Inhibition of lysenin-induced hemolysis by All -E -Lutein derived from the plant dalbergia latifolia

Yuki Niwa, Chino Matsui, Neelanun Sukumwang, Hironobu Iinuma, Yoko Ikeda, Takashi Koyano, Taworn Kovitayakorn, Siro Simizu, Kazuo Umezawa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Lysenin is a pore-forming toxin derived from coelomic fluid of the earthworm Eisenia foetida. The model of lysenin-induced hemolysis includes the specific binding of lysenin to sphingomyelin, oligomerization of the pore proteins, and pore formation. Although the mechanism of lysenin-induced hemolysis is unique, its precise mechanism of action and its inhibitors are poorly understood. In the present study, we screened for inhibitors of lysenin-induced hemolysis by using an optimized screening system and found a methanolic extract of Dalbergia latifolia leaves to be a potential candidate. After isolation and identification, all-E-lutein was identified as the hemolysis inhibitor with an effective dose of 0.025-2.5 ng/mL without any toxicity. The inhibition by all-E-lutein is likely to occur during the receptor binding and/or pore-forming protein oligomerization.

Original languageEnglish
Pages (from-to)957-961
Number of pages5
JournalPlanta Medica
Volume78
Issue number10
DOIs
Publication statusPublished - 2012

Fingerprint

Dalbergia
Lutein
Hemolysis
Porins
Oligochaeta
Oligomerization
Sphingomyelins
Toxicity
Screening
lysenin
Fluids
Proteins

Keywords

  • all- E- lutein
  • Dalbergia latifolia
  • Fabaceae
  • hemolysis
  • Indian rosewood
  • Leguminosae
  • lysenin

ASJC Scopus subject areas

  • Complementary and alternative medicine
  • Molecular Medicine
  • Organic Chemistry
  • Analytical Chemistry
  • Pharmaceutical Science
  • Pharmacology
  • Drug Discovery

Cite this

Niwa, Y., Matsui, C., Sukumwang, N., Iinuma, H., Ikeda, Y., Koyano, T., ... Umezawa, K. (2012). Inhibition of lysenin-induced hemolysis by All -E -Lutein derived from the plant dalbergia latifolia. Planta Medica, 78(10), 957-961. https://doi.org/10.1055/s-0031-1298538

Inhibition of lysenin-induced hemolysis by All -E -Lutein derived from the plant dalbergia latifolia. / Niwa, Yuki; Matsui, Chino; Sukumwang, Neelanun; Iinuma, Hironobu; Ikeda, Yoko; Koyano, Takashi; Kovitayakorn, Taworn; Simizu, Siro; Umezawa, Kazuo.

In: Planta Medica, Vol. 78, No. 10, 2012, p. 957-961.

Research output: Contribution to journalArticle

Niwa, Y, Matsui, C, Sukumwang, N, Iinuma, H, Ikeda, Y, Koyano, T, Kovitayakorn, T, Simizu, S & Umezawa, K 2012, 'Inhibition of lysenin-induced hemolysis by All -E -Lutein derived from the plant dalbergia latifolia', Planta Medica, vol. 78, no. 10, pp. 957-961. https://doi.org/10.1055/s-0031-1298538
Niwa, Yuki ; Matsui, Chino ; Sukumwang, Neelanun ; Iinuma, Hironobu ; Ikeda, Yoko ; Koyano, Takashi ; Kovitayakorn, Taworn ; Simizu, Siro ; Umezawa, Kazuo. / Inhibition of lysenin-induced hemolysis by All -E -Lutein derived from the plant dalbergia latifolia. In: Planta Medica. 2012 ; Vol. 78, No. 10. pp. 957-961.
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