Inhibition of heat-shock protein 27 reduces 5-Fluorouracil-acquired resistance in human colon cancer cells

Yusuke Asada, Masashi Tsuruta, Koji Okabayashi, Kohei Shigeta, Takashi Ishida, Takehiro Shimada, Hirofumi Suzumura, Kaoru Koishikawa, Shingo Akimoto, Hirotoshi Hasegawa, Yuko Kitagawa

Research output: Contribution to journalArticlepeer-review

Abstract

Background/Aim: In previous work we showed that expression of heat-shock protein 27 (HSP27; encoded by HSPB1) was associated with inherent resistance to 5-fluorouracil (5-FU). However, the relationship between HSP27 and acquired resistance remains unknown. Materials and Methods: We generated an acquired resistance model (WiDr-R) of a colon cancer cell line by exposing WiDr cells to 5-FU. Cell viability assays under treatment with 5-FU, as well as down-regulation of HSP27 using small interfering HSP27 RNA, were performed. HSP27 mRNA and protein expression was analyzed using real-time polymerase chain reaction and western blotting. Results: 5-FU-acquired resistance induced overexpression of HSP27 mRNA and protein levels in WiDr-R cells. Furthermore, siRNA knockdown of HSP27 in WiDr-R cells reduced 5-FU-acquired resistance. Conclusion: These findings demonstrate that HSP27 is associated with 5-FU resistance in human colon cancer cell cells and suggest that HSP27 regulation represents a novel approach to overcoming chemoresistance in colorectal cancer.

Original languageEnglish
Pages (from-to)1283-1290
Number of pages8
JournalAnticancer research
Volume41
Issue number3
DOIs
Publication statusPublished - 2021 Mar

Keywords

  • 5-fluorouracil resistance
  • Chemotherapy
  • Colorectal cancer
  • Heat-shock protein 27

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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