Inhibition of cell growth by bafilomycin A1, a selective inhibitor of vacuolar H+-ATPase

S. Ohkuma, S. Shimizu, M. Noto, Y. Sai, K. Kinoshita, Hiroomi Tamura

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Bafilomycin A1, a potent selective inhibitor of vacuolar H+-ATPase, inhibited the growth of a variety of cultured cells dose-dependently, including golden hamster embryo and NIH-3T3 fibroblasts, whether or not they were transformed, and PC12 and HeLa cells. The concentration of bafilomycin A1 for 50% inhibition of cell growth ranged from 10 to 50 nM. The dose response was nearly parallel with that of the bafilomycin A1-induced lysosomal pH increase. The degree of pH increase for growth inhibition produced by bafilomycin A1 was similar to that produced by NH4Cl in which little difference was recognized in effect among cell types.

Original languageEnglish
Pages (from-to)862-866
Number of pages5
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume29 A
Issue number11
Publication statusPublished - 1993
Externally publishedYes

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Vacuolar Proton-Translocating ATPases
Cell growth
Growth
PC12 Cells
Mesocricetus
Fibroblasts
HeLa Cells
Cultured Cells
Embryonic Structures
Cells
bafilomycin A1

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Developmental Biology

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Inhibition of cell growth by bafilomycin A1, a selective inhibitor of vacuolar H+-ATPase. / Ohkuma, S.; Shimizu, S.; Noto, M.; Sai, Y.; Kinoshita, K.; Tamura, Hiroomi.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 29 A, No. 11, 1993, p. 862-866.

Research output: Contribution to journalArticle

Ohkuma, S. ; Shimizu, S. ; Noto, M. ; Sai, Y. ; Kinoshita, K. ; Tamura, Hiroomi. / Inhibition of cell growth by bafilomycin A1, a selective inhibitor of vacuolar H+-ATPase. In: In Vitro Cellular and Developmental Biology - Animal. 1993 ; Vol. 29 A, No. 11. pp. 862-866.
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