Inhibitory effect of hericenone B from Hericium erinaceus on collagen-induced platelet aggregation

Koichiro Mori, Haruhisa Kikuchi, Yutaro Obara, Masaya Iwashita, Yoshihito Azumi, Satomi Kinugasa, Satoshi Inatomi, Yoshiteru Oshima, Norimichi Nakahata

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Platelet aggregation in the blood vessel causes thrombosis. Therefore, inhibitors of platelet aggregation promise to be preventive or therapeutic agents of various vascular diseases, including myocardial infarction and stroke. In the present study, we found that hericenone B had a strong anti-platelet activity and it might be a novel compound for antithrombotic therapy possessing a novel mechanism. Prior to this study, we examined anti-platelet aggregation activity of ethanol extracts of several species of mushrooms, and found that extract of Hericium erinaceus potently inhibited platelet aggregation induced by collagen. Therefore, we first fractionated the ethanol extract of H. erinaceus to identify the active substances. The anti-platelet activity of each fraction was determined using washed rabbit platelets. As a result, an active component was isolated and identified as hericenone B. Hericenone B selectively inhibited collagen-induced platelet aggregation, but it did not suppress the aggregation induced by U46619 (TXA2 analogue), ADP, thrombin, or adrenaline. Furthermore, hericenone B did not inhibit arachidonic acid- or convulxin (GPVI agonist)-induced platelet aggregation. Therefore, hericenone B was considered to block collagen signaling from integrin α2/β1 to arachidonic acid release. Moreover, we found that collagen-induced aggregation was inhibited by hericenone B in human platelets, similar to in rabbit platelets.

Original languageEnglish
Pages (from-to)1082-1085
Number of pages4
JournalPhytomedicine
Volume17
Issue number14
DOIs
Publication statusPublished - 2010 Dec 1
Externally publishedYes

Keywords

  • Collagen
  • Hericenone
  • Hericium erinaceus
  • Integrin α2/β1
  • Platelet aggregation

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

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