Inhibition of MMP-2-mediated mast cell invasion by NF-κB inhibitor DHMEQ in mast cells

Naruto Noma, Masataka Asagiri, Masatoshi Takeiri, Saori Ohmae, Kenji Takemoto, Keiko Iwaisako, Nagahiro Minato, Mari Maeda-Yamamoto, Siro Simizu, Kazuo Umezawa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Stimulation with antigen and IgE is known to activate NF-κB in mast cells. In the present research, we studied the role of NF-κB on cellular migration in mast cell-like RBL-2H3 cells and bone marrow-derived mast cells (BMMCs) using the NF-κB inhibitor (-)-DHMEQ. Methods: A Matrigel invasion chamber was used to evaluate cell migration. A PCR array was used to screen the expression of 84 key genes involved in cell migration. Results: (-)-DHMEQ inhibited antigen/IgE-induced NF-κB activation and expressions of its target genes such as IL-6 and TNF-α. (-)-DHMEQ was found to inhibit in vitro invasion toward the antigen without any toxicity. We then looked for NF-κB-dependent genes that would be important for mast cell invasion using the PCR array. (-)-DHMEQ was found to lower the expression of matrix metalloproteinase (MMP)-2. The MMP inhibitor GM6001 also inhibited cellular invasion toward the antigen. These effects of (-)-DHMEQ were obtained in both RBL-2H3 cells and BMMCs. Conclusions: These findings indicate that (-)-DHMEQ suppressed mast cell migration via the inhibition of NF-κB-regulated MMP-2 expression.

Original languageEnglish
Pages (from-to)84-90
Number of pages7
JournalInternational Archives of Allergy and Immunology
Volume166
Issue number2
DOIs
Publication statusPublished - 2015 May 6

Keywords

  • (-)-DHMEQ
  • Invasion
  • MMP-2
  • Mast cell
  • NF-κB

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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  • Cite this

    Noma, N., Asagiri, M., Takeiri, M., Ohmae, S., Takemoto, K., Iwaisako, K., Minato, N., Maeda-Yamamoto, M., Simizu, S., & Umezawa, K. (2015). Inhibition of MMP-2-mediated mast cell invasion by NF-κB inhibitor DHMEQ in mast cells. International Archives of Allergy and Immunology, 166(2), 84-90. https://doi.org/10.1159/000371419