Inhibition of encystation of Entamoeba invadens by aphidicolin

Masahiro Kumagai, Asao Makioka, Hiroshi Ohtomo, Seiki Kobayashi, Tsutomu Takeuchi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Effects of aphidicolin, a specific inhibitor of eukaryotic nuclear replicative DNA polymerases, on the growth and encystation of a reptilian parasitic protozoan Entamoeba invadens were evaluated. Aphidicolin blocked the growth of axenic E. invadens strain IP-1 in a dose-dependent manner. Encystation induced by the glucose depletion and osmotic pressure was also inhibited by aphidicolin. The inhibition was almost complete when trophozoites were treated with the drug before being transferred to encystation medium containing the drug. The inhibitory effect of aphidicolin on E. invadens growth was abrogated by removal of the drug, and exposure to 3 μg/ml of the drug for at least 7 days had little effect on the viability. Encystation was also restored by removal of the drug. These results suggest that the trophozoites accumulated at G1/S border by treatment with aphidicolin cannot encyst upon induction of encystation so that they need DNA synthesis before encystation.

Original languageEnglish
Pages (from-to)313-317
Number of pages5
JournalTokai Journal of Experimental and Clinical Medicine
Volume23
Issue number6
Publication statusPublished - 1998 Dec

Fingerprint

Entamoeba
Aphidicolin
Trophozoites
Pharmaceutical Preparations
Growth
Osmotic Pressure
DNA-Directed DNA Polymerase
Glucose
DNA

Keywords

  • Aphidicolin
  • DNA polymerase
  • Encystation
  • Entamoeba invadens

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Kumagai, M., Makioka, A., Ohtomo, H., Kobayashi, S., & Takeuchi, T. (1998). Inhibition of encystation of Entamoeba invadens by aphidicolin. Tokai Journal of Experimental and Clinical Medicine, 23(6), 313-317.

Inhibition of encystation of Entamoeba invadens by aphidicolin. / Kumagai, Masahiro; Makioka, Asao; Ohtomo, Hiroshi; Kobayashi, Seiki; Takeuchi, Tsutomu.

In: Tokai Journal of Experimental and Clinical Medicine, Vol. 23, No. 6, 12.1998, p. 313-317.

Research output: Contribution to journalArticle

Kumagai, M, Makioka, A, Ohtomo, H, Kobayashi, S & Takeuchi, T 1998, 'Inhibition of encystation of Entamoeba invadens by aphidicolin', Tokai Journal of Experimental and Clinical Medicine, vol. 23, no. 6, pp. 313-317.
Kumagai M, Makioka A, Ohtomo H, Kobayashi S, Takeuchi T. Inhibition of encystation of Entamoeba invadens by aphidicolin. Tokai Journal of Experimental and Clinical Medicine. 1998 Dec;23(6):313-317.
Kumagai, Masahiro ; Makioka, Asao ; Ohtomo, Hiroshi ; Kobayashi, Seiki ; Takeuchi, Tsutomu. / Inhibition of encystation of Entamoeba invadens by aphidicolin. In: Tokai Journal of Experimental and Clinical Medicine. 1998 ; Vol. 23, No. 6. pp. 313-317.
@article{0ad6fcb20f2d4c7a8f0628c5264aa894,
title = "Inhibition of encystation of Entamoeba invadens by aphidicolin",
abstract = "Effects of aphidicolin, a specific inhibitor of eukaryotic nuclear replicative DNA polymerases, on the growth and encystation of a reptilian parasitic protozoan Entamoeba invadens were evaluated. Aphidicolin blocked the growth of axenic E. invadens strain IP-1 in a dose-dependent manner. Encystation induced by the glucose depletion and osmotic pressure was also inhibited by aphidicolin. The inhibition was almost complete when trophozoites were treated with the drug before being transferred to encystation medium containing the drug. The inhibitory effect of aphidicolin on E. invadens growth was abrogated by removal of the drug, and exposure to 3 μg/ml of the drug for at least 7 days had little effect on the viability. Encystation was also restored by removal of the drug. These results suggest that the trophozoites accumulated at G1/S border by treatment with aphidicolin cannot encyst upon induction of encystation so that they need DNA synthesis before encystation.",
keywords = "Aphidicolin, DNA polymerase, Encystation, Entamoeba invadens",
author = "Masahiro Kumagai and Asao Makioka and Hiroshi Ohtomo and Seiki Kobayashi and Tsutomu Takeuchi",
year = "1998",
month = "12",
language = "English",
volume = "23",
pages = "313--317",
journal = "Tokai Journal of Experimental and Clinical Medicine",
issn = "0385-0005",
publisher = "Tokai Medical Association",
number = "6",

}

TY - JOUR

T1 - Inhibition of encystation of Entamoeba invadens by aphidicolin

AU - Kumagai, Masahiro

AU - Makioka, Asao

AU - Ohtomo, Hiroshi

AU - Kobayashi, Seiki

AU - Takeuchi, Tsutomu

PY - 1998/12

Y1 - 1998/12

N2 - Effects of aphidicolin, a specific inhibitor of eukaryotic nuclear replicative DNA polymerases, on the growth and encystation of a reptilian parasitic protozoan Entamoeba invadens were evaluated. Aphidicolin blocked the growth of axenic E. invadens strain IP-1 in a dose-dependent manner. Encystation induced by the glucose depletion and osmotic pressure was also inhibited by aphidicolin. The inhibition was almost complete when trophozoites were treated with the drug before being transferred to encystation medium containing the drug. The inhibitory effect of aphidicolin on E. invadens growth was abrogated by removal of the drug, and exposure to 3 μg/ml of the drug for at least 7 days had little effect on the viability. Encystation was also restored by removal of the drug. These results suggest that the trophozoites accumulated at G1/S border by treatment with aphidicolin cannot encyst upon induction of encystation so that they need DNA synthesis before encystation.

AB - Effects of aphidicolin, a specific inhibitor of eukaryotic nuclear replicative DNA polymerases, on the growth and encystation of a reptilian parasitic protozoan Entamoeba invadens were evaluated. Aphidicolin blocked the growth of axenic E. invadens strain IP-1 in a dose-dependent manner. Encystation induced by the glucose depletion and osmotic pressure was also inhibited by aphidicolin. The inhibition was almost complete when trophozoites were treated with the drug before being transferred to encystation medium containing the drug. The inhibitory effect of aphidicolin on E. invadens growth was abrogated by removal of the drug, and exposure to 3 μg/ml of the drug for at least 7 days had little effect on the viability. Encystation was also restored by removal of the drug. These results suggest that the trophozoites accumulated at G1/S border by treatment with aphidicolin cannot encyst upon induction of encystation so that they need DNA synthesis before encystation.

KW - Aphidicolin

KW - DNA polymerase

KW - Encystation

KW - Entamoeba invadens

UR - http://www.scopus.com/inward/record.url?scp=0032321756&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032321756&partnerID=8YFLogxK

M3 - Article

VL - 23

SP - 313

EP - 317

JO - Tokai Journal of Experimental and Clinical Medicine

JF - Tokai Journal of Experimental and Clinical Medicine

SN - 0385-0005

IS - 6

ER -