Inhibition of effects of flow on potassium permeability in single perfused frog mesenteric capillaries

Mayumi Kajimura, C. C. Michel

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

1. We have investigated the effects of various potential inhibitors on flow-dependent K+ permeability (P(K)) of single perfused mesenteric microvessels in pithed frogs. 2. Neither superfusion with a nitric oxide synthase inhibitor, N(G)-monomethyl-L-arginine (10 or 100 μmol l-1), nor the addition of indomethacin (30 μmol l-1) to both perfusate and superfusate reduced the positive correlation between P(K) and flow velocity (U). 3. In the presence of agents known to raise intracellular levels of adenosine 3',5'-cyclic monophosphate (noradrenaline, 8-bromo-cAMP and a combination of forskolin and rolipram) the slope of the relation between P(K) and U was no longer significant, so that P(K) was no longer flow dependent. 4. These results confirm that the flow dependence of P(K) is a biological process and not an artefact of measurement and suggest a role for intracellular cAMP rather than nitric oxide or prostacyclin in the flow-dependent modulation of P(K) in frog mesenteric microvessels.

Original languageEnglish
Pages (from-to)201-207
Number of pages7
JournalJournal of Physiology
Volume516
Issue number1
Publication statusPublished - 1999 Apr 1
Externally publishedYes

Fingerprint

Microvessels
Anura
Permeability
Potassium
Rolipram
8-Bromo Cyclic Adenosine Monophosphate
Biological Phenomena
Colforsin
Epoprostenol
Nitric Oxide Synthase
Indomethacin
Cyclic AMP
Artifacts
Arginine
Norepinephrine
Nitric Oxide

ASJC Scopus subject areas

  • Physiology

Cite this

Inhibition of effects of flow on potassium permeability in single perfused frog mesenteric capillaries. / Kajimura, Mayumi; Michel, C. C.

In: Journal of Physiology, Vol. 516, No. 1, 01.04.1999, p. 201-207.

Research output: Contribution to journalArticle

@article{afaf6d0f15674431bfcae30904abf57e,
title = "Inhibition of effects of flow on potassium permeability in single perfused frog mesenteric capillaries",
abstract = "1. We have investigated the effects of various potential inhibitors on flow-dependent K+ permeability (P(K)) of single perfused mesenteric microvessels in pithed frogs. 2. Neither superfusion with a nitric oxide synthase inhibitor, N(G)-monomethyl-L-arginine (10 or 100 μmol l-1), nor the addition of indomethacin (30 μmol l-1) to both perfusate and superfusate reduced the positive correlation between P(K) and flow velocity (U). 3. In the presence of agents known to raise intracellular levels of adenosine 3',5'-cyclic monophosphate (noradrenaline, 8-bromo-cAMP and a combination of forskolin and rolipram) the slope of the relation between P(K) and U was no longer significant, so that P(K) was no longer flow dependent. 4. These results confirm that the flow dependence of P(K) is a biological process and not an artefact of measurement and suggest a role for intracellular cAMP rather than nitric oxide or prostacyclin in the flow-dependent modulation of P(K) in frog mesenteric microvessels.",
author = "Mayumi Kajimura and Michel, {C. C.}",
year = "1999",
month = "4",
day = "1",
language = "English",
volume = "516",
pages = "201--207",
journal = "Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Inhibition of effects of flow on potassium permeability in single perfused frog mesenteric capillaries

AU - Kajimura, Mayumi

AU - Michel, C. C.

PY - 1999/4/1

Y1 - 1999/4/1

N2 - 1. We have investigated the effects of various potential inhibitors on flow-dependent K+ permeability (P(K)) of single perfused mesenteric microvessels in pithed frogs. 2. Neither superfusion with a nitric oxide synthase inhibitor, N(G)-monomethyl-L-arginine (10 or 100 μmol l-1), nor the addition of indomethacin (30 μmol l-1) to both perfusate and superfusate reduced the positive correlation between P(K) and flow velocity (U). 3. In the presence of agents known to raise intracellular levels of adenosine 3',5'-cyclic monophosphate (noradrenaline, 8-bromo-cAMP and a combination of forskolin and rolipram) the slope of the relation between P(K) and U was no longer significant, so that P(K) was no longer flow dependent. 4. These results confirm that the flow dependence of P(K) is a biological process and not an artefact of measurement and suggest a role for intracellular cAMP rather than nitric oxide or prostacyclin in the flow-dependent modulation of P(K) in frog mesenteric microvessels.

AB - 1. We have investigated the effects of various potential inhibitors on flow-dependent K+ permeability (P(K)) of single perfused mesenteric microvessels in pithed frogs. 2. Neither superfusion with a nitric oxide synthase inhibitor, N(G)-monomethyl-L-arginine (10 or 100 μmol l-1), nor the addition of indomethacin (30 μmol l-1) to both perfusate and superfusate reduced the positive correlation between P(K) and flow velocity (U). 3. In the presence of agents known to raise intracellular levels of adenosine 3',5'-cyclic monophosphate (noradrenaline, 8-bromo-cAMP and a combination of forskolin and rolipram) the slope of the relation between P(K) and U was no longer significant, so that P(K) was no longer flow dependent. 4. These results confirm that the flow dependence of P(K) is a biological process and not an artefact of measurement and suggest a role for intracellular cAMP rather than nitric oxide or prostacyclin in the flow-dependent modulation of P(K) in frog mesenteric microvessels.

UR - http://www.scopus.com/inward/record.url?scp=0033119167&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033119167&partnerID=8YFLogxK

M3 - Article

C2 - 10066934

AN - SCOPUS:0033119167

VL - 516

SP - 201

EP - 207

JO - Journal of Physiology

JF - Journal of Physiology

SN - 0022-3751

IS - 1

ER -