Abstract
Bafilomycin A1, a potent selective inhibitor of vacuolar H+-ATPase, inhibited the growth of a variety of cultured cells dose-dependently, including golden hamster embryo and NIH-3T3 fibroblasts, whether or not they were transformed, and PC12 and HeLa cells. The concentration of bafilomycin A1 for 50% inhibition of cell growth ranged from 10 to 50 nM. The dose response was nearly parallel with that of the bafilomycin A1-induced lysosomal pH increase. The degree of pH increase for growth inhibition produced by bafilomycin A1 was similar to that produced by NH4Cl in which little difference was recognized in effect among cell types.
| Original language | English |
|---|---|
| Pages (from-to) | 862-866 |
| Number of pages | 5 |
| Journal | In Vitro Cellular and Developmental Biology - Animal |
| Volume | 29 A |
| Issue number | 11 |
| Publication status | Published - 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
- Clinical Biochemistry
- Developmental Biology
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Inhibition of cell growth by bafilomycin A1, a selective inhibitor of vacuolar H+-ATPase. / Ohkuma, S.; Shimizu, S.; Noto, M.; Sai, Y.; Kinoshita, K.; Tamura, Hiroomi.
In: In Vitro Cellular and Developmental Biology - Animal, Vol. 29 A, No. 11, 1993, p. 862-866.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition of cell growth by bafilomycin A1, a selective inhibitor of vacuolar H+-ATPase
AU - Ohkuma, S.
AU - Shimizu, S.
AU - Noto, M.
AU - Sai, Y.
AU - Kinoshita, K.
AU - Tamura, Hiroomi
PY - 1993
Y1 - 1993
N2 - Bafilomycin A1, a potent selective inhibitor of vacuolar H+-ATPase, inhibited the growth of a variety of cultured cells dose-dependently, including golden hamster embryo and NIH-3T3 fibroblasts, whether or not they were transformed, and PC12 and HeLa cells. The concentration of bafilomycin A1 for 50% inhibition of cell growth ranged from 10 to 50 nM. The dose response was nearly parallel with that of the bafilomycin A1-induced lysosomal pH increase. The degree of pH increase for growth inhibition produced by bafilomycin A1 was similar to that produced by NH4Cl in which little difference was recognized in effect among cell types.
AB - Bafilomycin A1, a potent selective inhibitor of vacuolar H+-ATPase, inhibited the growth of a variety of cultured cells dose-dependently, including golden hamster embryo and NIH-3T3 fibroblasts, whether or not they were transformed, and PC12 and HeLa cells. The concentration of bafilomycin A1 for 50% inhibition of cell growth ranged from 10 to 50 nM. The dose response was nearly parallel with that of the bafilomycin A1-induced lysosomal pH increase. The degree of pH increase for growth inhibition produced by bafilomycin A1 was similar to that produced by NH4Cl in which little difference was recognized in effect among cell types.
UR - http://www.scopus.com/inward/record.url?scp=0027882494&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027882494&partnerID=8YFLogxK
M3 - Article
C2 - 8167902
AN - SCOPUS:0027882494
VL - 29 A
SP - 862
EP - 866
JO - In Vitro Cellular & Developmental Biology
JF - In Vitro Cellular & Developmental Biology
SN - 0883-8364
IS - 11
ER -