Inhibition of encystation of Entamoeba invadens by aphidicolin

Masahiro Kumagai, Asao Makioka, Hiroshi Ohtomo, Seiki Kobayashi, Tsutomu Takeuchi

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2 Citations (Scopus)

Abstract

Effects of aphidicolin, a specific inhibitor of eukaryotic nuclear replicative DNA polymerases, on the growth and encystation of a reptilian parasitic protozoan Entamoeba invadens were evaluated. Aphidicolin blocked the growth of axenic E. invadens strain IP-1 in a dose-dependent manner. Encystation induced by the glucose depletion and osmotic pressure was also inhibited by aphidicolin. The inhibition was almost complete when trophozoites were treated with the drug before being transferred to encystation medium containing the drug. The inhibitory effect of aphidicolin on E. invadens growth was abrogated by removal of the drug, and exposure to 3 μg/ml of the drug for at least 7 days had little effect on the viability. Encystation was also restored by removal of the drug. These results suggest that the trophozoites accumulated at G1/S border by treatment with aphidicolin cannot encyst upon induction of encystation so that they need DNA synthesis before encystation.

Original languageEnglish
Pages (from-to)313-317
Number of pages5
JournalTokai Journal of Experimental and Clinical Medicine
Volume23
Issue number6
Publication statusPublished - 1998 Dec 1

Keywords

  • Aphidicolin
  • DNA polymerase
  • Encystation
  • Entamoeba invadens

ASJC Scopus subject areas

  • Medicine(all)

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    Kumagai, M., Makioka, A., Ohtomo, H., Kobayashi, S., & Takeuchi, T. (1998). Inhibition of encystation of Entamoeba invadens by aphidicolin. Tokai Journal of Experimental and Clinical Medicine, 23(6), 313-317.