Abstract
Cultured human umbilical vein endothelial cells inhibited the platelet aggregating activity of thrombin in the absence of plasma. Using a new method in which thrombin-induced platelet aggregation was measured in the presence of endothelial cells, we showed that aspirin-treated endothelial cells inhibited platelet aggregating activity of thrombin in an incubation time- and cell number-dependent manner. This inhibitory effect of endothelial cells was partially blocked by the pretreatment of endothelial cells with monoclonal anti-thrombomodulin IgG(anti-TMIgG). It is suggested that (1) endothelial cells play a role in the clearance of thrombin by binding and inactivating this enzyme and that (2) thrombomodulin on endothelial cells, apart from its role for protein C activation, may be involved in this endothelial function.
| Original language | English |
|---|---|
| Pages (from-to) | 647-656 |
| Number of pages | 10 |
| Journal | Thrombosis Research |
| Volume | 50 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1988 Jun 1 |
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Keywords
- endothelial cells
- platelet aggregation
- thrombin
- thrombomodulin
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
- Hematology
Cite this
Inhibition by endothelial cells of platelet aggregating activity of thrombin - role of thrombomodulin. / Murata, Mitsuru; Ikeda, Y.; Araki, Y.; Murakami, H.; Sato, K.; Yamamoto, M.; Watanabe, K.; Ando, Y.; Igawa, T.; Maruyama, I.
In: Thrombosis Research, Vol. 50, No. 5, 01.06.1988, p. 647-656.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition by endothelial cells of platelet aggregating activity of thrombin - role of thrombomodulin
AU - Murata, Mitsuru
AU - Ikeda, Y.
AU - Araki, Y.
AU - Murakami, H.
AU - Sato, K.
AU - Yamamoto, M.
AU - Watanabe, K.
AU - Ando, Y.
AU - Igawa, T.
AU - Maruyama, I.
PY - 1988/6/1
Y1 - 1988/6/1
N2 - Cultured human umbilical vein endothelial cells inhibited the platelet aggregating activity of thrombin in the absence of plasma. Using a new method in which thrombin-induced platelet aggregation was measured in the presence of endothelial cells, we showed that aspirin-treated endothelial cells inhibited platelet aggregating activity of thrombin in an incubation time- and cell number-dependent manner. This inhibitory effect of endothelial cells was partially blocked by the pretreatment of endothelial cells with monoclonal anti-thrombomodulin IgG(anti-TMIgG). It is suggested that (1) endothelial cells play a role in the clearance of thrombin by binding and inactivating this enzyme and that (2) thrombomodulin on endothelial cells, apart from its role for protein C activation, may be involved in this endothelial function.
AB - Cultured human umbilical vein endothelial cells inhibited the platelet aggregating activity of thrombin in the absence of plasma. Using a new method in which thrombin-induced platelet aggregation was measured in the presence of endothelial cells, we showed that aspirin-treated endothelial cells inhibited platelet aggregating activity of thrombin in an incubation time- and cell number-dependent manner. This inhibitory effect of endothelial cells was partially blocked by the pretreatment of endothelial cells with monoclonal anti-thrombomodulin IgG(anti-TMIgG). It is suggested that (1) endothelial cells play a role in the clearance of thrombin by binding and inactivating this enzyme and that (2) thrombomodulin on endothelial cells, apart from its role for protein C activation, may be involved in this endothelial function.
KW - endothelial cells
KW - platelet aggregation
KW - thrombin
KW - thrombomodulin
UR - http://www.scopus.com/inward/record.url?scp=0023952141&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023952141&partnerID=8YFLogxK
U2 - 10.1016/0049-3848(88)90323-4
DO - 10.1016/0049-3848(88)90323-4
M3 - Article
C2 - 2842885
AN - SCOPUS:0023952141
VL - 50
SP - 647
EP - 656
JO - Thrombosis Research
JF - Thrombosis Research
SN - 0049-3848
IS - 5
ER -