Inhibition of cellular adhesion in human umbilical vein endothelial cells by NF-κB inhibitor DHMEQ under flow

Osamu Ohno, Yutaka Shima, Yasuo Ikeda, Kumi Sakurai, Kiyoaki Watanabe, Yohko Kawai, Kazuo Umezawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We previously designed and synthesized DHMEQ as an inhibitor of NF-κB. In the present study, we looked into the effect of DHMEQ on the cell adhesion in human umbilical vein endothelial cells (HUVEC) under flow. We used freshly prepared HUVEC and human mononuclear cells throughout the experiment. DHMEQ inhibited TNF-α-, IL-1β-, and LPS-induced NF-κB activation in HUVEC. It also inhibited TNF-α-induced expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin. DHMEQ also inhibited TNF-α-induced mononuclear cell-HUVEC adhesion. The effect of DHMEQ was more prominent when the cells were under shear stress. DHMEQ inhibited the adhesion between HUVEC and HT-29 colon cancer cells more clearly under the flow condition than under the static condition of the culture medium. These results suggest that DHMEQ, being a unique inhibitor of NF-κB, may be effective in suppressing atherosclerosis and metastasis by inhibiting the expression of adhesion molecules.

Original languageEnglish
Pages (from-to)189-197
Number of pages9
JournalOncology research
Volume15
Issue number4
DOIs
Publication statusPublished - 2005 Jan 1

Keywords

  • DHMEQ
  • E-selectin
  • HUVEC
  • ICAM-1
  • NF-κB
  • VCAM-1

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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