Abstract
The effects of Rikkunshi-To on gastric mucosal injury were investigated with attention to the mechanisms of oxidative stress. Repeated electrical stimulation (RES) on the gastric artery of male Wistar rats decreased mucosal blood flow, which was measured by laser Doppler flowmetry, and subsequently produced hemorrhagic lesions in the gastric mucosa. At 30 min after the completion of RES, increased platelet-activating factor (PAF) content and myeloperoxidase (MPO) activity were observed in the gastric mucosa. Luminol-dependent chemiluminescence activity in blood samples taken from a gastric vein was also elevated after the stimulation. Oral administration of Rikkunshi-To (50 mg/kg) attenuated the increases in luminol-dependent chemiluminescence activity, mucosal PAF content and MPO activity, and the decrease in mucosal blood flow, and prevented mucosal injury induced by the electrical stimulation. These results support the concept that PAF is involved in granulocyte-mediated oxidative stress and gastric mucosal injury, and suggest that Rikkunshi-To prevents mucosal microvascular injury and formation of hemorrhagic lesion by modulating PAF production and oxidative granulocyte activation.
| Original language | English |
|---|---|
| Pages (from-to) | 153-159 |
| Number of pages | 7 |
| Journal | Pathophysiology |
| Volume | 2 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1995 |
Fingerprint
Keywords
- Luminol-dependent chemiluminescence
- Microcirculation
- Myeloperoxidase (MPO)
- Oxygen radicals
- Superoxide dismutase (SOD)
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Physiology (medical)
Cite this
Inhibitions of platelet-activating factor production and granulocyte oxidative activation by Rikkunshi-To in the process of gastric mucosal injury. / Kurose, Iwao; Miura, Soichiro; Fukumura, Dai; Suzuki, Hidekazu; Tsuchiya, Masaharu; Ishii, Hiromasa.
In: Pathophysiology, Vol. 2, No. 3, 1995, p. 153-159.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibitions of platelet-activating factor production and granulocyte oxidative activation by Rikkunshi-To in the process of gastric mucosal injury
AU - Kurose, Iwao
AU - Miura, Soichiro
AU - Fukumura, Dai
AU - Suzuki, Hidekazu
AU - Tsuchiya, Masaharu
AU - Ishii, Hiromasa
PY - 1995
Y1 - 1995
N2 - The effects of Rikkunshi-To on gastric mucosal injury were investigated with attention to the mechanisms of oxidative stress. Repeated electrical stimulation (RES) on the gastric artery of male Wistar rats decreased mucosal blood flow, which was measured by laser Doppler flowmetry, and subsequently produced hemorrhagic lesions in the gastric mucosa. At 30 min after the completion of RES, increased platelet-activating factor (PAF) content and myeloperoxidase (MPO) activity were observed in the gastric mucosa. Luminol-dependent chemiluminescence activity in blood samples taken from a gastric vein was also elevated after the stimulation. Oral administration of Rikkunshi-To (50 mg/kg) attenuated the increases in luminol-dependent chemiluminescence activity, mucosal PAF content and MPO activity, and the decrease in mucosal blood flow, and prevented mucosal injury induced by the electrical stimulation. These results support the concept that PAF is involved in granulocyte-mediated oxidative stress and gastric mucosal injury, and suggest that Rikkunshi-To prevents mucosal microvascular injury and formation of hemorrhagic lesion by modulating PAF production and oxidative granulocyte activation.
AB - The effects of Rikkunshi-To on gastric mucosal injury were investigated with attention to the mechanisms of oxidative stress. Repeated electrical stimulation (RES) on the gastric artery of male Wistar rats decreased mucosal blood flow, which was measured by laser Doppler flowmetry, and subsequently produced hemorrhagic lesions in the gastric mucosa. At 30 min after the completion of RES, increased platelet-activating factor (PAF) content and myeloperoxidase (MPO) activity were observed in the gastric mucosa. Luminol-dependent chemiluminescence activity in blood samples taken from a gastric vein was also elevated after the stimulation. Oral administration of Rikkunshi-To (50 mg/kg) attenuated the increases in luminol-dependent chemiluminescence activity, mucosal PAF content and MPO activity, and the decrease in mucosal blood flow, and prevented mucosal injury induced by the electrical stimulation. These results support the concept that PAF is involved in granulocyte-mediated oxidative stress and gastric mucosal injury, and suggest that Rikkunshi-To prevents mucosal microvascular injury and formation of hemorrhagic lesion by modulating PAF production and oxidative granulocyte activation.
KW - Luminol-dependent chemiluminescence
KW - Microcirculation
KW - Myeloperoxidase (MPO)
KW - Oxygen radicals
KW - Superoxide dismutase (SOD)
UR - http://www.scopus.com/inward/record.url?scp=0029145721&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029145721&partnerID=8YFLogxK
U2 - 10.1016/0928-4680(95)00030-5
DO - 10.1016/0928-4680(95)00030-5
M3 - Article
AN - SCOPUS:0029145721
VL - 2
SP - 153
EP - 159
JO - Pathophysiology
JF - Pathophysiology
SN - 0928-4680
IS - 3
ER -