Inhibition of 12-O-tetradecanoylphorbol-13-acetate-induced incease in vascular permeability in mouse skin by lipoxygenase inhibitors

T. Nakadate, S. Yamamoto, Eriko Yokota, R. Kato

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Abstract

The painting of mouse dorsal skin with 12-O-tetradecanoylphorbol-13-acetate (TPA) (0.2-2.5 nmol/mouse) induced a dose-related increase in vascular permeability, which was determined by pontamine sky blue exudation into the skin 5 hr after the TPA treatment. Marked infiltration of neutrophils in the dermal interstitium was also observed 5 hr after TPA treatment. Treatment of mice with nordihydroguaiaretic acid (NDGA) (10 μmol/mouse), 2,3,5-trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1,4-benzoquinone (AA861) (10 μmol/mouse) and quercetin (3 μmol/mouse) significantly inhibited the TPA-induced dye exudation. However, indomethacin (250-1000 nmol/mouse) tended to inhibit the TPA-induced dye exudation, but the inhibition was not statistically significant. Treatment with AA861 (10 μmol/mouse) also caused a marked inhibition of TPA-induced neutrophil infiltration. Quercetin, NDGA and AA861 inhibited epidermal lipoxygenase activity, but indomethacin failed to inhibit it. On the other hand, indomethacin inhibited epidermal cyclooxygenase, but quercetin, NDGA and AA861 failed to inhibit it. The present study suggests involvement of a lipoxygenase product(s) in the mechanism of the TPA-induced increase in vascular permeability in the dorsal skin of mice.

Original languageEnglish
Pages (from-to)161-168
Number of pages8
JournalJapanese Journal of Pharmacology
Volume38
Issue number2
Publication statusPublished - 1985

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Lipoxygenase Inhibitors
Capillary Permeability
Tetradecanoylphorbol Acetate
Skin
Masoprocol
Quercetin
Indomethacin
Lipoxygenase
Neutrophil Infiltration
Coloring Agents
Paintings
Prostaglandin-Endoperoxide Synthases
2,3,5-trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1,4-benzoquinone

ASJC Scopus subject areas

  • Pharmacology

Cite this

Inhibition of 12-O-tetradecanoylphorbol-13-acetate-induced incease in vascular permeability in mouse skin by lipoxygenase inhibitors. / Nakadate, T.; Yamamoto, S.; Yokota, Eriko; Kato, R.

In: Japanese Journal of Pharmacology, Vol. 38, No. 2, 1985, p. 161-168.

Research output: Contribution to journalArticle

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