Inhibitory effect of N,N-didesmethylgrossularine-1 on inflammatory cytokine production in lipopolysaccharide-stimulated RAW 264.7 cells

Taiko Oda; Jong Soo Lee; Yuta Sato; Yasuaki Kabe; Satoshi Sakamoto; Hiroshi Handa; Remy E.P. Mangindaan; Michio Namikoshi

doi:10.3390/md7040589

Inhibitory effect of N,N-didesmethylgrossularine-1 on inflammatory cytokine production in lipopolysaccharide-stimulated RAW 264.7 cells

Taiko Oda, Jong Soo Lee, Yuta Sato, Yasuaki Kabe, Satoshi Sakamoto, Hiroshi Handa, Remy E.P. Mangindaan, Michio Namikoshi

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

N,N-Didesmethylgrossularine-1 (DDMG-1), a compound with a rare α-carboline structure, was isolated from an Indonesian ascidian Polycarpa aurata as responsible for the observed inhibitory activity against TNF-α production in lipopolysaccharide-stimulated murine macrophage-like RAW264.7 cells. DDMG-1 inhibited the mRNA level of mTNF-α, IκB-α degradation, and binding of NF-κB to the target DNA site in LPS-stimulated RAW 264.7 cells. Moreover, DDMG-1 had an inhibitory effect on the production of IL-8, which is produced in CD14⁺-THP-1 cells stimulated by LPS. DDMG-1 is thus a promising drug candidate lead compound for the treatment of chronic inflammatory diseases, such as rheumatoid arthritis.

Original language	English
Pages (from-to)	589-599
Number of pages	11
Journal	Marine Drugs
Volume	7
Issue number	4
DOIs	https://doi.org/10.3390/md7040589
Publication status	Published - 2009 Dec
Externally published	Yes

Keywords

LPS
N,N-didesmethylgrossularine-1
NF-κB
Natural product
Raw 264.7 cells
TNF-α

ASJC Scopus subject areas

Drug Discovery

Access to Document

10.3390/md7040589

Cite this

@article{06969f622fed459f9e43d9842c235a39,

title = "Inhibitory effect of N,N-didesmethylgrossularine-1 on inflammatory cytokine production in lipopolysaccharide-stimulated RAW 264.7 cells",

abstract = "N,N-Didesmethylgrossularine-1 (DDMG-1), a compound with a rare α-carboline structure, was isolated from an Indonesian ascidian Polycarpa aurata as responsible for the observed inhibitory activity against TNF-α production in lipopolysaccharide-stimulated murine macrophage-like RAW264.7 cells. DDMG-1 inhibited the mRNA level of mTNF-α, IκB-α degradation, and binding of NF-κB to the target DNA site in LPS-stimulated RAW 264.7 cells. Moreover, DDMG-1 had an inhibitory effect on the production of IL-8, which is produced in CD14+-THP-1 cells stimulated by LPS. DDMG-1 is thus a promising drug candidate lead compound for the treatment of chronic inflammatory diseases, such as rheumatoid arthritis.",

keywords = "LPS, N,N-didesmethylgrossularine-1, NF-κB, Natural product, Raw 264.7 cells, TNF-α",

author = "Taiko Oda and Lee, {Jong Soo} and Yuta Sato and Yasuaki Kabe and Satoshi Sakamoto and Hiroshi Handa and Mangindaan, {Remy E.P.} and Michio Namikoshi",

year = "2009",

month = dec,

doi = "10.3390/md7040589",

language = "English",

volume = "7",

pages = "589--599",

journal = "Marine Drugs",

issn = "1660-3397",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "4",

}

TY - JOUR

T1 - Inhibitory effect of N,N-didesmethylgrossularine-1 on inflammatory cytokine production in lipopolysaccharide-stimulated RAW 264.7 cells

AU - Oda, Taiko

AU - Lee, Jong Soo

AU - Sato, Yuta

AU - Kabe, Yasuaki

AU - Sakamoto, Satoshi

AU - Handa, Hiroshi

AU - Mangindaan, Remy E.P.

AU - Namikoshi, Michio

PY - 2009/12

Y1 - 2009/12

N2 - N,N-Didesmethylgrossularine-1 (DDMG-1), a compound with a rare α-carboline structure, was isolated from an Indonesian ascidian Polycarpa aurata as responsible for the observed inhibitory activity against TNF-α production in lipopolysaccharide-stimulated murine macrophage-like RAW264.7 cells. DDMG-1 inhibited the mRNA level of mTNF-α, IκB-α degradation, and binding of NF-κB to the target DNA site in LPS-stimulated RAW 264.7 cells. Moreover, DDMG-1 had an inhibitory effect on the production of IL-8, which is produced in CD14+-THP-1 cells stimulated by LPS. DDMG-1 is thus a promising drug candidate lead compound for the treatment of chronic inflammatory diseases, such as rheumatoid arthritis.

AB - N,N-Didesmethylgrossularine-1 (DDMG-1), a compound with a rare α-carboline structure, was isolated from an Indonesian ascidian Polycarpa aurata as responsible for the observed inhibitory activity against TNF-α production in lipopolysaccharide-stimulated murine macrophage-like RAW264.7 cells. DDMG-1 inhibited the mRNA level of mTNF-α, IκB-α degradation, and binding of NF-κB to the target DNA site in LPS-stimulated RAW 264.7 cells. Moreover, DDMG-1 had an inhibitory effect on the production of IL-8, which is produced in CD14+-THP-1 cells stimulated by LPS. DDMG-1 is thus a promising drug candidate lead compound for the treatment of chronic inflammatory diseases, such as rheumatoid arthritis.

KW - LPS

KW - N,N-didesmethylgrossularine-1

KW - NF-κB

KW - Natural product

KW - Raw 264.7 cells

KW - TNF-α

UR - http://www.scopus.com/inward/record.url?scp=75149118321&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=75149118321&partnerID=8YFLogxK

U2 - 10.3390/md7040589

DO - 10.3390/md7040589

M3 - Article

C2 - 20098600

AN - SCOPUS:75149118321

SN - 1660-3397

VL - 7

SP - 589

EP - 599

JO - Marine Drugs

JF - Marine Drugs

IS - 4

ER -

Inhibitory effect of N,N-didesmethylgrossularine-1 on inflammatory cytokine production in lipopolysaccharide-stimulated RAW 264.7 cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this