Inhibition of glioma cell proliferation by neural stem cell factor

Tsuyoshi Suzuki, Shuichi Izumoto, Kouichi Wada, Yasunori Fujimoto, Motohiko Maruno, Mami Yamasaki, Yonehiro Kanemura, Takuya Shimazaki, Hideyuki Okano, Toshiki Yoshimine

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Neural stem cells (NSC) have unique differentiation-, proliferation-, and motility properties. To investigate whether they secrete factors that interfere with the proliferation of glioma cells, we grew glioma cells in conditioned medium (CM) obtained from cultures of neurospheres including neural stem/progenitor cells (NSPC) isolated from embryonic (E14)- or adult mouse brain or fetal human brain. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and BrdU-labeling assays showed that CM from NSPC (NSPC/CM) contained factor(s) that inhibited the proliferation of glioma cells by 28-87%. Filter-fractionation of NSPC/CM revealed that the 50,000-100,000 nominal molecular weight limit (NMWL) fraction contained the inhibitory activity. On the basis of these observations we transplanted 203G glioma cells and/or NSPC into the intrathecal space of the cisterna magna of mice to investigate whether NSPC interfere with the proliferation of glioma cells in vivo. Mice transplanted with both 203G and NSPC survived significantly longer than did mice transplanted only with 203G. We concluded that NSPC secrete factor(s) that may control glioma cell proliferation.

Original languageEnglish
Pages (from-to)233-239
Number of pages7
JournalJournal of Neuro-Oncology
Volume74
Issue number3
DOIs
Publication statusPublished - 2005 Sep

Fingerprint

Stem Cell Factor
Neural Stem Cells
Glioma
Cell Proliferation
Stem Cells
Conditioned Culture Medium
Cisterna Magna
Brain
Bromodeoxyuridine
Molecular Weight

Keywords

  • Cell proliferation
  • Glioma
  • Glioma cell
  • Neural progenitor cell
  • Neural stem cell

ASJC Scopus subject areas

  • Clinical Neurology
  • Cancer Research
  • Oncology
  • Neuroscience(all)

Cite this

Suzuki, T., Izumoto, S., Wada, K., Fujimoto, Y., Maruno, M., Yamasaki, M., ... Yoshimine, T. (2005). Inhibition of glioma cell proliferation by neural stem cell factor. Journal of Neuro-Oncology, 74(3), 233-239. https://doi.org/10.1007/s11060-004-7118-5

Inhibition of glioma cell proliferation by neural stem cell factor. / Suzuki, Tsuyoshi; Izumoto, Shuichi; Wada, Kouichi; Fujimoto, Yasunori; Maruno, Motohiko; Yamasaki, Mami; Kanemura, Yonehiro; Shimazaki, Takuya; Okano, Hideyuki; Yoshimine, Toshiki.

In: Journal of Neuro-Oncology, Vol. 74, No. 3, 09.2005, p. 233-239.

Research output: Contribution to journalArticle

Suzuki, T, Izumoto, S, Wada, K, Fujimoto, Y, Maruno, M, Yamasaki, M, Kanemura, Y, Shimazaki, T, Okano, H & Yoshimine, T 2005, 'Inhibition of glioma cell proliferation by neural stem cell factor', Journal of Neuro-Oncology, vol. 74, no. 3, pp. 233-239. https://doi.org/10.1007/s11060-004-7118-5
Suzuki T, Izumoto S, Wada K, Fujimoto Y, Maruno M, Yamasaki M et al. Inhibition of glioma cell proliferation by neural stem cell factor. Journal of Neuro-Oncology. 2005 Sep;74(3):233-239. https://doi.org/10.1007/s11060-004-7118-5
Suzuki, Tsuyoshi ; Izumoto, Shuichi ; Wada, Kouichi ; Fujimoto, Yasunori ; Maruno, Motohiko ; Yamasaki, Mami ; Kanemura, Yonehiro ; Shimazaki, Takuya ; Okano, Hideyuki ; Yoshimine, Toshiki. / Inhibition of glioma cell proliferation by neural stem cell factor. In: Journal of Neuro-Oncology. 2005 ; Vol. 74, No. 3. pp. 233-239.
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