Inhibition of lysenin-induced hemolysis by All -E -Lutein derived from the plant dalbergia latifolia

Yuki Niwa, Chino Matsui, Neelanun Sukumwang, Hironobu Iinuma, Yoko Ikeda, Takashi Koyano, Taworn Kovitayakorn, Siro Simizu, Kazuo Umezawa

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1 Citation (Scopus)

Abstract

Lysenin is a pore-forming toxin derived from coelomic fluid of the earthworm Eisenia foetida. The model of lysenin-induced hemolysis includes the specific binding of lysenin to sphingomyelin, oligomerization of the pore proteins, and pore formation. Although the mechanism of lysenin-induced hemolysis is unique, its precise mechanism of action and its inhibitors are poorly understood. In the present study, we screened for inhibitors of lysenin-induced hemolysis by using an optimized screening system and found a methanolic extract of Dalbergia latifolia leaves to be a potential candidate. After isolation and identification, all-E-lutein was identified as the hemolysis inhibitor with an effective dose of 0.025-2.5 ng/mL without any toxicity. The inhibition by all-E-lutein is likely to occur during the receptor binding and/or pore-forming protein oligomerization.

Original languageEnglish
Pages (from-to)957-961
Number of pages5
JournalPlanta Medica
Volume78
Issue number10
DOIs
Publication statusPublished - 2012 May 16

Keywords

  • Dalbergia latifolia
  • Fabaceae
  • Indian rosewood
  • Leguminosae
  • all- E- lutein
  • hemolysis
  • lysenin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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  • Cite this

    Niwa, Y., Matsui, C., Sukumwang, N., Iinuma, H., Ikeda, Y., Koyano, T., Kovitayakorn, T., Simizu, S., & Umezawa, K. (2012). Inhibition of lysenin-induced hemolysis by All -E -Lutein derived from the plant dalbergia latifolia. Planta Medica, 78(10), 957-961. https://doi.org/10.1055/s-0031-1298538