrombomodulin IgG(anti-TMIgG). It is suggested that (1) endothelial cells play a role in the clearance of thrombin by binding and inactivating this enzyme and that (2) thrombomodulin on endothelial cells, apart from its role for protein C activation, may be involved in this endothelial function.",
keywords = "endothelial cells, platelet aggregation, thrombin, thrombomodulin",
author = "Mitsuru Murata and Y. Ikeda and Y. Araki and H. Murakami and K. Sato and M. Yamamoto and K. Watanabe and Y. Ando and T. Igawa and I. Maruyama",
year = "1988",
month = "6",
day = "1",
doi = "10.1016/0049-3848(88)90323-4",
language = "English",
volume = "50",
pages = "647--656",
journal = "Thrombosis Research",
issn = "0049-3848",
publisher = "Elsevier Limited",
number = "5",

}

TY - JOUR

T1 - Inhibition by endothelial cells of platelet aggregating activity of thrombin - role of thrombomodulin

AU - Murata, Mitsuru

AU - Ikeda, Y.

AU - Araki, Y.

AU - Murakami, H.

AU - Sato, K.

AU - Yamamoto, M.

AU - Watanabe, K.

AU - Ando, Y.

AU - Igawa, T.

AU - Maruyama, I.

PY - 1988/6/1

Y1 - 1988/6/1

N2 - Cultured human umbilical vein endothelial cells inhibited the platelet aggregating activity of thrombin in the absence of plasma. Using a new method in which thrombin-induced platelet aggregation was measured in the presence of endothelial cells, we showed that aspirin-treated endothelial cells inhibited platelet aggregating activity of thrombin in an incubation time- and cell number-dependent manner. This inhibitory effect of endothelial cells was partially blocked by the pretreatment of endothelial cells with monoclonal anti-thrombomodulin IgG(anti-TMIgG). It is suggested that (1) endothelial cells play a role in the clearance of thrombin by binding and inactivating this enzyme and that (2) thrombomodulin on endothelial cells, apart from its role for protein C activation, may be involved in this endothelial function.

AB - Cultured human umbilical vein endothelial cells inhibited the platelet aggregating activity of thrombin in the absence of plasma. Using a new method in which thrombin-induced platelet aggregation was measured in the presence of endothelial cells, we showed that aspirin-treated endothelial cells inhibited platelet aggregating activity of thrombin in an incubation time- and cell number-dependent manner. This inhibitory effect of endothelial cells was partially blocked by the pretreatment of endothelial cells with monoclonal anti-thrombomodulin IgG(anti-TMIgG). It is suggested that (1) endothelial cells play a role in the clearance of thrombin by binding and inactivating this enzyme and that (2) thrombomodulin on endothelial cells, apart from its role for protein C activation, may be involved in this endothelial function.

KW - endothelial cells

KW - platelet aggregation

KW - thrombin

KW - thrombomodulin

UR - http://www.scopus.com/inward/record.url?scp=0023952141&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023952141&partnerID=8YFLogxK

U2 - 10.1016/0049-3848(88)90323-4

DO - 10.1016/0049-3848(88)90323-4

M3 - Article

C2 - 2842885

AN - SCOPUS:0023952141

VL - 50

SP - 647

EP - 656

JO - Thrombosis Research

JF - Thrombosis Research

SN - 0049-3848

IS - 5

ER -