Inhibitory effects of a fullerene derivative, dimalonic acid C60, on nitric oxide-induced relaxed of rabbit aorta

Mitsutoshi Satoh, Kazuhiro Matsuo, Hitoshi Kiriya, Tadahiko Mashino, Tetsuo Nagano, Masaaki Hirobe, Issei Takayanagi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Dimalonic acid C60 (10-5 M), a new fullerene derivative, produced an augmentation of phenylephrine-induced tone and reduced both the acetylcholine-induced maximum relaxation and the amplitude of substance P(10-8 M)-induced relaxation inendothelium-containing thoracic aorta of rabbit; the acetylcholine- and substance P-induced relaxation was restored in the presence of superoxide dismutase (250 U/ml). Dimalonic acid C60 (10-5 M) did not influence the phenylephrine-induced contractile response in the absence of endothelium, but the acetylcholine-induced relaxation was eliminated by removal of the endothelium. Superoxide anion generation, using hypoxantine (1mM)/xanthine oxidase (16mU/ml), reduced the acetylcholine-induced relaxation and produced an augmentation of phenylephrine-induced tone in endothelium-containing strips; these effects were negated by the addition of superoxide dismutase (250 U/ml). A nitric oxide-generation agent, S-nitroso-N-acetylpenicillamine, caused relaxation of aorta without endothelium in a concentration-dependent manner, and the concentration-response curve was shifted to the right in the presence of dimalonic acid C60. This inhibitory effect of dimalonic acid C60 was also masked in the presence of superoxide dismutase. Sodium nitroprusside-induced relaxation was not affected by either dimalonic acid C60 or superoxide dismutase. These observations suggests that dimalonic acid C60 or superoxide dismutase. These observations suggests that dimalonic acid C60 inhibits endothelium (nitric oxide)-dependent agonist-induced relaxation through the production of superoxide.

Original languageEnglish
Pages (from-to)176-181
Number of pages6
JournalEuropean Journal of Pharmacology
Volume327
Issue number2-3
Publication statusPublished - 1997 May 30

Fingerprint

Fullerenes
Aorta
Nitric Oxide
Rabbits
Superoxide Dismutase
Endothelium
Acetylcholine
Phenylephrine
Substance P
Superoxides
S-Nitroso-N-Acetylpenicillamine
Xanthine Oxidase
Nitroprusside
dimalonic acid C(60)
Thoracic Aorta

Keywords

  • Dimalonic acid C
  • Endothelium
  • Nitric oxide (NO)
  • Smooth muscle
  • Superoxide dismutase

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Satoh, M., Matsuo, K., Kiriya, H., Mashino, T., Nagano, T., Hirobe, M., & Takayanagi, I. (1997). Inhibitory effects of a fullerene derivative, dimalonic acid C60, on nitric oxide-induced relaxed of rabbit aorta. European Journal of Pharmacology, 327(2-3), 176-181.

Inhibitory effects of a fullerene derivative, dimalonic acid C60, on nitric oxide-induced relaxed of rabbit aorta. / Satoh, Mitsutoshi; Matsuo, Kazuhiro; Kiriya, Hitoshi; Mashino, Tadahiko; Nagano, Tetsuo; Hirobe, Masaaki; Takayanagi, Issei.

In: European Journal of Pharmacology, Vol. 327, No. 2-3, 30.05.1997, p. 176-181.

Research output: Contribution to journalArticle

Satoh, M, Matsuo, K, Kiriya, H, Mashino, T, Nagano, T, Hirobe, M & Takayanagi, I 1997, 'Inhibitory effects of a fullerene derivative, dimalonic acid C60, on nitric oxide-induced relaxed of rabbit aorta', European Journal of Pharmacology, vol. 327, no. 2-3, pp. 176-181.
Satoh, Mitsutoshi ; Matsuo, Kazuhiro ; Kiriya, Hitoshi ; Mashino, Tadahiko ; Nagano, Tetsuo ; Hirobe, Masaaki ; Takayanagi, Issei. / Inhibitory effects of a fullerene derivative, dimalonic acid C60, on nitric oxide-induced relaxed of rabbit aorta. In: European Journal of Pharmacology. 1997 ; Vol. 327, No. 2-3. pp. 176-181.
@article{8afbc908dcdc4a95a8709bc9bb9949ca,
title = "Inhibitory effects of a fullerene derivative, dimalonic acid C60, on nitric oxide-induced relaxed of rabbit aorta",
abstract = "Dimalonic acid C60 (10-5 M), a new fullerene derivative, produced an augmentation of phenylephrine-induced tone and reduced both the acetylcholine-induced maximum relaxation and the amplitude of substance P(10-8 M)-induced relaxation inendothelium-containing thoracic aorta of rabbit; the acetylcholine- and substance P-induced relaxation was restored in the presence of superoxide dismutase (250 U/ml). Dimalonic acid C60 (10-5 M) did not influence the phenylephrine-induced contractile response in the absence of endothelium, but the acetylcholine-induced relaxation was eliminated by removal of the endothelium. Superoxide anion generation, using hypoxantine (1mM)/xanthine oxidase (16mU/ml), reduced the acetylcholine-induced relaxation and produced an augmentation of phenylephrine-induced tone in endothelium-containing strips; these effects were negated by the addition of superoxide dismutase (250 U/ml). A nitric oxide-generation agent, S-nitroso-N-acetylpenicillamine, caused relaxation of aorta without endothelium in a concentration-dependent manner, and the concentration-response curve was shifted to the right in the presence of dimalonic acid C60. This inhibitory effect of dimalonic acid C60 was also masked in the presence of superoxide dismutase. Sodium nitroprusside-induced relaxation was not affected by either dimalonic acid C60 or superoxide dismutase. These observations suggests that dimalonic acid C60 or superoxide dismutase. These observations suggests that dimalonic acid C60 inhibits endothelium (nitric oxide)-dependent agonist-induced relaxation through the production of superoxide.",
keywords = "Dimalonic acid C, Endothelium, Nitric oxide (NO), Smooth muscle, Superoxide dismutase",
author = "Mitsutoshi Satoh and Kazuhiro Matsuo and Hitoshi Kiriya and Tadahiko Mashino and Tetsuo Nagano and Masaaki Hirobe and Issei Takayanagi",
year = "1997",
month = "5",
day = "30",
language = "English",
volume = "327",
pages = "176--181",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Inhibitory effects of a fullerene derivative, dimalonic acid C60, on nitric oxide-induced relaxed of rabbit aorta

AU - Satoh, Mitsutoshi

AU - Matsuo, Kazuhiro

AU - Kiriya, Hitoshi

AU - Mashino, Tadahiko

AU - Nagano, Tetsuo

AU - Hirobe, Masaaki

AU - Takayanagi, Issei

PY - 1997/5/30

Y1 - 1997/5/30

N2 - Dimalonic acid C60 (10-5 M), a new fullerene derivative, produced an augmentation of phenylephrine-induced tone and reduced both the acetylcholine-induced maximum relaxation and the amplitude of substance P(10-8 M)-induced relaxation inendothelium-containing thoracic aorta of rabbit; the acetylcholine- and substance P-induced relaxation was restored in the presence of superoxide dismutase (250 U/ml). Dimalonic acid C60 (10-5 M) did not influence the phenylephrine-induced contractile response in the absence of endothelium, but the acetylcholine-induced relaxation was eliminated by removal of the endothelium. Superoxide anion generation, using hypoxantine (1mM)/xanthine oxidase (16mU/ml), reduced the acetylcholine-induced relaxation and produced an augmentation of phenylephrine-induced tone in endothelium-containing strips; these effects were negated by the addition of superoxide dismutase (250 U/ml). A nitric oxide-generation agent, S-nitroso-N-acetylpenicillamine, caused relaxation of aorta without endothelium in a concentration-dependent manner, and the concentration-response curve was shifted to the right in the presence of dimalonic acid C60. This inhibitory effect of dimalonic acid C60 was also masked in the presence of superoxide dismutase. Sodium nitroprusside-induced relaxation was not affected by either dimalonic acid C60 or superoxide dismutase. These observations suggests that dimalonic acid C60 or superoxide dismutase. These observations suggests that dimalonic acid C60 inhibits endothelium (nitric oxide)-dependent agonist-induced relaxation through the production of superoxide.

AB - Dimalonic acid C60 (10-5 M), a new fullerene derivative, produced an augmentation of phenylephrine-induced tone and reduced both the acetylcholine-induced maximum relaxation and the amplitude of substance P(10-8 M)-induced relaxation inendothelium-containing thoracic aorta of rabbit; the acetylcholine- and substance P-induced relaxation was restored in the presence of superoxide dismutase (250 U/ml). Dimalonic acid C60 (10-5 M) did not influence the phenylephrine-induced contractile response in the absence of endothelium, but the acetylcholine-induced relaxation was eliminated by removal of the endothelium. Superoxide anion generation, using hypoxantine (1mM)/xanthine oxidase (16mU/ml), reduced the acetylcholine-induced relaxation and produced an augmentation of phenylephrine-induced tone in endothelium-containing strips; these effects were negated by the addition of superoxide dismutase (250 U/ml). A nitric oxide-generation agent, S-nitroso-N-acetylpenicillamine, caused relaxation of aorta without endothelium in a concentration-dependent manner, and the concentration-response curve was shifted to the right in the presence of dimalonic acid C60. This inhibitory effect of dimalonic acid C60 was also masked in the presence of superoxide dismutase. Sodium nitroprusside-induced relaxation was not affected by either dimalonic acid C60 or superoxide dismutase. These observations suggests that dimalonic acid C60 or superoxide dismutase. These observations suggests that dimalonic acid C60 inhibits endothelium (nitric oxide)-dependent agonist-induced relaxation through the production of superoxide.

KW - Dimalonic acid C

KW - Endothelium

KW - Nitric oxide (NO)

KW - Smooth muscle

KW - Superoxide dismutase

UR - http://www.scopus.com/inward/record.url?scp=0030913773&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030913773&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0030913773

VL - 327

SP - 176

EP - 181

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 2-3

ER -