Inhibition of Transcription by Mammalian Ribonucleic Acid Polymerase II: Effects of Diethylstilbestrol and Its Analogues

Taiko Oda, Yoshihiro Sato, Shigetaka Kitajima, Yukio Yasukochi

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2 Citations (Scopus)

Abstract

We examined the effects of diethylstilbestrol (1), Z,Z-dienestrol (2), E,E-dienestrol (3), indenestrol A (4), indenestrol B (5) and estradiol (6) on the activity of in vitro accurate transcription from adenovirus 2 major late promoter by mammalian ribonucleic acid (RNA) polymerase II using partially purified transcription factors from HeLa cell nuclear extract as well as on RNA polymerase II activity assayed for random incorporation of ribonucleotides. 1, 2, and 3 inhibited both activities, whereas 4 and 5 were inhibitory only in the transcription activity at concentrations higher than 10/ig/ml. However 6 had no effects on both activities.

Original languageEnglish
Pages (from-to)2627-2629
Number of pages3
JournalChemical and Pharmaceutical Bulletin
Volume39
Issue number10
DOIs
Publication statusPublished - 1991 Jan 1

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Keywords

  • RNA polymerase II
  • dienestrol
  • diethylstilbestrol
  • estradiol
  • indenestrol A
  • indenestrol B
  • transcription

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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