Inhibition of mouse epidermal 12-lipoxygenase by 2,3,4-trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1,4-benzoquinone (AA861)

T. Nakadate, S. Yamamoto, Eriko Yokota, R. Kato

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

2,3,5-Trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1,4-benzoquinone (AA861) strongly inhibited epidermal lipoxygenase activity which was determined by the formation of [14C]12-hydroxy-5,8,10,14-eicosatetraenoic acid by incubating [14C]arachidonic acid with cytosol fraction of epidermal homogenate of CD-1 mice. AA861 failed to inhibit epidermal cyclooxygenase activity. The present results indicate that AA861 is a potent inhibitor of 12-lipoxygenase in mouse epidermis (IC50 1.9 μM).

Original languageEnglish
Pages (from-to)71-73
Number of pages3
JournalJournal of Pharmacy and Pharmacology
Volume37
Issue number1
Publication statusPublished - 1985

Fingerprint

Arachidonate 12-Lipoxygenase
Lipoxygenase
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Lipoxygenase Inhibitors
Prostaglandin-Endoperoxide Synthases
Arachidonic Acid
Epidermis
Cytosol
Inhibitory Concentration 50
lipoxygenase 3
benzoquinone
2,3,5-trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1,4-benzoquinone

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

Inhibition of mouse epidermal 12-lipoxygenase by 2,3,4-trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1,4-benzoquinone (AA861). / Nakadate, T.; Yamamoto, S.; Yokota, Eriko; Kato, R.

In: Journal of Pharmacy and Pharmacology, Vol. 37, No. 1, 1985, p. 71-73.

Research output: Contribution to journalArticle

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AU - Yokota, Eriko

AU - Kato, R.

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AB - 2,3,5-Trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1,4-benzoquinone (AA861) strongly inhibited epidermal lipoxygenase activity which was determined by the formation of [14C]12-hydroxy-5,8,10,14-eicosatetraenoic acid by incubating [14C]arachidonic acid with cytosol fraction of epidermal homogenate of CD-1 mice. AA861 failed to inhibit epidermal cyclooxygenase activity. The present results indicate that AA861 is a potent inhibitor of 12-lipoxygenase in mouse epidermis (IC50 1.9 μM).

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