Inhibition of attachment and chemotactic invasion of uterine endometrial cancer cells by a new vinca alkaloid, conophylline

Takuya Irie, Kaneyuki Kubushiro, Kanya Suzuki, Katsumi Tsukazaki, Kazuo Umezawa, Shiro Nozawa

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The effect of conophylline, a new vinca alkaloid that inhibits ras expression, on tumor cell adhesion and infiltration was evaluated using a human endometrial cancer cell line. When SNG-II, a highly differentiated human endometrial cancer cell line, was exposed to conophylline, the cells developed filamentous processes at concentrations which did not affect cell proliferation (0.03-0.3 μg/ml). After exposure to conophylline (0.3 μg/ml), cells adherent to matrigel- and type IV collagen-coated wells respecively decreased to 26.9% and 33.3% of the number in the untreated control culture (p < 0.01). In an in vitro invasion assay using a Boyden chamber, infiltration of cells exposed to conophylline decreased to 19.4% (0.3 μg/ml) (p < 0.01) of the control. In a wound assay, conophylline inhibited the movement of cells at 24 hr after wounding. Flow cytometric analysis revealed that expression of integrin β1 was not altered by conophylline, but E-cadherin and CD44 were decreased. The expression of E-cadherin and CD44 could be changed by conophlline.

Original languageEnglish
Pages (from-to)3061-3066
Number of pages6
JournalAnticancer Research
Volume19
Issue number4 B
Publication statusPublished - 1999

Fingerprint

Vinca Alkaloids
Uterine Neoplasms
Endometrial Neoplasms
Cadherins
Cell Line
Collagen Type IV
Cell Adhesion
Integrins
Cell Movement
conophylline
Cell Proliferation
Wounds and Injuries
Neoplasms

Keywords

  • Boyden chamber
  • Conophylline
  • K-ras
  • Uterine endometrial cancer

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Irie, T., Kubushiro, K., Suzuki, K., Tsukazaki, K., Umezawa, K., & Nozawa, S. (1999). Inhibition of attachment and chemotactic invasion of uterine endometrial cancer cells by a new vinca alkaloid, conophylline. Anticancer Research, 19(4 B), 3061-3066.

Inhibition of attachment and chemotactic invasion of uterine endometrial cancer cells by a new vinca alkaloid, conophylline. / Irie, Takuya; Kubushiro, Kaneyuki; Suzuki, Kanya; Tsukazaki, Katsumi; Umezawa, Kazuo; Nozawa, Shiro.

In: Anticancer Research, Vol. 19, No. 4 B, 1999, p. 3061-3066.

Research output: Contribution to journalArticle

Irie, T, Kubushiro, K, Suzuki, K, Tsukazaki, K, Umezawa, K & Nozawa, S 1999, 'Inhibition of attachment and chemotactic invasion of uterine endometrial cancer cells by a new vinca alkaloid, conophylline', Anticancer Research, vol. 19, no. 4 B, pp. 3061-3066.
Irie, Takuya ; Kubushiro, Kaneyuki ; Suzuki, Kanya ; Tsukazaki, Katsumi ; Umezawa, Kazuo ; Nozawa, Shiro. / Inhibition of attachment and chemotactic invasion of uterine endometrial cancer cells by a new vinca alkaloid, conophylline. In: Anticancer Research. 1999 ; Vol. 19, No. 4 B. pp. 3061-3066.
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