Inhibition by endothelial cells of platelet aggregating activity of thrombin - role of thrombomodulin

M. Murata, Y. Ikeda, Y. Araki, H. Murakami, K. Sato, M. Yamamoto, K. Watanabe, Y. Ando, T. Igawa, I. Maruyama

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Abstract

Cultured human umbilical vein endothelial cells inhibited the platelet aggregating activity of thrombin in the absence of plasma. Using a new method in which thrombin-induced platelet aggregation was measured in the presence of endothelial cells, we showed that aspirin-treated endothelial cells inhibited platelet aggregating activity of thrombin in an incubation time- and cell number-dependent manner. This inhibitory effect of endothelial cells was partially blocked by the pretreatment of endothelial cells with monoclonal anti-thrombomodulin IgG(anti-TMIgG). It is suggested that (1) endothelial cells play a role in the clearance of thrombin by binding and inactivating this enzyme and that (2) thrombomodulin on endothelial cells, apart from its role for protein C activation, may be involved in this endothelial function.

Original languageEnglish
Pages (from-to)647-656
Number of pages10
JournalThrombosis Research
Volume50
Issue number5
DOIs
Publication statusPublished - 1988 Jun 1

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Keywords

  • endothelial cells
  • platelet aggregation
  • thrombin
  • thrombomodulin

ASJC Scopus subject areas

  • Hematology

Cite this

Murata, M., Ikeda, Y., Araki, Y., Murakami, H., Sato, K., Yamamoto, M., Watanabe, K., Ando, Y., Igawa, T., & Maruyama, I. (1988). Inhibition by endothelial cells of platelet aggregating activity of thrombin - role of thrombomodulin. Thrombosis Research, 50(5), 647-656. https://doi.org/10.1016/0049-3848(88)90323-4