Inhibitory effects of herbal extracts on the activity of human sulfotransferase isoform sulfotransferase 1A3 (SULT1A3)

Marie Nagai, Takahiro Fukamachi, Masayuki Tsujimoto, Kenichiro Ogura, Akira Hiratsuka, Hisakazu Ohtani, Satoko Hori, Yasufumi Sawada

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Sulfotransferase 1A3 (SULT1A3) is a phase II detoxifying enzyme of xenobiotics predominantly expressed in the intestinal epithelium. Recent increase in the use of herbal extracts as dietary supplements may lead to an increase in the possibility of dietary supplement-drug interactions. The purpose of the present study was to investigate the effects of 18 herbal extracts on SULT1A3 activity and the possibility of interaction between medicinal drugs and herbal extracts. We examined the inhibitory potencies of 18 herbal extracts on the sulfation of dopamine, a typical substrate of SULT1A3, and ritodrine, a β 2 stimulant, by human recombinant SULT1A3. The sulfation of dopamine was inhibited by extracts of banaba, green tea, Rafuma, grape seed, peanut seed coat, gingko biloba leaf, St. John's wort, gymnema and milkthistle. The IC 50 values of these herbal extracts were lower than the putative gastrointestinal concentration when the recommended dose was ingested. On the other hand, chlorella extract and rutin showed no inhibitory effects and wheat, mulberry and siberian ginseng had IC 50 values exceedingly higher than the putative gastrointestinal concentration. The inhibitory profiles of herbal extracts for the sulfation of ritodrine were comparable to those for the sulfation of dopamine. In conclusion, the extracts of herbs such as banaba and green tea potently inhibited SULT1A3 activity. These extracts may increase the bioavailability of drugs whose bioavailabilities were limited by the function of SULT1A3 on the intestinal epithelium.

Original languageEnglish
Pages (from-to)105-109
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume32
Issue number1
DOIs
Publication statusPublished - 2009 Jan
Externally publishedYes

Fingerprint

Sulfotransferases
Human Activities
Protein Isoforms
Ritodrine
Dopamine
Tea
Intestinal Mucosa
Dietary Supplements
Gymnema
Biological Availability
Seeds
Eleutherococcus
Hypericum
Chlorella
Morus
Ginkgo biloba
Rutin
Vitis
Xenobiotics
Drug Interactions

Keywords

  • Food-drug interaction
  • Herbal extract
  • Metabolic inhibition
  • Sulfation

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

Inhibitory effects of herbal extracts on the activity of human sulfotransferase isoform sulfotransferase 1A3 (SULT1A3). / Nagai, Marie; Fukamachi, Takahiro; Tsujimoto, Masayuki; Ogura, Kenichiro; Hiratsuka, Akira; Ohtani, Hisakazu; Hori, Satoko; Sawada, Yasufumi.

In: Biological and Pharmaceutical Bulletin, Vol. 32, No. 1, 01.2009, p. 105-109.

Research output: Contribution to journalArticle

Nagai, Marie ; Fukamachi, Takahiro ; Tsujimoto, Masayuki ; Ogura, Kenichiro ; Hiratsuka, Akira ; Ohtani, Hisakazu ; Hori, Satoko ; Sawada, Yasufumi. / Inhibitory effects of herbal extracts on the activity of human sulfotransferase isoform sulfotransferase 1A3 (SULT1A3). In: Biological and Pharmaceutical Bulletin. 2009 ; Vol. 32, No. 1. pp. 105-109.
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