Inhibition of CDP-DG

iNositol transferase by inostamycin

Masaya Imoto, Yoshiko Taniguchi, Kazuo Umezawa

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Inostamycin, a novel microbial secondary metabolite, inhibited [3H]inositol and 32P1 incorporation into phosphatidylinositol (PtdIns) induced by epidermal growth factor (EGF) in cultured A431 cells, the IC50 being 0.5 μg/ml, without inhibiting macromolecular synthesis. The drug inhibited cellular inositol phosphate formation only when it was added at the same time as labeled inositol. It was found to inhibit in vitro CDP-DG:inositol transferase activity of the A431 cell membrane, the IC50 being about 0.02 μg/ml. It did not inhibit tyrosine kinase, PtdIns phospholipase C, or PtdIns kinase. Therefore, inhibition of PtdIns turnover by inostamycin must be due to the inhibition of CDP-DG:inositol transferase. Thus, inostamycin is a novel inhibitor of CDP-DG:inositol transferase.

Original languageEnglish
Pages (from-to)299-302
Number of pages4
JournalJournal of Biochemistry
Volume112
Issue number2
Publication statusPublished - 1992 Aug

Fingerprint

CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase
Cell membranes
Metabolites
Phosphatidylinositols
Phosphates
Cultured Cells
Growth Factors
Inositol
Phosphate
Inhibitory Concentration 50
Inhibitor
Drugs
Membrane
Synthesis
Inositol Phosphates
Cell
Type C Phospholipases
Epidermal Growth Factor
Protein-Tyrosine Kinases
Phosphotransferases

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Imoto, M., Taniguchi, Y., & Umezawa, K. (1992). Inhibition of CDP-DG: iNositol transferase by inostamycin. Journal of Biochemistry, 112(2), 299-302.

Inhibition of CDP-DG : iNositol transferase by inostamycin. / Imoto, Masaya; Taniguchi, Yoshiko; Umezawa, Kazuo.

In: Journal of Biochemistry, Vol. 112, No. 2, 08.1992, p. 299-302.

Research output: Contribution to journalArticle

Imoto, M, Taniguchi, Y & Umezawa, K 1992, 'Inhibition of CDP-DG: iNositol transferase by inostamycin', Journal of Biochemistry, vol. 112, no. 2, pp. 299-302.
Imoto, Masaya ; Taniguchi, Yoshiko ; Umezawa, Kazuo. / Inhibition of CDP-DG : iNositol transferase by inostamycin. In: Journal of Biochemistry. 1992 ; Vol. 112, No. 2. pp. 299-302.
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