Inhibition of IGF-1-Mediated cellular migration and invasion by migracin a in ovarian clear cell carcinoma cells

Tamami Ukaji, Yinzhi Lin, Kouji Banno, Shoshiro Okada, Kazuo Umezawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Previously we isolated migracin A from a Streptomyces culture filtrate as an inhibitor of cancer cell migration. In the present research, we found that migracin A inhibited migration and invasion of ovarian clear cell carcinoma ES-2 cells. In the course of our mechanistic study, migracin A was shown to enhance vasohibin-1 expression in an angiogenesis array. We also confirmed that it increased the mRNA expression of this protein. Moreover, overexpression of vasohibin-1 lowered the migration but not the invasion of ES-2 cells. Then, we looked for another target protein employing a motility array, and found that migracin A lowered the IGF-1 expression. Knockdown of IGF-1 by siRNA decreased the migration and invasion of ES-2 cells. Migracin A also decreased Akt phosphorylation involved in the downstream signaling. Crosstalk analysis indicated that overexpression of vasohibin-1 decreased the IGF-1 expression. On the other hand, it showed no direct anticancer activity in terms of the ES-2 growth in agar. Migracin A inhibited the migration and IGF-1 expression in not only ES-2 but also another ovarian clear cell carcinoma JHOC-5 cells. In addition, it also inhibited capillary tube formation of human umbilical vein endothelial cells. Since its cytotoxicity is very low, migracin A may be a candidate for an anti-metastasis agent not exhibiting prominent toxicity.

Original languageEnglish
Article numbere0137663
JournalPLoS One
Volume10
Issue number9
DOIs
Publication statusPublished - 2015 Sep 11

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Insulin-Like Growth Factor I
cell movement
carcinoma
Cells
Carcinoma
cells
molecular motor proteins
Streptomyces
culture filtrates
Capillary tubes
Phosphorylation
small interfering RNA
angiogenesis
metastasis
Endothelial cells
Human Umbilical Vein Endothelial Cells
Cytotoxicity
Crosstalk
migracin A
cytotoxicity

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Inhibition of IGF-1-Mediated cellular migration and invasion by migracin a in ovarian clear cell carcinoma cells. / Ukaji, Tamami; Lin, Yinzhi; Banno, Kouji; Okada, Shoshiro; Umezawa, Kazuo.

In: PLoS One, Vol. 10, No. 9, e0137663, 11.09.2015.

Research output: Contribution to journalArticle

Ukaji, Tamami ; Lin, Yinzhi ; Banno, Kouji ; Okada, Shoshiro ; Umezawa, Kazuo. / Inhibition of IGF-1-Mediated cellular migration and invasion by migracin a in ovarian clear cell carcinoma cells. In: PLoS One. 2015 ; Vol. 10, No. 9.
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