Inhibition of cell growth by bafilomycin A1, a selective inhibitor of vacuolar H+-ATPase

Shoji Ohkuma; Sakae Shimizu; Masahiko Noto; Yoshimichi Sai; Kuninori Kinoshita; Hiro Omi Tamura

doi:10.1007/BF02631364

Inhibition of cell growth by bafilomycin A₁, a selective inhibitor of vacuolar H⁺-ATPase

Shoji Ohkuma, Sakae Shimizu, Masahiko Noto, Yoshimichi Sai, Kuninori Kinoshita, Hiro Omi Tamura

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

Bafilomycin A₁, a potent selective inhibitor of vacuolar H⁺-ATPase, inhibited the growth of a variety of cultured cells dose-dependently, including golden hamster embryo and NIH-3T3 fibroblasts, whether or not they were transformed, and PC12 and HeLa cells. The concentration of bafilomycin A₁ for 50% inhibition of cell growth ranged from 10 to 50 n M. The dose response was nearly parallel with that of the bafilomycin A₁-induced lysosomal pH increase. The degree of pH increase for growth inhibition produced by bafilomycin A₁ was similar to that produced by NH₄Cl in which little difference was recognized in effect among cell types.

Original language	English
Pages (from-to)	862-866
Number of pages	5
Journal	In Vitro Cellular & Developmental Biology - Animal: Journal of the Society for In Vitro Biology
Volume	29
Issue number	11
DOIs	https://doi.org/10.1007/BF02631364
Publication status	Published - 1993 Nov
Externally published	Yes

Keywords

NHCl
bafilomycin A
cell growth
lysosomal pH
transformation
vacuolar H-ATPase

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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10.1007/BF02631364

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Inhibition of cell growth by bafilomycin A₁, a selective inhibitor of vacuolar H⁺-ATPase. / Ohkuma, Shoji; Shimizu, Sakae; Noto, Masahiko; Sai, Yoshimichi; Kinoshita, Kuninori; Tamura, Hiro Omi.

In: In Vitro Cellular & Developmental Biology - Animal: Journal of the Society for In Vitro Biology, Vol. 29, No. 11, 11.1993, p. 862-866.

Research output: Contribution to journal › Article › peer-review

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