Inhibition of MMP-2-mediated mast cell invasion by NF-κB inhibitor DHMEQ in mast cells

Naruto Noma, Masataka Asagiri, Masatoshi Takeiri, Saori Ohmae, Kenji Takemoto, Keiko Iwaisako, Nagahiro Minato, Mari Maeda-Yamamoto, Siro Simizu, Kazuo Umezawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Stimulation with antigen and IgE is known to activate NF-κB in mast cells. In the present research, we studied the role of NF-κB on cellular migration in mast cell-like RBL-2H3 cells and bone marrow-derived mast cells (BMMCs) using the NF-κB inhibitor (-)-DHMEQ. Methods: A Matrigel invasion chamber was used to evaluate cell migration. A PCR array was used to screen the expression of 84 key genes involved in cell migration. Results: (-)-DHMEQ inhibited antigen/IgE-induced NF-κB activation and expressions of its target genes such as IL-6 and TNF-α. (-)-DHMEQ was found to inhibit in vitro invasion toward the antigen without any toxicity. We then looked for NF-κB-dependent genes that would be important for mast cell invasion using the PCR array. (-)-DHMEQ was found to lower the expression of matrix metalloproteinase (MMP)-2. The MMP inhibitor GM6001 also inhibited cellular invasion toward the antigen. These effects of (-)-DHMEQ were obtained in both RBL-2H3 cells and BMMCs. Conclusions: These findings indicate that (-)-DHMEQ suppressed mast cell migration via the inhibition of NF-κB-regulated MMP-2 expression.

Original languageEnglish
Pages (from-to)84-90
Number of pages7
JournalInternational Archives of Allergy and Immunology
Volume166
Issue number2
DOIs
Publication statusPublished - 2015 May 6

Fingerprint

Matrix Metalloproteinase 2
Mast Cells
Antigens
Bone Marrow Cells
Immunoglobulin E
Cell Movement
Cell Migration Inhibition
Genes
Polymerase Chain Reaction
Matrix Metalloproteinase Inhibitors
Interleukin-6
Research

Keywords

  • (-)-DHMEQ
  • Invasion
  • Mast cell
  • MMP-2
  • NF-κB

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Noma, N., Asagiri, M., Takeiri, M., Ohmae, S., Takemoto, K., Iwaisako, K., ... Umezawa, K. (2015). Inhibition of MMP-2-mediated mast cell invasion by NF-κB inhibitor DHMEQ in mast cells. International Archives of Allergy and Immunology, 166(2), 84-90. https://doi.org/10.1159/000371419

Inhibition of MMP-2-mediated mast cell invasion by NF-κB inhibitor DHMEQ in mast cells. / Noma, Naruto; Asagiri, Masataka; Takeiri, Masatoshi; Ohmae, Saori; Takemoto, Kenji; Iwaisako, Keiko; Minato, Nagahiro; Maeda-Yamamoto, Mari; Simizu, Siro; Umezawa, Kazuo.

In: International Archives of Allergy and Immunology, Vol. 166, No. 2, 06.05.2015, p. 84-90.

Research output: Contribution to journalArticle

Noma, N, Asagiri, M, Takeiri, M, Ohmae, S, Takemoto, K, Iwaisako, K, Minato, N, Maeda-Yamamoto, M, Simizu, S & Umezawa, K 2015, 'Inhibition of MMP-2-mediated mast cell invasion by NF-κB inhibitor DHMEQ in mast cells', International Archives of Allergy and Immunology, vol. 166, no. 2, pp. 84-90. https://doi.org/10.1159/000371419
Noma, Naruto ; Asagiri, Masataka ; Takeiri, Masatoshi ; Ohmae, Saori ; Takemoto, Kenji ; Iwaisako, Keiko ; Minato, Nagahiro ; Maeda-Yamamoto, Mari ; Simizu, Siro ; Umezawa, Kazuo. / Inhibition of MMP-2-mediated mast cell invasion by NF-κB inhibitor DHMEQ in mast cells. In: International Archives of Allergy and Immunology. 2015 ; Vol. 166, No. 2. pp. 84-90.
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AU - Takemoto, Kenji

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AU - Minato, Nagahiro

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