Inhibitory effect of N,N-didesmethylgrossularine-1 on inflammatory cytokine production in lipopolysaccharide-stimulated RAW 264.7 cells

Taiko Oda, Jong Soo Lee, Yuta Sato, Yasuaki Kabe, Satoshi Sakamoto, Hiroshi Handa, Remy E.P. Mangindaan, Michio Namikoshi

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

N,N-Didesmethylgrossularine-1 (DDMG-1), a compound with a rare α-carboline structure, was isolated from an Indonesian ascidian Polycarpa aurata as responsible for the observed inhibitory activity against TNF-α production in lipopolysaccharide-stimulated murine macrophage-like RAW264.7 cells. DDMG-1 inhibited the mRNA level of mTNF-α, IκB-α degradation, and binding of NF-κB to the target DNA site in LPS-stimulated RAW 264.7 cells. Moreover, DDMG-1 had an inhibitory effect on the production of IL-8, which is produced in CD14 +-THP-1 cells stimulated by LPS. DDMG-1 is thus a promising drug candidate lead compound for the treatment of chronic inflammatory diseases, such as rheumatoid arthritis.

Original languageEnglish
Pages (from-to)589-599
Number of pages11
JournalMarine Drugs
Volume7
Issue number4
DOIs
Publication statusPublished - 2009 Dec

Keywords

  • LPS
  • N,N-didesmethylgrossularine-1
  • NF-κB
  • Natural product
  • Raw 264.7 cells
  • TNF-α

ASJC Scopus subject areas

  • Drug Discovery

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