Inhibition of septation in bacillus subtilis by a peptide antibiotic, edeine B1

Kumiko W. Shimotohno, Fujio Kawamura, Yousuke Natori, Hideaki Nanamiya, Junji Magae, Hiromitsu Ogata, Toyoshige Endo, Takeshi Suzuki, Hiroshi Yamaki

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A peptide antibiotic, edeine B1, exerts a lethal action in Bacillus subtilis causing filamentous morphology. This antibiotic assumes to inhibit cell division by interacting with FtsZ and inhibiting FtsZ polymerization. The temperature-sensitive mutant ftsZ ts1 was shown to be hypersensitive to the antibiotic as compared to the parent 168 with respect to its lethal action and the sensitivity to the antibiotic of the revertant of ftsZ ts1 was shown to be intermediate between those of the parent 168 and the ftsZ ts1. Alteration of FtsZ sequence may be responsible for sensitivity to edeine B1. The residues at 240, 278, 345 and 346 in the FtsZ sequence of the parent 168 were A240, A278, D345 and A346. Those of ftsZ ts1 were V240, V278, E345 and P346. Those of the revertant of ftsZ ts1 were A240, A278, E345 and P346. The difference in sensitivity to edeine B1 among these strains is presumably due to the difference in the residues at 240, 278, 345 and 346 in the FtsZ sequence. The sequential events of the inhibition of FtsZ assembly and the inhibition of protein biosynthesis by edeine B1 may progress synergistically, resulting in cell death.

Original languageEnglish
Pages (from-to)568-571
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume33
Issue number4
DOIs
Publication statusPublished - 2010 Apr 10

Fingerprint

Bacillus subtilis
Anti-Bacterial Agents
Peptides
Protein Biosynthesis
Polymerization
Cell Division
Cell Death
Temperature
edeine B

Keywords

  • Edeine B
  • Ftsz
  • Inhibition
  • Septation

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

Shimotohno, K. W., Kawamura, F., Natori, Y., Nanamiya, H., Magae, J., Ogata, H., ... Yamaki, H. (2010). Inhibition of septation in bacillus subtilis by a peptide antibiotic, edeine B1 Biological and Pharmaceutical Bulletin, 33(4), 568-571. https://doi.org/10.1248/bpb.33.568

Inhibition of septation in bacillus subtilis by a peptide antibiotic, edeine B1 . / Shimotohno, Kumiko W.; Kawamura, Fujio; Natori, Yousuke; Nanamiya, Hideaki; Magae, Junji; Ogata, Hiromitsu; Endo, Toyoshige; Suzuki, Takeshi; Yamaki, Hiroshi.

In: Biological and Pharmaceutical Bulletin, Vol. 33, No. 4, 10.04.2010, p. 568-571.

Research output: Contribution to journalArticle

Shimotohno, KW, Kawamura, F, Natori, Y, Nanamiya, H, Magae, J, Ogata, H, Endo, T, Suzuki, T & Yamaki, H 2010, 'Inhibition of septation in bacillus subtilis by a peptide antibiotic, edeine B1 ', Biological and Pharmaceutical Bulletin, vol. 33, no. 4, pp. 568-571. https://doi.org/10.1248/bpb.33.568
Shimotohno KW, Kawamura F, Natori Y, Nanamiya H, Magae J, Ogata H et al. Inhibition of septation in bacillus subtilis by a peptide antibiotic, edeine B1 Biological and Pharmaceutical Bulletin. 2010 Apr 10;33(4):568-571. https://doi.org/10.1248/bpb.33.568
Shimotohno, Kumiko W. ; Kawamura, Fujio ; Natori, Yousuke ; Nanamiya, Hideaki ; Magae, Junji ; Ogata, Hiromitsu ; Endo, Toyoshige ; Suzuki, Takeshi ; Yamaki, Hiroshi. / Inhibition of septation in bacillus subtilis by a peptide antibiotic, edeine B1 In: Biological and Pharmaceutical Bulletin. 2010 ; Vol. 33, No. 4. pp. 568-571.
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