Abstract
1. We have investigated the effects of various potential inhibitors on flow-dependent K+ permeability (P(K)) of single perfused mesenteric microvessels in pithed frogs. 2. Neither superfusion with a nitric oxide synthase inhibitor, N(G)-monomethyl-L-arginine (10 or 100 μmol l-1), nor the addition of indomethacin (30 μmol l-1) to both perfusate and superfusate reduced the positive correlation between P(K) and flow velocity (U). 3. In the presence of agents known to raise intracellular levels of adenosine 3',5'-cyclic monophosphate (noradrenaline, 8-bromo-cAMP and a combination of forskolin and rolipram) the slope of the relation between P(K) and U was no longer significant, so that P(K) was no longer flow dependent. 4. These results confirm that the flow dependence of P(K) is a biological process and not an artefact of measurement and suggest a role for intracellular cAMP rather than nitric oxide or prostacyclin in the flow-dependent modulation of P(K) in frog mesenteric microvessels.
| Original language | English |
|---|---|
| Pages (from-to) | 201-207 |
| Number of pages | 7 |
| Journal | Journal of Physiology |
| Volume | 516 |
| Issue number | 1 |
| Publication status | Published - 1999 Apr 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Physiology
Cite this
Inhibition of effects of flow on potassium permeability in single perfused frog mesenteric capillaries. / Kajimura, Mayumi; Michel, C. C.
In: Journal of Physiology, Vol. 516, No. 1, 01.04.1999, p. 201-207.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition of effects of flow on potassium permeability in single perfused frog mesenteric capillaries
AU - Kajimura, Mayumi
AU - Michel, C. C.
PY - 1999/4/1
Y1 - 1999/4/1
N2 - 1. We have investigated the effects of various potential inhibitors on flow-dependent K+ permeability (P(K)) of single perfused mesenteric microvessels in pithed frogs. 2. Neither superfusion with a nitric oxide synthase inhibitor, N(G)-monomethyl-L-arginine (10 or 100 μmol l-1), nor the addition of indomethacin (30 μmol l-1) to both perfusate and superfusate reduced the positive correlation between P(K) and flow velocity (U). 3. In the presence of agents known to raise intracellular levels of adenosine 3',5'-cyclic monophosphate (noradrenaline, 8-bromo-cAMP and a combination of forskolin and rolipram) the slope of the relation between P(K) and U was no longer significant, so that P(K) was no longer flow dependent. 4. These results confirm that the flow dependence of P(K) is a biological process and not an artefact of measurement and suggest a role for intracellular cAMP rather than nitric oxide or prostacyclin in the flow-dependent modulation of P(K) in frog mesenteric microvessels.
AB - 1. We have investigated the effects of various potential inhibitors on flow-dependent K+ permeability (P(K)) of single perfused mesenteric microvessels in pithed frogs. 2. Neither superfusion with a nitric oxide synthase inhibitor, N(G)-monomethyl-L-arginine (10 or 100 μmol l-1), nor the addition of indomethacin (30 μmol l-1) to both perfusate and superfusate reduced the positive correlation between P(K) and flow velocity (U). 3. In the presence of agents known to raise intracellular levels of adenosine 3',5'-cyclic monophosphate (noradrenaline, 8-bromo-cAMP and a combination of forskolin and rolipram) the slope of the relation between P(K) and U was no longer significant, so that P(K) was no longer flow dependent. 4. These results confirm that the flow dependence of P(K) is a biological process and not an artefact of measurement and suggest a role for intracellular cAMP rather than nitric oxide or prostacyclin in the flow-dependent modulation of P(K) in frog mesenteric microvessels.
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M3 - Article
C2 - 10066934
AN - SCOPUS:0033119167
VL - 516
SP - 201
EP - 207
JO - Journal of Physiology
JF - Journal of Physiology
SN - 0022-3751
IS - 1
ER -