TY - JOUR
T1 - Inhibition of 12-O-Tetradecanoylphorbol-13-Acetate-Induced Increase in Vascular Permeability in Mouse Skin by Lipoxygenase Inhibitors
AU - Nakadate, Teruo
AU - Yamamoto, Satoshi
AU - Aizu, Eriko
AU - Kato, Ryuichi
PY - 1985
Y1 - 1985
N2 - The painting of mouse dorsal skin with 1 2-0-tetradecanoylphorbol-l3-acetate (TPA) (0.2-2.5 nrTiol/mouse) induced a dose-related increase in vascular permeability, which was determined by pontamine sky blue exudation mto the skin 5 hr after the TPA treatment. Marked infiltration of neutrophils in the dermal interstitium was also observed 5 hr after TPA treatment. Treatment of mice with nordihydroguaiaretic acid (NDGA) (10 µmol/mouse), 2,3,5-trimethyl-6-(12-hydroxy-5,1 0-dodecadiynyl)-1,4-benzoquinone (AA861) (10 µmol/mouse) and quercetm (3µmol/mouse) significantly inhibited the TPA-mduced dye exudation. However, indomethacin (250-1000µmol/mouse) tended to inhibit the TPA-mduced dye exudation, but the inhibition was not statistically significant. Treatment with AA861 (10µmol/mouse) also caused a marked inhibition of TPA-mduced neutrophil infiltration. Quercetm, NDGA and AA861 inhibited epidermal lipoxygenase activity, but indomethacin failed to inhibit it. On the other hand, indomethacin inhibited epidermal cyclooxygenase, but quercetm, NDGA and AA861 failed to inhibit it. The present study suggests involvement of a lipoxygenase product(s) in the mechanism of the TPA-induced increase in vascular permeability in the dorsal skin of mice.
AB - The painting of mouse dorsal skin with 1 2-0-tetradecanoylphorbol-l3-acetate (TPA) (0.2-2.5 nrTiol/mouse) induced a dose-related increase in vascular permeability, which was determined by pontamine sky blue exudation mto the skin 5 hr after the TPA treatment. Marked infiltration of neutrophils in the dermal interstitium was also observed 5 hr after TPA treatment. Treatment of mice with nordihydroguaiaretic acid (NDGA) (10 µmol/mouse), 2,3,5-trimethyl-6-(12-hydroxy-5,1 0-dodecadiynyl)-1,4-benzoquinone (AA861) (10 µmol/mouse) and quercetm (3µmol/mouse) significantly inhibited the TPA-mduced dye exudation. However, indomethacin (250-1000µmol/mouse) tended to inhibit the TPA-mduced dye exudation, but the inhibition was not statistically significant. Treatment with AA861 (10µmol/mouse) also caused a marked inhibition of TPA-mduced neutrophil infiltration. Quercetm, NDGA and AA861 inhibited epidermal lipoxygenase activity, but indomethacin failed to inhibit it. On the other hand, indomethacin inhibited epidermal cyclooxygenase, but quercetm, NDGA and AA861 failed to inhibit it. The present study suggests involvement of a lipoxygenase product(s) in the mechanism of the TPA-induced increase in vascular permeability in the dorsal skin of mice.
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U2 - 10.1254/jjp.38.161
DO - 10.1254/jjp.38.161
M3 - Article
C2 - 3928951
AN - SCOPUS:0021958696
SN - 0021-5198
VL - 38
SP - 161
EP - 168
JO - Japanese Journal of Pharmacology
JF - Japanese Journal of Pharmacology
IS - 2
ER -