Inhibitory effect of oxycoumarins isolated from the Thai medicinal plant Clausena guillauminii on the inflammation mediators, iNOS, TNF-α, and COX-2 expression in mouse macrophage RAW 264.7

Tomonori Nakamura, Naoko Kodama, Yu Arai, Takuya Kumamoto, Yoshihiro Higuchi, Chaiyo Chaichantipyuth, Tsutomu Ishikawa, Koichi Ueno, Shingo Yano

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

In the present study, we investigated the inhibitory effect of the known oxycoumarins poncitrin (3), osthol (4), and xanthoxyletin (5), newly isolated from Clausena guillauminii (Rutaceae), together with the known carbazoles heptaphylline (1) and 7-methoxyheptaphylline (2) on inducible-nitric oxide synthase (iNOS) expression induced by lipopolysaccharide (LPS) and the NO generation in RAW 264.7 mouse macrophages. Isolation of active oxycoumarins was guided by Western blot analysis of iNOS protein expression. These oxycoumarins showed an inhibitory effect on iNOS protein expression at 10 μM. Further examination of the inhibitory effects of these compounds on inflammation mediators revealed that the synthesis of nitric oxide (NO) and the protein expression of tumor necrosis factor-α (TNF-α) and cyclooxygenase-2 (COX-2) were inhibited by 5. It was expected that these compounds show anti-inflammatory activities.

Original languageEnglish
Pages (from-to)21-27
Number of pages7
JournalJournal of Natural Medicines
Volume63
Issue number1
DOIs
Publication statusPublished - 2009 Jan 1
Externally publishedYes

Keywords

  • Clausena guillauminii
  • Oxycoumarin
  • RAW 264.7
  • Rutaceae
  • Xanthoxyletin
  • iNOS

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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