Inhibitory effect of a fullerene derivative, monomalonic acid C60, on nitric oxide-dependent relaxation of aortic smooth muscle

Mitsutoshi Satoh, Kazuhiro Matsuo, Hitoshi Kiriya, Tadahiko Mashino, Masaaki Hirobe, Issei Takayanagi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

1. Effects of a fullerene C60, derivative, monomalonic acid C60 (MMA C60), on endothelium-containing or denuded aorta of rabbit, trachea and ileum of guinea pig, and stomach (fundus), vas deferens and uterus of rat were studied pharmacologically. 2. MMA C60 (10-5 M) significantly reduced the maximum response of the relaxation induced by acetylcholine in endothelium containing thoracic aorta of rabbit, and the acetylcholine induced relaxation was recovered in the presence of superoxide dismutase (SOD, 250 units/ml). 3. Nitric oxide-generating agent, S-nitroso-N-acetylpenicillamine, caused the relaxation of aorta without endothelium in a concentration dependent manner, and the concentration-response curve was shifted to the right in the presence of MMA C60. This inhibitory effect of the derivative was also masked in the presence of superoxide dismutase (SOD). 4. Sodium nitroprusside-induced relaxation was not affected by either MMA C60 Or SOD. In the other tissues, this C60 derivative had no effect on the responses induced by any agonist. 5. These observations indicate that MMA C60 inhibits the endothelium dependent relaxation induced by acetylcholine but does not affect the agonist-induced contractile response of smooth muscle.

Original languageEnglish
Pages (from-to)345-351
Number of pages7
JournalGeneral Pharmacology
Volume29
Issue number3
DOIs
Publication statusPublished - 1997 Sep
Externally publishedYes

Fingerprint

Fullerenes
Smooth Muscle
Nitric Oxide
Superoxide Dismutase
Endothelium
Acids
Acetylcholine
Aorta
S-Nitroso-N-Acetylpenicillamine
Rabbits
Vas Deferens
Nitroprusside
Trachea
Thoracic Aorta
Ileum
Uterus
Stomach
Guinea Pigs

Keywords

  • Endothelium
  • Monomalonic acid C (MMA C)
  • Nitric oxide (NO)
  • Smooth muscle
  • Superoxide dismutase (SOD)

ASJC Scopus subject areas

  • Pharmacology

Cite this

Inhibitory effect of a fullerene derivative, monomalonic acid C60, on nitric oxide-dependent relaxation of aortic smooth muscle. / Satoh, Mitsutoshi; Matsuo, Kazuhiro; Kiriya, Hitoshi; Mashino, Tadahiko; Hirobe, Masaaki; Takayanagi, Issei.

In: General Pharmacology, Vol. 29, No. 3, 09.1997, p. 345-351.

Research output: Contribution to journalArticle

Satoh, Mitsutoshi ; Matsuo, Kazuhiro ; Kiriya, Hitoshi ; Mashino, Tadahiko ; Hirobe, Masaaki ; Takayanagi, Issei. / Inhibitory effect of a fullerene derivative, monomalonic acid C60, on nitric oxide-dependent relaxation of aortic smooth muscle. In: General Pharmacology. 1997 ; Vol. 29, No. 3. pp. 345-351.
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