Abstract
In the present study, we investigated the inhibitory effect of the known oxycoumarins poncitrin (3), osthol (4), and xanthoxyletin (5), newly isolated from Clausena guillauminii (Rutaceae), together with the known carbazoles heptaphylline (1) and 7-methoxyheptaphylline (2) on inducible-nitric oxide synthase (iNOS) expression induced by lipopolysaccharide (LPS) and the NO generation in RAW 264.7 mouse macrophages. Isolation of active oxycoumarins was guided by Western blot analysis of iNOS protein expression. These oxycoumarins showed an inhibitory effect on iNOS protein expression at 10 μM. Further examination of the inhibitory effects of these compounds on inflammation mediators revealed that the synthesis of nitric oxide (NO) and the protein expression of tumor necrosis factor-α (TNF-α) and cyclooxygenase-2 (COX-2) were inhibited by 5. It was expected that these compounds show anti-inflammatory activities.
| Original language | English |
|---|---|
| Pages (from-to) | 21-27 |
| Number of pages | 7 |
| Journal | Journal of Natural Medicines |
| Volume | 63 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2009 Jan |
| Externally published | Yes |
Fingerprint
Keywords
- Clausena guillauminii
- iNOS
- Oxycoumarin
- RAW 264.7
- Rutaceae
- Xanthoxyletin
ASJC Scopus subject areas
- Organic Chemistry
- Drug Discovery
- Pharmaceutical Science
- Complementary and alternative medicine
Cite this
Inhibitory effect of oxycoumarins isolated from the Thai medicinal plant Clausena guillauminii on the inflammation mediators, iNOS, TNF-α, and COX-2 expression in mouse macrophage RAW 264.7. / Nakamura, Tomonori; Kodama, Naoko; Arai, Yu; Kumamoto, Takuya; Higuchi, Yoshihiro; Chaichantipyuth, Chaiyo; Ishikawa, Tsutomu; Ueno, Koichi; Yano, Shingo.
In: Journal of Natural Medicines, Vol. 63, No. 1, 01.2009, p. 21-27.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibitory effect of oxycoumarins isolated from the Thai medicinal plant Clausena guillauminii on the inflammation mediators, iNOS, TNF-α, and COX-2 expression in mouse macrophage RAW 264.7
AU - Nakamura, Tomonori
AU - Kodama, Naoko
AU - Arai, Yu
AU - Kumamoto, Takuya
AU - Higuchi, Yoshihiro
AU - Chaichantipyuth, Chaiyo
AU - Ishikawa, Tsutomu
AU - Ueno, Koichi
AU - Yano, Shingo
PY - 2009/1
Y1 - 2009/1
N2 - In the present study, we investigated the inhibitory effect of the known oxycoumarins poncitrin (3), osthol (4), and xanthoxyletin (5), newly isolated from Clausena guillauminii (Rutaceae), together with the known carbazoles heptaphylline (1) and 7-methoxyheptaphylline (2) on inducible-nitric oxide synthase (iNOS) expression induced by lipopolysaccharide (LPS) and the NO generation in RAW 264.7 mouse macrophages. Isolation of active oxycoumarins was guided by Western blot analysis of iNOS protein expression. These oxycoumarins showed an inhibitory effect on iNOS protein expression at 10 μM. Further examination of the inhibitory effects of these compounds on inflammation mediators revealed that the synthesis of nitric oxide (NO) and the protein expression of tumor necrosis factor-α (TNF-α) and cyclooxygenase-2 (COX-2) were inhibited by 5. It was expected that these compounds show anti-inflammatory activities.
AB - In the present study, we investigated the inhibitory effect of the known oxycoumarins poncitrin (3), osthol (4), and xanthoxyletin (5), newly isolated from Clausena guillauminii (Rutaceae), together with the known carbazoles heptaphylline (1) and 7-methoxyheptaphylline (2) on inducible-nitric oxide synthase (iNOS) expression induced by lipopolysaccharide (LPS) and the NO generation in RAW 264.7 mouse macrophages. Isolation of active oxycoumarins was guided by Western blot analysis of iNOS protein expression. These oxycoumarins showed an inhibitory effect on iNOS protein expression at 10 μM. Further examination of the inhibitory effects of these compounds on inflammation mediators revealed that the synthesis of nitric oxide (NO) and the protein expression of tumor necrosis factor-α (TNF-α) and cyclooxygenase-2 (COX-2) were inhibited by 5. It was expected that these compounds show anti-inflammatory activities.
KW - Clausena guillauminii
KW - iNOS
KW - Oxycoumarin
KW - RAW 264.7
KW - Rutaceae
KW - Xanthoxyletin
UR - http://www.scopus.com/inward/record.url?scp=57049104672&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=57049104672&partnerID=8YFLogxK
U2 - 10.1007/s11418-008-0277-5
DO - 10.1007/s11418-008-0277-5
M3 - Article
VL - 63
SP - 21
EP - 27
JO - Journal of Natural Medicines
JF - Journal of Natural Medicines
SN - 1861-0293
IS - 1
ER -